Expression of different classes of immunoglobulin in intraepithelial plasma cells of the Harderian gland of domestic ducks Anas platyrhynchos

Oliveira, Cleida A.; Telles, Luiz Flávio; Oliveira, André G.; Kalapothakis, Evanguedes; Gonçalves-Dornelas, Higgor; Mahecha, Germán Arturo Bohórquez

doi:10.1016/j.vetimm.2006.05.008

Cited by 13 publications

(13 citation statements)

References 46 publications

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“…The studies of HG include different bird species, including of birds also primitive avian species (Oliveira et al, 2006). In the present study, the HG of two popular birds from Phasianidae family of the Galli order were examined.…”

Section: Discussionmentioning

confidence: 99%

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Kowalczyk

et al. 2016

Rev. Bras. Cienc. Avic.

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Harderian gland, histology, histochemistry, common pheasant, hybrid of Italian amber and common pheasant. ABStRACtThe aim of the present study was to demonstrate the histological and histochemical structure of the Harderian gland in wild and hybrid of wild and domestic birds. The samples were stained with haematoxylin-eosin, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialyzed iron staining's. In both species, the glands had multilobar tubuloacinar structure type I. The Harderian gland was located in the orbit near the inter-orbital septum, between the medial rectus muscle, the pyramidal muscle of the third eyelid, and the ventral oblique muscle. In the common pheasant, the gland was wider in the proximal and distal part. The common pheasant had more elongated lobes of the Harderian gland than in the hybrid. In the common pheasant, the glandular cells presented darkly-stained serous secretion and lightly-stained mucous secretion. In the hybrid, the glandular cells presented seromucous secretion. The central lobar space, interacinar space, and apical parts of the acini of the Harderian glands were filled with many lymphocytes and plasma cells, particularly in the common pheasant, where centers of all large lobes were abundantly filled with plasma cells. The plasma cells dominated in common pheasant's Harderian gland, while in the hybrid, lymphocytes and plasma cells were present at similar quantities. The cells positive for periodic acid of Schiff staining were dominant in hybrid. Periodic acid-Schiff, Hale's dialyzed iron and alcian blue pH 2.5 stainings demonstrated acid-carboxylated mucopolysaccharides in the glandular cells cytoplasm of the examined birds.

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Section: Discussionmentioning

confidence: 99%

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Kowalczyk

et al. 2016

Rev. Bras. Cienc. Avic.

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“…The frequency of TRPV6 positive cells within the epididymal region was determined in the immunohistochemical slides, according to the literature (Oliveira et al 2006). For this purpose, it was counted the immunoreactive cells per 100 epithelial nonciliated cells in ten randomly selected longitudinal sections of the proximal efferent ductules.…”

Section: Quantitative Studiesmentioning

confidence: 99%

Involvement of the transepithelial calcium transport disruption and the formation of epididymal stones in roosters

Oliveira¹,

Aquino²,

Mahecha³

et al. 2012

Reproduction

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Epididymal lithiasis is a dysfunction of unknown origin characterized by the formation of calcium stones into the lumen of efferent ductules of roosters. Affected animals present an imbalance in the hormonal responsive systems that regulate the expression of proteins involved in the transepithelial calcium transport, as TRPV6, CaBP-D28K, NCX1, and PMCA. Because the efferent ductules are the major site of fluid and calcium reabsorption in excurrent ducts, it was hypothesized that impairment in local calcium homeostasis would lead to lithiasis. To test this hypothesis, we addressed the expression of these proteins in the epididymal region of affected animals. The present study focused on the investigation of the occurrence, tissue distribution, and physiological impact of the transepithelial calcium transport in roosters under normal and pathological conditions. The results showed that affected roosters presented a significant increase in TRPV6 and CaBP-D28k levels, whereas NCX1 and PMCA were not changed. Such alterations were more conspicuous in the proximal efferent ductules, in which was also observed accumulation of calcium within the epithelial cells. These findings provided the first evidences for the involvement of alteration in the expression of proteins essential for calcium reabsorption as a plausible mechanism for the formation of calcium stones within efferent ductules.

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“…The profile of bands with 2 major fragments was similar to that in reports of other bird species where the heavy chains and light chains were identified at 65-70 and 22-30 kDa, respectively. 4,5,14 Although the heavy and light chains were easily identified in all species tested, samples from most birds had greater reactivity with the light chains ( Figures 1B and 2A).…”

Section: Introductionmentioning

confidence: 99%

“…By use of Western blots, others have confirmed that anti‐chicken IgY antisera reacts with the light and heavy chains of immunoglobulins of other bird species such as raptors, ducks, and budgerigars (Table 1). 4–6 Others have successfully used anti‐chicken antibody in ELISA and immunohistochemistry systems for the detection of antibodies from raptors, penguins, herons, turkeys, ducks, quail, and psittacines 5,7–11 . A polyclonal antisera developed from immunization of goats with purified IgY from doves, ducks, sparrows, and chickens had excellent reactivity with 12 avian orders in ELISA systems 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Cross‐reactivity of anti‐chicken IgY antibody with immunoglobulins of exotic avian species

Cray

Villar

2008

Veterinary Clinical Pathol

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Expression of different classes of immunoglobulin in intraepithelial plasma cells of the Harderian gland of domestic ducks Anas platyrhynchos

Cited by 13 publications

References 46 publications

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Involvement of the transepithelial calcium transport disruption and the formation of epididymal stones in roosters

Cross‐reactivity of anti‐chicken IgY antibody with immunoglobulins of exotic avian species

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