Expression of E-cadherin in pig kidney

Lee, Su Youn; Han, Sun Young; Kim, Ji Eun; Chung, Ku Yong; Han, Ki Hwan

doi:10.4142/jvs.2013.14.4.381

Cited by 14 publications

(16 citation statements)

References 27 publications

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“…AQP1 is expressed in the S2 and S3 segments of the proximal tubule and the DTL (39,55). E-CAD is expressed in Bowman's capsule of the glomerulus, is not expressed in the AQP-1 proximal tubule, but is also expressed in the TAL, DCT, and CCD of the distal nephron (33,40,47). All three proximal tubule markers are detected in the HAK-APN cells, but the distal nephron marker E-CAD was not detected (Fig.…”

Section: Characterization Of Immunoaffinity-isolated Primary Human Kimentioning

confidence: 99%

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells

Emlet

Pastor-Soler

Marciszyn

et al. 2017

American Journal of Physiology-Renal Physiology

111

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We have characterized the expression and secretion of the acute kidney injury (AKI) biomarkers insulin-like growth factor binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human kidney epithelial cells in primary cell culture and tissue. We established cell culture model systems of primary kidney cells of proximal and distal tubule origin and observed that both proteins are indeed expressed and secreted in both tubule cell types in vitro. However, TIMP-2 is both expressed and secreted preferentially by cells of distal tubule origin, while IGFBP7 is equally expressed across tubule cell types yet preferentially secreted by cells of proximal tubule origin. In human kidney tissue, strong staining of IGFBP7 was seen in the luminal brush-border region of a subset of proximal tubule cells, and TIMP-2 stained intracellularly in distal tubules. Additionally, while some tubular colocalization of both biomarkers was identified with the injury markers kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin, both biomarkers could also be seen alone, suggesting the possibility for differential mechanistic and/or temporal profiles of regulation of these early AKI biomarkers from known markers of injury. Last, an in vitro model of ischemia-reperfusion demonstrated enhancement of secretion of both markers early after reperfusion. This work provides a rationale for further investigation of these markers for their potential role in the pathogenesis of acute kidney injury.

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Section: Characterization Of Immunoaffinity-isolated Primary Human Kimentioning

confidence: 99%

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells

Emlet

Pastor-Soler

Marciszyn

et al. 2017

American Journal of Physiology-Renal Physiology

111

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“…Claudin-1 and E-cadherin are important component proteins of tight junctions and adhesion junctions, respectively, which control the intestinal epithelium paracellular pathway (Tang & Goodenough, 2003;Lee et al, 2013). The tight junction is important in regulating intestinal inflammation.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Low Protein on Colonic Mucosal Barrier in Pigs

Hou

Mao

et al. 2017

Int. J. Morphol.

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SUMMARY:Reducing nitrogen nutrients concentration in dairy food is economic for pig industry. Here, we used finishing pig as model to investigate the effect on colon mucosal barrier and nutrients absorption after reducing crude protein (CP) in dietary from 16 % to 13 %. The results showed that crypt depth, cells, claudin-1 and E-cadherin expression level will not be changed, which implied the integrity of colon mucosal structure. Furthermore, the expressions of ASCT1, EAAT3 and SGLT1 in colon were also maintained at normal levels in 13 % CP dietary. Interestingly, the CAT1 and GLUT2 expression were increased significantly after reducing CP level to 13 %, which might be attributed to the compensatory nutrients absorption. This study implied that 13 % CP was sufficient to maintain normal colon structure and will not change intestinal morphology, which provided a basis for an ideal economic protein feed formula.

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“…Kidney tissue was also studied by electron microscopy. Fixed tissues were cut on a vibratome section system (Intracel, UK) and processed for the pre-embedding horseradish peroxidase technique in accordance with our previous studies [ 10 11 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Kidney tissues were processed for immunoblot analysis as previously described [ 9 10 11 17 18 ]. Briefly, tissues from the renal cortex and medulla were homogenized in lysis buffer containing 20 mM Tris-HCl, 1% Triton X-100, 150 mM NaCl, 0.5% sodium deoxycholate, 10 mM leupeptin, 0.1% SDS, 1 mM EDTA, 0.02% NaN 3 , and 1 mM PMSF.…”

Section: Methodsmentioning

confidence: 99%

Hydration status affects osteopontin expression in the rat kidney

Lee

Piao

et al. 2016

J Vet Sci

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Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression. Under control conditions, OPN protein and mRNA expression were only detected in the descending thin limb (DTL). Dehydration induced increased expression in the DTL and the development of detectable expression in the thick ascending limb (TAL). In contrast, OPN expression levels declined to less than the controls in the DTL after hydration, while no expression of either protein or mRNA was detectable in the TAL. Immunoelectron microscopy demonstrated that hydration status altered tubular ultrastructure and intracellular OPN expression in the Golgi apparatus and secretory cytoplasmic vesicles. These data confirm that changes in oral fluid intake can regulate renal tubular epithelial cell OPN expression.

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Expression of E-cadherin in pig kidney

Cited by 14 publications

References 27 publications

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells

The Effect of Low Protein on Colonic Mucosal Barrier in Pigs

Hydration status affects osteopontin expression in the rat kidney

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