Expression of extracellular matrix molecules, MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments during rat tooth eruption

Abstract

Tooth eruption involves extensive degradation and reorganization of extracellular matrix (ECM) components. It is not known how ECM-degrading enzymes are coordinated with each other or how they are regulated in the event. The present study was designed to investigate mRNA expression of inhibitors of metalloproteinases (TIMPs) in comparison with matrix metalloproteinases (MMPs) as well as ECM molecules during rat first molar eruption using in situ hybridization. We also examined how TIMPs are involved in the process of tooth eruption, root formation, cementogenesis and alveolar bone remodelling. Expressions of type-I collagen, osteocalcin, MMPs 2 and 8, and TIMPs 1, 2 and 3 were shown in osteoblasts, osteocytes, cementoblasts, cementocytes and periodontal ligament fibroblasts, and the concomitant high expressions of the ECM molecules, MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments were identified in the middle of first molar eruption. The remodelling of ECM in these periodontal tissues might be regulated through balance among the production of ECM molecules, the degradation of ECM by MMPs and the inhibition of MMPs by TIMPs during tooth eruption.

Quantitative Analysis and Localization of mRNA Transcripts of Type I Collagen, Osteocalcin, MMP 2, MMP 8, and MMP 13 During Bone Healing in a Rat Calvarial Experimental Defect Model

“…Osteoblasts and osteocytes may play a role in remodeling of the ECM during bone healing by coordinating the production and degradation of ECM proteins. Similar observations have been reported during bone development Tsubota et al, 2002;Maruya et al, 2003;Nakamura et al, 2005;Sone et al, 2005) and during appositional bone formation (Hatori et al, 2004).…”

“…MMPs 2, 8, and 13 have been implicated in the degradation of ECM during bone development (Gack et al, 1995;Mattot et al, 1995;Chin and Werb, 1997;Johansson et al, 1997;Sasano et al, 2002;Maruya et al, 2003). MMPs 2, 8, 9, 13, and 14 have been shown to participate in the healing of bone fracture (Yamagiwa et al, 1999;Gerstenfeld et al, 2003;Henle et al, 2005;Wang et al, 2006).…”

Gene expression changes in bioceramic paste-treated human dental pulp cells

“…It has also been postulated that remodeling of proteoglycans may contribute to tooth eruption (12,13). The present study found that the expression of ADAMTS5 decreases after 24 h by 2.1 fold, suggesting that iRoot BP may have a negative effect on the remodeling of proteoglycans which constitute the extracellular environment of the teeth.…”

PrPc is temporospatially expressed in molar development of rats

“…PrP c recruits and couples to the CREB transcription factor and is involved in the transcriptional regulation of metalloproteinase MMP-9 and its inhibitor TIMP-1 in neuronal cells (Pradines et al, 2008). MMPs and TIMPs are also expressed in periodontal tissues including cementum and periodontal ligaments (Maruya et al, 2003) and involve in dentin matrix remodeling during tooth development (Goldberg et al, 2003;Sakuraba et al, 2006;Niu et al, 2011). A functional relationship of PrP c with these matrix enzymes in tooth development can be a challenging subject.…”