Expression of Fibronectin Isoforms Bearing the Alternatively Spliced EIIIA, EIIIB, and V Segments in Corneal Alkali Burn and Keratectomy Wound Models in the Rat

Havrlikova, Katerina; Mellott, Mei; Kaufman, Adam H.; Loredo, Grace A.; Peters, John H.; Colvin, Robert B.; Foster, C. Stephen

doi:10.1097/01.ico.0000133988.34274.24

Cited by 9 publications

(3 citation statements)

References 23 publications

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“…Muro et al found that mice without the EDA exon in the FN protein displayed abnormal skin wound healing, whereas mice having constitutive inclusion of the EDA exon showed a major decrease in the FN levels in all tissues [19]. In corneal alkali burn and keratectomy wound, it is suggest that Fn-EIIIA play a role in the high prevalence of recurrent epithelial erosions in alkali-wounded corneas [20]. In our study, the expressions of EIIIA in skin were gradually increased and displayed linear relationship with the injury time prolonging, as a hint for skin wound estimation.…”

Section: Discussionmentioning

confidence: 96%

Fibronectin EIIIA splicing variant: A useful contribution to forensic wounding interval estimation

Liu

Chen

Huang

2006

Forensic Science International

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Section: Discussionmentioning

confidence: 96%

Fibronectin EIIIA splicing variant: A useful contribution to forensic wounding interval estimation

Liu

Chen

Huang

2006

Forensic Science International

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“…Alkali burn was performed by touching a 4.0-mm-diameter applicator (a piece of sterile gauze) saturated with 1mol/l sodium hydroxide on the corneas of the left eyes of the two groups for 30 seconds. The ocular surface was then rinsed with 30ml sterile sodium chloride [2,[9][10][11].…”

Section: Methodsmentioning

confidence: 99%

Light and electron microscopic study of the corneal stroma during the healing process of alkali-induced ulcer

Mohamed¹

2012

The Egyptian Journal of Histology

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ResultsTwo weeks after alkali burn, corneal stromal neovascularization with the appearance of postcapillary venules was the most characteristic feature. This was associated with wide stromal spaces, inflammatory cellular infiltrate, activated fibroblasts, and irregularly deposited collagen. ConclusionDespite corneal reepithelialization, stromal changes might account for defective clarity following exposure to alkali.

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“…Enhanced alternative splicing of Fn1 variants were observed in cutaneous wounds, [24][25][26] corneal wounds, [27][28][29][30] gastric ulcer healing, 31 liver wound repair 32 and hepatic fibrogenesis, 33 myocardial infarction, 34 acute major trauma, 35 proliferative glomerulonephritis, 36,37 and peripheral nerve injury. 38 Assays of the plasma fibronectin and fetal fibronectin variants have been applied to the clinical diagnosis and therapeutic administration of fibronectin to human diseases.…”

Section: Commentmentioning

confidence: 99%

Age-Dependent Differential Expression of Fibronectin Variants in Skin and Airway Mucosal Wounds

Li-Korotky

Hebda²,

Lo³

et al. 2007

Arch Otolaryngol Head Neck Surg

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To delineate age-dependent and tissuespecific molecular activities of the variant-inclusion fibronectin transcripts in fetal and postnatal skin and airway mucosal wounds during early events of the wound healing process. Fibronectin is involved in multiple steps of the wound healing process. The functional complexity of fibronectin is carried through its protein diversity, which is controlled in part by alternative RNA splicing, a coordinated transcription and RNA processing. From a rabbit model of airway mucosal wound healing, we isolated and cloned an RNA splicing factor, SRp20, that was coexpressed with Fn1 complementary DNA and suppressed in fetal wounds but induced in postnatal wounds. Previous studies revealed a link between the inclusion and/or exclusion of the alternatively spliced Fn1 variants (extra domain A [EDA], extra domain B [EDB], and a variable region [V]) and outcomes of wound repair. Design: Skin and airway mucosal incisional wounds were made in fetal (gestational day 21-23), weanling (4-6 weeks), and adult (Ͼ6 months) rabbits. Tissues (nonwounded and wounded) were collected at 12 hours (all age groups), 24 hours, and 48 hours (weanling only) after wounding. The expression levels of the 3 Fn1 spliced domain (EDA, EDB, and V)-containing messenger RNA (mRNA) species were assessed by real-time polymerase chain reaction. Results: Fn1 spliced variants were either suppressed or showed no change in fetal skin and airway mucosal wounds 12 hours after injury, whereas the spliced mRNAs were induced in postnatal wounds. The augmented molecular activities of Fn1 spliced variants were more prominent in airway mucosal wounds than in skin wounds. Furthermore, the EDA variant was dominantly selected in adult airway mucosal wounds (6-fold increase), which was strikingly different from the adult skin wounds (1-fold). Conclusion: Our study suggests that the age-dependent activation of Fn1-EDA mRNA may play a fundamental role in differentiating fetal wound regeneration from postnatal wound scar formation during the early events of airway mucosal wound healing.

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Expression of Fibronectin Isoforms Bearing the Alternatively Spliced EIIIA, EIIIB, and V Segments in Corneal Alkali Burn and Keratectomy Wound Models in the Rat

Cited by 9 publications

References 23 publications

Fibronectin EIIIA splicing variant: A useful contribution to forensic wounding interval estimation

Fibronectin EIIIA splicing variant: A useful contribution to forensic wounding interval estimation

Light and electron microscopic study of the corneal stroma during the healing process of alkali-induced ulcer

Age-Dependent Differential Expression of Fibronectin Variants in Skin and Airway Mucosal Wounds

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