Expression of Frizzled 2 in the mouse ovary during oestrous cycle

Wang, S. B.; Xing, Baosong; Lü, Yi; Wang, W.; Xu, Yefen

doi:10.1111/j.1439-0396.2009.00927.x

Cited by 8 publications

(14 citation statements)

References 28 publications

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“…Adult female mice were divided (four per group) in proestrus (P), estrus (E), metestrus (M), and diestrus (DI) groups by vaginal smear according to two consecutive estrous cycles (Wang et al, 2010b). Animals were then sacrificed by cervical dislocation.…”

Section: Experimental Designmentioning

confidence: 99%

“…Real-time qPCR analysis was performed to analyze Frizzled 3 mRNA levels as previously described (Wang et al, 2010b). Both optimal annealing temperature and the amplification size of products are shown in Table 1 …”

Section: Quantitative Polymerase Chain Reaction (Qpcr)mentioning

confidence: 99%

“…The ISH procedure was performed as previously described (Wang et al, 2010b). Frizzled 3 RNA localization was determined using a commercial assay kit (Haoyang Inc., Tianjin, China) according to the manufacturer instructions.…”

Section: Ishmentioning

confidence: 99%

“…Recently, Wnt signaling has been implicated in ovarian development, oogenesis, and early embryonic development (Chan et al, 1992;Vainio et al, 1999;Ricken et al, 2002;Boerboom et al, 2005;Hsieh et al, 2002Hsieh et al, , 2005Kimura et al, 2006;Wu et al, 2007;Wang et al, 2009Wang et al, , 2010bLapointe et al, 2012). Vainio et al (1999) found that Wnt 4-deficient mice exhibit sex reversal and a paucity of oocytes in the newborn ovary, while mice null for Frizzled 4 are infertile and exhibit impaired function of the corpus luteum .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Frizzled 2 mRNA has been detected in a high steady state level in the rat ovary by northern analysis (Chan et al, 1992). The Frizzled 2 protein was highly expressed in granulosa cells and the membrane of oocytes during the mouse estrous cycle (Wang et al, 2010b). Frizzled 1 is required for normal female fertility and may act in part to regulate oocyte maturation and cumulus cell function, but it is unlikely to function as the sole ovarian Wnt 4 receptor (Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle

et al. 2016

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ABSTRACT. Frizzled 3 is an important receptor in the Wnt/β-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes. However, the role of this protein during follicular development in the adult ovary is not known. The present study was designed to investigate the expression and localization of Frizzled 3 mRNA and protein during the estrous cycle in the mouse ovary through in situ hybridization (ISH), real-time quantitative polymerase chain reaction, immunohistochemistry and western blot. ISH results showed that in proestrus, high expression of Frizzled 3 was found in the granulosa and stroma with weak levels in the corpus luteum. In estrus and diestrus, the stroma had high Frizzled 3 expression, but levels were low in granulosa cells and corpus luteum. In the metestrus, moderate expression of Frizzled 3 was found in the stroma but low to no expression was found in luteal cells and follicles. The mRNA and protein levels of Frizzled 3 were found to be the highest in proestrus and diestrus compared to estrus and metestrus (P < 0.05), confirming the ISH results. During estrus and diestrus, high Frizzled 3 expression was observed in the stroma and moderate levels in granulosa cells, and during estrus and proestrus, low expression was seen in the oocyte cell membrane. The western blot results further confirmed this change during the estrous cycle. Together, these results indicate that Frizzled 3 is involved in regulating follicular development and oocyte maturation during the estrous cycle.

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Section: Experimental Designmentioning

confidence: 99%

Section: Quantitative Polymerase Chain Reaction (Qpcr)mentioning

confidence: 99%

Section: Ishmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle

et al. 2016

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Lithium induces follicular atresia in rat ovary through a GSK‐3β/β‐catenin dependent mechanism

Mirakhori

Zeynali

Tafreshi

et al. 2013

Molecular Reproduction Devel

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Lithium chloride (LiCl) is a drug used to treat bipolar disorder, but has side effects in the female reproductive system. Although lithium is known to decrease folliculogenesis and induce follicular atresia in rodent ovaries, its cellular and molecular effects in the ovary have not yet been addressed. To investigate these effects, 23-day-old immature female rats were injected with 10 IU pregnant mare serum gonadotropin (PMSG), followed by injections of 250 mg/kg LiCl every 12 hr for four doses. Ovaries were removed 40 and 48 hr after PMSG administration and prepared for histology, immunohistochemistry, Western blotting, and DNA laddering analysis. Our results showed that in the ovaries of LiCl-treated rats, few antral but more atretic follicles were present compared to those of the control rats. The induction of atresia by LiCl was further confirmed by the presence of DNA fragmentation, accompanied by a reduced level of 17β-estradiol in the serum. At the cellular level, lithium significantly decreased the number of proliferating cell nuclear antigen (PCNA)-positive cells and conversely increased the number of TUNEL-positive cells in the granulosa layer of the antral follicles. At the molecular level, lithium increased the level of phosphorylated glycogen synthase kinase-3β, and unexpectedly decreased the expression of active (stabilized) β-catenin. Altogether, our results indicate that lithium disrupts the balance between proliferation and apoptosis in granulosa cells, leading to follicular atresia possibly through the reduction in both the stabilized β-catenin and 17β-estradiol synthesis.

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Analyses stratified by maternal age and recombination further characterize genes associated with maternal nondisjunction of chromosome 21

2021

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Objective In our previous work, we performed the first genome‐wide association study to find genetic risk factors for maternal nondisjunction of chromosome 21. The objective of the current work was to perform stratified analyses of the same dataset to further elucidate potential mechanisms of genetic risk factors. Methods We focused on loci that were statistically significantly associated with maternal nondisjunction based on this same dataset in our previous study and performed stratified association analyses in seven subgroups defined by age and meiotic recombination profile. In each analysis, we contrasted a different subgroup of mothers with the same set of fathers, the mothers serving as cases (phenotype: meiotic nondisjunction of chromosome 21) and the fathers as controls. Results Our stratified analyses identified several genes whose patterns of association are consistent with generalized effects across groups, as well as other genes that are consistent with specific effects in certain groups. Conclusions While our results are epidemiological in nature and cannot conclusively prove mechanisms, we identified a number of patterns that are consistent with specific mechanisms. In many cases those mechanisms are strongly supported by available literature on the associated genes.

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Expression of Frizzled 2 in the mouse ovary during oestrous cycle

Cited by 8 publications

References 28 publications

Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle

Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle

Lithium induces follicular atresia in rat ovary through a GSK‐3β/β‐catenin dependent mechanism

Analyses stratified by maternal age and recombination further characterize genes associated with maternal nondisjunction of chromosome 21

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