Expression of hOGG1 protein during differentiation of HL-60 cells

Abstract

Human 8-oxo-G-DNA glycosylase 1 (hOGG1) is a DNA glycosylase to cleave 8-oxo-7,8-dihydroguanine (8-oxo-G), a mutagenic DNA adduct formed by oxidant stresses. Here, we examined hOGG1 protein expression and repair activity to nick a DNA strand at the site of 8-oxo-G during differentiation of hematopoietic cells using HL-60 cells. Overall expression of hOGG1 protein was increased during granulocytic differentiation of HL-60 cells induced by DMSO and monocytic differentiation by vitamine D 3 . Greater level of hOGG1 protein was expressed in DMSO-treated cells. However, change in the DNA nicking activity was not in parallel with the change in hOGG1 protein expression, especially in PMA-treated cells. In PMAtreated cells, the level of hOGG1 protein was lowered, even though the DNA nicking activity was elevated, in a manner similar to the changes in serumdeprived HL-60 cells. These results indicate that hOGG1 expression change during differentiation of hematopoietic stem cells for adaptation to new environments. And the DNA cleaving activity may require additional factor(s) other than expressed hOGG1 protein, especially in apoptotic cell death.

Acetoaminophen-induced accumulation of 8-oxodeoxyguanosine through reduction of Ogg1 DNA repair enzyme in C6 glioma cells

“…One major immunoreactive band (apparent Mr 38.7 kDa), similar to the size of the rat Ogg1 protein (Prieto Alamo et al, 1998) and human Ogg1 protein (Lee et al, 2003), was detected in the nucleus from untreated control, H2O2-, or DMSO-treated cells ( Figure 5A), along with another minor band (Mr about 33.0 kDa). However, there was little difference in the density of both 38.7 and 33.0 kDa bands among the untreated control, H2O2-, and DMSO-treated cells ( Figure 5A).…”

Comparative analysis of blood lymphocytes of patients with acute and chronic myeloid leukemia by surface and intracellular α1-acid glycoprotein and fibronectin

“…Це може свідчити про підвищення експресії цього білка лімфоцитами за ГМЛ, оскільки розрахунок коефіцієнта коре-ляції Пірсона показав майже функціональну залежність між рівнем АГП усередині та на поверхні лімфоцитів за ГМЛ. Так, за даними (I. H. Lee, 2000), що отримані на клітинній лінії HL-60, яку використовують як модель промієлоцитних лейкемичних клітин, експресія АГП підвищується та є ознакою блокування процесів проліфера-ції, інвазії та метастазування таких клітин [9,10]. Тому можна припустити, що під-вищення рівня внутріклітинного АГП за умов гострого мієлолейкозу є ознакою бло-кування процесів проліферації та метастазування лімфоцитів.…”