A method has been developed for the synthesis of granular polymeric materials with immobilized N-chloro-sulfonamide groups. Commercially available resin polymers widely applied for the preparation of ion exchangers have been used as polymer carriers. The elaborated technological conditions make it possible to modify these resins with a high conversion degree, without deteriorating the strength characteristics, and with the possibility of regulating the concentration of active chlorine over a wide range (up to 11 % w/w). The structure of the synthesized polymers was confirmed by IR spectroscopy data. To determine the concentration of functional groups, a special method of iodometric titration has been developed. The processes of emission of active chlorine from synthesized polymers into aqueous solutions have been studied. It has been shown that this process significantly depends on the composition of the solution: no release of active chlorine into distilled water is observed, and when using tap water, its concentration is reached 5–8 mg/dm3 and remains up to 30 days when the granules are in water. The processes of activation of active chlorine emission by compounds of amine nature have been studied, the corresponding kinetic curves of the dependence between change in the concentration of active chlorine in solution and the used activator are presented. It has been found that the nature of the used activator strongly affects, among other things, the stability of the obtained chlorine-active solutions. Taurine and sulfamic acid are found to be the optimal activators for obtaining stable solutions of active chlorine of high concentration. The composition of the N-chloro-taurin solution obtained in this way has been additionally analyzed by UV spectroscopy. Thus, the synthesized polymers make it possible to quickly in situ obtain high-purity solutions of active chlorine without the use of special electrochemical equipment. The polymers themselves are compact, stable, and can be repeatedly regenerated

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Virucidal properties of N-chlorosulfonamides immobilized on fibrous styrene-divinylbenzene copolymers have been studied. Corresponding materials with different functional group structures and chlorine content have been synthesized on FIBAN polymer carriers in the form of staple fibers and non-woven fabrics. The study has been conducted in general accordance with EN 14476 standard on poliovirus type-1 and adenovirus type-5. It has been found that all tested samples exhibit pronounced virucidal activity: regardless of the carrier polymer form, sodium N-chlorosulfonamides inactivated both viruses in less than 30 s, and N,N-dichlorosulfonamides—in 30–60 s. The main mechanism of action of these materials, obviously, consists in the emission of active chlorine from the functional group into the treated medium under the action of the amino groups of virus fragments and cell culture. Considering the previously described antimicrobial and reparative properties of such materials, as well as their satisfactory physical and mechanical properties, the synthesized polymers are promising for the creation of medical devices with increased resistance to microbial contamination, such as protective masks, filter elements, long-acting wound dressings, and others.

Objective. The aim of the present investigation was to study the expression of genes encoding IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of this protein in intergenic interactions. Methods. Silencing of HSPB8 mRNA was introduced by HSPB8 specific siRNA. The expression level of HSPB8, IRS1, HK2, GLO1, HOMER3, MYL9, NAMPT, PER2, PERP, GADD45A, and DEK genes was studied in U87 glioma cells by quantitative polymerase chain reaction. Results. It was shown that silencing of HSPB8 mRNA by specific to HSPB8 siRNA led to a strong down-regulation of this mRNA and significant modification of the expression of IRS1 and many other genes in glioma cells: strong up-regulated of HOMER3, GLO1, and PERP and down-regulated of MYL9, NAMPT, PER2, GADD45A, and DEK gene expressions. At the same time, no significant changes were detected in the expression of HK2 gene in glioma cells treated by siRNA, specific to HSPB8. Moreover, the silencing of HSPB8 mRNA enhanced the glioma cells proliferation rate. Conclusions. Results of this investigation demonstrated that silencing of HSPB8 mRNA affected the expression of IRS1 gene as well as many other genes encoding tumor growth related proteins. It is possible that the dysregulation of most of the studied genes in glioma cells after silencing of HSPB8 is reflected by a complex of intergenic interactions and that this polyfunctional chaperone is an important factor for the stability of genome function and regulatory mechanisms contributing to the tumorigenesis control.