Expression of hypoxia inducible factor‐1α and vascular endothelia growth factor in vocal polyps

Fang, Rui; Jiang, Jack J.; Smith, Benjamin L.; Wu, Daquan

doi:10.1002/lary.23743

Cited by 11 publications

(11 citation statements)

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“…Scar VFF exhibited the highest IL-8 cytokine expression overall, which warrants further discussion. One would speculate that fibroblasts derived from vocal fold polyps would be more likely to produce higher amounts of IL-8 due to the inflammatory nature of the pathology [ 32 , 33 ], as well as the higher constitutive levels of TLR4 gene and greater percentage of NF-ĸB activated cells that we observed in the current study. These downstream discrepancies suggest different regulatory proteins may be controlling the expression in each cell type, possible mediating the disorder.…”

Section: Discussionmentioning

confidence: 96%

“…Under these pathologic conditions, surrounding cells can undergo damage or stress, as the environment is enriched with inflammatory mediators (i.e. COX-2, HIF-1α) [ 32 , 33 ], proteases (MMP-2 ,-9) [ 33 ], and ECM proteins (i.e. fibronectin, decorin) [ 31 , 34 , 35 ] that can produce endogenous ligands and subsequently activate TLRs (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Lipopolysaccharide responsiveness in vocal fold fibroblasts

2014

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BackgroundVocal fold fibroblast’s (VFF) strategic location in the lamina propria and their ability to respond to external stimuli by producing inflammatory molecules suggest their possible direct involvement in innate immunity. Toll-like receptors (TLRs) are an essential signaling component to this response, as they allow for recognition of various microorganisms, leading to subsequent induction of pro-inflammatory genes. The objective of this study was to elucidate the role of VFF in the host immune response and subsequent influence on inflammatory cytokine secretion.MethodsVFF derived from polyp, scar, and normal tissue were treated with 5 μg/ml lipopolysaccharide (LPS). TLR1 through 9, CD14, and MD-2 were measured during stable conditions by polymerase chain reaction (PCR). Expression of TLR4 and IL-1R type-1 genes were quantified after 24 hrs LPS stimulation by reverse transcription-PCR. LPS responsiveness was determined by NF-κB nuclear translocation as measured by subunit p65 expression in nucleus with immunocytochemistry. Downstream effects were confirmed with immunoassay measuring IL-8 concentrations in supernatant after 8 hrs.ResultsAll VFFs constitutively expressed TLR1 to 6, TLR9, CD14, and MD-2 mRNA. Polyp VFF exhibited significantly higher TLR4 transcript levels (p < 0.001) in comparison to scar and normal VFF. LPS stimulated scar and polyp VFF exhibited increased levels of p65 in the nucleus (p < 0.01) and secreted greater IL-8 protein (p < 0.0001) compared to normal VFF.ConclusionVFF constitutively express genes for the receptors essential to the host immune response. Scar and polyp VFF produced greater LPS responsiveness resulting in over-activated inflammatory patterns. These findings support VFF role in the pathogenesis of inflammatory vocal fold disorders and suggests their presence in the wound bed could lead to chronic inflammation.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide responsiveness in vocal fold fibroblasts

2014

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“…As Fang et al 46 demonstrated HIFs can also be expressed in benign laryngeal neoplasms. Therefore, the diagnostic biomarker role of HIFs is unclear.…”

Section: Discussionmentioning

confidence: 96%

Serum hypoxia‐inducible factor‐2: A candidate prognostic biomarker for laryngeal cancer

Eskıızmır

Koçal

Uysal

et al. 2021

Clinical Otolaryngology

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The twenty-first century is described as the 'age of biomarker discovery' concerning early diagnosis and effective treatment of cancer. 1 However, the biomarker for head and neck cancers (HNCs) is inadequate. Currently, only Human papillomavirus and p16 expression are available for oral and oropharyngeal cancers. 2 Recently, hypoxia-related biomolecules are candidate biomarkers for several cancers.Hypoxia refers to the imbalance between the tissue's demand for oxygen and the supply of oxygen to the tissue. It orchestrates several processes such as tumoural progression and metabolism, chaotic angiogenesis, anoikis resistance and metastasis cascade. [3][4][5][6] Solid tumours are known to have dynamic hypoxic areas with heterogenic

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“…Therefore, the oxidative metabolism was simultaneously detected before and after vocal loading task in this study. According to the result of previous study, hypoxia of vocal cords was induced by vocal overuse 49 . Our results demonstrated the vocal cord hypoxia after vocal loading task both in male and female groups (as shown in Fig.…”

Section: Discussionmentioning

confidence: 96%

A feasibility study on non-invasive oxidative metabolism detection and acoustic assessment of human vocal cords by using optical technique

Lin

Chen

Liu

et al. 2017

Sci Rep

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Voice disorder such as vocal fatigue is a common and complex multifaceted clinical problem that presents a significant impact on quality of life. In this study, the functional near-infrared diffuse optical technique (fNIRS-DOT) was proposed as a novel approach for human vocal cords oxidative metabolism detection and acoustic assessment simultaneously to provide a multidimensional assessment of voice disorder. A totally of 60 healthy subjects included 30 male and 30 female adults of age-matched were recruited and performed a vocal loading task to trigger a mild inflammation of the vocal cords in this study. In the results of oxidative metabolism, the vocal cords expressed hypoxia after vocal loading task in both male and female groups that could provide relevant information on the relationship between tissue oxygen consumption and supply for vocal cords diagnosis. Additionally, the results of optical acoustic assessment revealed the worse/changes voice quality after vocal loading task. Therefore, integration of non-invasive oxidative metabolism detection and acoustic assessment by using optical technique could provide more relevant information for diagnosis of voice disorders. The multi-functional vocal cords detection system could provide a good feasibility for clinical applications such as diagnosis and therapeutic monitoring of voice disorder.

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Expression of hypoxia inducible factor‐1α and vascular endothelia growth factor in vocal polyps

Abstract: The increased expression of HIF-1α in vocal polyps indicates that vocal fold overvibration induced hypoxia may play an important role in the pathogenetic mechanism of vocal polyps.

Cited by 11 publications

References 20 publications

Lipopolysaccharide responsiveness in vocal fold fibroblasts

Lipopolysaccharide responsiveness in vocal fold fibroblasts

Serum hypoxia‐inducible factor‐2: A candidate prognostic biomarker for laryngeal cancer

A feasibility study on non-invasive oxidative metabolism detection and acoustic assessment of human vocal cords by using optical technique

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