Expression of Inhibin a-Subunit in Horse Testis.

Fujimura, Sakie; Hondo, Eiichi; Kobayashi, Tohru; Yamanouchi, Keitaro; Inoue, Noboru; Nagata, S.; Watanabe, Gen; Taya, Kazuyoshi; Kitamura, Nobuo; Yamada, Junzo

doi:10.1292/jvms.60.937

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…The present finding that inhibin ␣ subunit protein and its mRNA are present in Sertoli cells suggest that the cells may secrete inhibin ␣ subunit precursor, inhibin pro-␣C, and agrees with previous studies in other species (Fujimura et al, 1998;Nagata et al, 1998;Roberts et al, 1989;Kishi et al, 2000;Jin et al, 2001). Precursors of inhibin ␣ subunit have been reported to modulate FSH action (Schneyer et al, 1991) and have been found to play an important role in male reproductive physiology (Tuohimaa et al, 1993;Nagata et al, 2000).…”

Section: Concentrations Of Inhibin a Inhibin B And Inhibin Pro-␣c Isupporting

confidence: 93%

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

Wei

Arai

Herath

et al. 2001

Journal of Andrology

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ABSTRACT:The expression of inhibin subunits in the testes of the Göttingen miniature pig was examined by in situ hybridization and immunohistochemistry. In addition, the major forms were determined by enzyme-linked immunosorbent assay (ELISA). Strong positive immunostaining for the inhibin ␣ subunit was observed in Sertoli and late-stage germ cells, but it was weak in Leydig cells. However, Leydig cells showed strong positive staining for the ␤A subunit, but Sertoli cells and spermatogonia showed a weak reaction. Strong positive immunostaining for the ␤B subunit was observed in Leydig cells but spermatogonia showed weak staining for it. In contrast to the staining specificity of inhibin ␣ and ␤A subunits, the ␤B subunit did not exhibit positive staining in Sertoli cells. In situ hybridization revealed that although the ␣ subunit mRNA signal was highly expressed in all cell types, the reaction appeared to be stronger in Sertoli cells and spermatogonia than in Leydig cells. ␤A subunit mRNA expression was somewhat identical to that of the ␣ subunit, however, germ cells showed a weak stain for it. A strong, positive mRNA signal for the ␤B subunit was confined to Leydig cells and late-stage germ cells. ELISA results showed that concentrations of inhibin B and inhibin pro-␣C were high in the circulation and testes. In contrast, inhibin A levels in both plasma and testes were undetectable. The present results strongly suggest that inhibin B is the major form of circulating inhibin and that Leydig cells are the predominant source of this dimeric inhibin in male Göttingen miniature pigs. Furthermore, the germ cells also appear to be an important source of circulating inhibins.

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Section: Concentrations Of Inhibin a Inhibin B And Inhibin Pro-␣c Isupporting

confidence: 93%

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

Wei

Arai

Herath

et al. 2001

Journal of Andrology

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“…Furthermore, the expression of inhibin α-subunit mRNA by in situ hybridization was also observed in these cells in the stallion [18]. Changes in circulating concentrations of ir-inhibin and inhibin pro-αC in the present study had a strong positive correlation with each other.…”

Section: Discussionsupporting

confidence: 70%

Inhibin Pro-.ALPHA.C as the Marker of Testicular Function in the Stallion.

Nagata

Nagaoka

Shinbo

et al. 2000

Journal of Reproduction and Development

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Abstract. Inhibin pro-αC is a precursor form of inhibin α-subunit. A large amount of inhibin pro-αC is secreted from testes in male animals, and it is considered to have an important role in the physiology of male reproduction. The purpose of this study was to determine the relationship between inhibin pro-αC and reproductive ability in the mature stallion. Peripheral blood samples were collected from normal stallions and anabolic steroid (nandrolone decanoate) treated stallions. Plasma concentrations of inhibin pro-αC were measured by an enzyme linked immunosorbent assay system. Circulating concentrations of inhibin pro-αC in normal stallions showed clear seasonal changes and the highest concentrations were observed in the breeding season (from March to June). After treatment with anabolic steroid, peripheral inhibin pro-αC levels rapidly decreased and reached significantly lower levels compared to the pre-treatment value (p<0.05). The variations of circulating inhibin pro-αC showed a high positive correlation with other testicular hormones. These results clearly demonstrate that circulating concentrations of inhibin pro-αC reflect spermatogenic ability due to seasonal change or treatment with anabolic steroid in the stallion, and it may be a useful new marker for testicular functions.

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“…Inhibin (INH) in male animals is a glycoprotein produced by Sertoli cells [23,24,28] and is composed of two subunits, an α-unit and a β-unit [8,23]. The bioactivity of INH-α is important for spermatogenesis in the testis [11,24,29] and is especially correlated with Sertoli cell function [19,21]. The blood plasma INH concentration is lower in cryptorchid men than in normal men [3,7,21], and the rate of occurrence of SCT has been found to be markedly higher in knockout mice with INH-α gene deficiency [4].…”

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confidence: 99%

Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

et al. 2007

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ABSTRACT. The proportions of Sertoli cell tumor (SCT), seminoma and Leydig cell tumor in 50 dogs with unilateral testicular tumors were 52%, 36% and 12%, respectively. The rate of occurrence of SCT in the cryptorchid testis was very high (71%). The testicular superoxide dismutase (SOD) activity, testicular heat shock protein (HSP) 70 concentration and peripheral blood plasma inhibin (INH)-α concentration of 10 dogs with a unilateral cryptorchid testis and no testicular tumors, 10 dogs with SCT in a unilateral cryptorchid testis and 10 normal dogs, all aged 5-15 years, were measured in order to identify high risk factors for the occurrence of SCT in the canine cryptorchid testis. The mean SOD activity in cryptorchid testes and SCTs was significantly lower and higher, respectively, than in normal testes (both P<0.01). The mean HSP 70 concentration in both cryptorchid testes and SCTs was significantly higher than in normal testes (both P<0.01). The mean plasma INH-α concentration of the cryptorchid and SCT dogs was significantly lower and higher, respectively, than in normal dogs (P<0.05 and 0.01, respectively). The low SOD activity in the cryptorchid testis, low blood plasma INH-α concentration of the cryptorchid dogs and high HSP 70 concentration in the SCTs may be related to the occurrence of SCT and tumor cell proliferation in canine cryptorchid testes.

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Expression of Inhibin a-Subunit in Horse Testis.

Cited by 11 publications

References 40 publications

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

Inhibin Pro-.ALPHA.C as the Marker of Testicular Function in the Stallion.

Testicular Superoxide Dismutase Activity, Heat Shock Protein 70 Concentration and Blood Plasma Inhibin-.ALPHA. Concentration of Dogs with a Sertoli Cell Tumor in a Unilateral Cryptorchid Testis

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