Expression of LDL receptor and uptake of LDL in mouse preimplantation embryos

Sato, Naoki; Kawamura, Kazuhiro; Fukuda, Jun; Honda, Yoshiyuki; Sato, Tutomu; Tanikawa, Hideo; Kodama, Hiroyuki; Tanaka, Toshinobu

doi:10.1016/s0303-7207(03)00082-0

Cited by 23 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lysophosphatidic acid stimulates cavitation of embryo [Kobayashi et al, 1994] and accelerates blastocyst differentiation [Liu and Randall, 2004]. Mouse blastocysts take up low-density lipoprotein and may metabolize it to produce cholesterol for steroid biosynthesis [Sato et al, 2003]. These reports warrant further investigation on the role of PLTP in embryo development.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct

Lee

Kwok

Chung

et al. 2005

J of Cellular Biochemistry

View full text Add to dashboard Cite

In mammal, fertilization and early preimplantation embryo development occurs in the oviduct. Evidence is accumulating that the oviductal epithelia secrete various biomolecules to the lumen during the secretory phase of the estrus cycle to enhance embryo development. This secretory activity of the oviduct is under the regulation of steroid hormones. Observations also suggested that the gametes and embryos modulate the physiology and gene-expressing pattern of the oviduct. However, the underlying molecular changes remain elusive. We hypothesize that the developing embryos interact with the surrounding environment and affect the gene expression patterns of the oviduct, thereby modulating the oviductal secretory activity conducive to the preimplantation embryo development. To test this hypothesis, suppression subtractive hybridization (SSH) was used to compare the gene expressions in mouse oviduct containing transferred in vitro cultured preimplantation embryos with that of oviduct containing oocytes during the preimplantation period. We reported here the identification and characterization of phospholipids transfer protein (PLTP), which is highly expressed in the embryo-containing oviduct and localized at the oviductal epithelium by in situ hybridization. PLTP contains signal peptide putative for secretory function. More importantly, PLTP mRNA increases in the oviductal epithelia of pregnant, but not pseudo-pregnant mice when assayed by real-time PCR. Taken together, our data suggested that PLTP may play important role(s) during in vivo preimplantation embryo development. This molecule would be a target to delineate the mechanisms and the roles of oviductal secretory proteins on early embryonic development.

show abstract

Section: Discussionmentioning

confidence: 99%

Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct

Lee

Kwok

Chung

et al. 2005

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Consistently, acetate is inefficiently converted to cholesterol in granulosa-free oocytes (Su et al 2008). Oocytes also lack the ability to import cholesterol as they do not express receptors for HDL cholesterol and LDL cholesterol (Sato et al 2003;Trigatti et al 1999). Oocytes are thus deficient in both cholesterol synthesis and uptake of extracellular cholesterol.…”

Section: Metabolic Supportmentioning

confidence: 99%

Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche

El‐Hayek

Clarke

2016

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

In the mammalian ovary, each oocyte grows and develops within its own structural and developmental niche-the follicle. Together with the female germ cell in the follicle are somatic granulosa cells, specialized companion cells that surround the oocyte and provide support to it, and an outer layer of thecal cells that serve crucial roles including steroid synthesis. These follicular compartments function as a single physiological unit whose purpose is to produce a healthy egg, which upon ovulation can be fertilized and give rise to a healthy embryo, thus enabling the female germ cell to fulfill its reproductive potential. Beginning from the initial stage of follicle formation and until terminal differentiation at ovulation, oocyte and follicle growth depend absolutely on cooperation between the different cellular compartments. This cooperation synchronizes the initiation of oocyte growth with follicle activation. During growth, it enables metabolic support for the follicle-enclosed oocyte and allows the follicle to fulfill its steroidogenic potential. Near the end of the growth period, intra-follicular interactions prevent the precocious meiotic resumption of the oocyte and ensure its nuclear differentiation. Finally, cooperation enables the events of ovulation, including meiotic maturation of the oocyte and expansion of the cumulus granulosa cells. In this chapter, we discuss the cellular interactions that enable the growing follicle to produce a healthy oocyte, focusing on the communication between the germ cell and the surrounding granulosa cells.

show abstract

“…SCARB1, scavenger receptor class B, member 1, also known as SR-BI) or LDL-cholesterol (i.e. LDLR) are not expressed by mouse oocytes (Sato et al, 2003;Trigatti et al, 1999) suggesting that mouse oocytes are unable to take up carrier-borne cholesterol. What is the source of oocyte cholesterol needed for oocyte development and subsequent embryogenesis?…”

Section: Research Articlementioning

confidence: 99%

Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells

et al. 2008

View full text Add to dashboard Cite

*Oocyte-derived bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are key regulators of follicular development. Here we show that these factors control cumulus cell metabolism, particularly glycolysis and cholesterol biosynthesis before the preovulatory surge of luteinizing hormone. Transcripts encoding enzymes for cholesterol biosynthesis were downregulated in both Bmp15-/-and Bmp15 -/-Gdf9 +/-double mutant cumulus cells, and in wild-type cumulus cells after removal of oocytes from cumulus-cell-oocyte complexes. Similarly, cholesterol synthesized de novo was reduced in these cumulus cells. This indicates that oocytes regulate cumulus cell cholesterol biosynthesis by promoting the expression of relevant transcripts. Furthermore, in wild-type mice, Mvk, Pmvk, Fdps, Sqle, Cyp51, Sc4mol and Ebp, which encode enzymes required for cholesterol synthesis, were highly expressed in cumulus cells compared with oocytes; and oocytes, in the absence of the surrounding cumulus cells, synthesized barely detectable levels of cholesterol. Furthermore, coincident with reduced cholesterol synthesis in double mutant cumulus cells, lower levels were also detected in cumulus-cell-enclosed double mutant oocytes compared with wild-type oocytes. Levels of cholesterol synthesis in double mutant cumulus cells and oocytes were partially restored by co-culturing with wild-type oocytes. Together, these results indicate that mouse oocytes are deficient in synthesizing cholesterol and require cumulus cells to provide products of the cholesterol biosynthetic pathway. Therefore, oocyte-derived paracrine factors, particularly, BMP15 and GDF9, promote cholesterol biosynthesis in cumulus cells, probably as compensation for oocyte deficiencies in cholesterol production.

show abstract

Expression of LDL receptor and uptake of LDL in mouse preimplantation embryos

Cited by 23 publications

References 19 publications

Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct

Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct

Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche

Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells

Contact Info

Product

Resources

About