Expression of lncRNA TINCR in the placenta of patients with pre-eclampsia and its effect on the biological behaviours of trophoblasts

Abstract

Summary To explore the effect of lncRNA TINCR on the biological behaviours of trophoblasts, we detected and analyzed the expression of terminal differentiation-induced non-protein coding RNA (TINCR) in the placenta tissues of pre-eclamptic and non-pre-eclamptic pregnant women. The gain- and loss-of-function of TINCR was performed to examine the proliferation, migration and invasion abilities of Htr-8/Svneo cells. The levels of epithelial–mesenchymal transition (EMT)-related proteins, cyclin and Wnt/β-catenin pathway were detected. High expression of lncRNA TINCR appeared in placental tissues of patients with pre-eclampsia. The proliferation, invasion and migration of Htr-8/Svneo cells were promoted by TINCR downregulation; the cells were transited from G0/G1 to S phase; and EMT was promoted and the Wnt/β-catenin pathway was activated. In summary, the downregulation of lncRNA TINCR activated the Wnt/β-catenin pathway and promoted the proliferation, invasion and migration of Htr-8/Svneo cells. This study may provide a theoretical basis for treatment of patients with pre-eclampsia.

Interaction of lncRNA Gm2044 and EEF2 promotes estradiol synthesis in ovarian follicular granulosa cells

“…The functions and molecular mechanisms of lncRNA in female reproduction have been reported at present [ 1 , 2 ]. Decreased expression of lncRNA TINCR in the placentas of pre-eclampsia patients can enhance cellular proliferation, migration, and invasion by promoting the Wnt/β-catenin signaling pathway [ 1 ].…”

“…The functions and molecular mechanisms of lncRNA in female reproduction have been reported at present [ 1 , 2 ]. Decreased expression of lncRNA TINCR in the placentas of pre-eclampsia patients can enhance cellular proliferation, migration, and invasion by promoting the Wnt/β-catenin signaling pathway [ 1 ]. LncRNA AFAP1-AS1 coordinates with EZH2 to repress Dusp5 transcription by modifying H3K27m3 of the Dusp5 promoter and then promotes proliferation, migration, and invasion of trophoblast cells [ 2 ].…”

Gene Expression Network Analysis Identifies Potential Targets for Prevention of Preeclampsia

“… 20 At the molecular level, the microRNA spectrum of blood exosomes has been used to evaluate the pathophysiology of preeclampsia and predict the disease, 21 and there are more and more studies at gene level. 22–25 …”

“…20 At the molecular level, the microRNA spectrum of blood exosomes has been used to evaluate the pathophysiology of preeclampsia and predict the disease, 21 and there are more and more studies at gene level. [22][23][24][25] Zhang and Horvath 26 conducted the first WGCNA analysis in 2005 and then published WGCNA R software package in R based on theory of the research method in 2008. As an efficient and accurate bioinformatics approach, WGCNA has been widely used for the gene network analysis.…”