Expression of matrix metalloproteinases and ovarian morphological changes in androgenized cyclic female guinea pigs

Li, Junrong; Shen, Ting; Wang, Yanli; Wei, Quanwei

doi:10.1016/j.tice.2015.10.003

Cited by 3 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteolytic enzymes, classified into five groups: gelatinases (MMP-2 and MMP-9), mainly targeting denatured interstitial collagen (gelatin) and type I and IV collagen fibers; stromelysins (MMP-3, MMP-10, and MMP-11), targeting non-collagen molecules and proteoglycans; collagenases (MMP-1, MMP-8, MMP-13, and MMP-18), targeting fibrillar forms of collagen; matrilysins (MMP-7 and MMP-26) and MT-MMPs (MMP-14, MMP-15, MMP-16, MMP-17, MMP-24, and MMP-25), a group of transmembrane enzymes not only cleaving ECM components, but also activating other MMPs [9][10][11][12]. MMPs are implicated in remodeling of many connective tissues in physiological and pathological processes such as reproduction, embryonic development, morphogenesis, angiogenesis and fibrosis of such organs as liver, kidney and pulmonary [13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The role of homocysteine in seminal vesicles remodeling in rat

Ghoul¹,

Moudilou²,

Cherifi³

et al. 2017

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

The role of homocysteine in seminal vesicles remodeling in rat

Ghoul¹,

Moudilou²,

Cherifi³

et al. 2017

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…The modest changes observed in the MMP system with increased MMP9 protein in theca cells from large preantral follicles and stroma and reduction in TIMP1 and matrix proteins LAMB and collagen are suggestive of moderate increase in ovarian MMP activity in prenatal T-treated females. An increase in MMP2 and 9 protein expression has also been reported in atretic cystic follicles of hyperandrogenic guinea pig 60 and atretic follicles of hypophysectomized sheep. 61 These findings are not comparable to the findings in the present study, as expression of MMP members was assessed only in healthy follicles.…”

Section: Impact Of Prenatal T Excessmentioning

confidence: 70%

Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins

et al. 2018

View full text Add to dashboard Cite

Prenatal testosterone (T)-treated sheep, similar to women with polycystic ovary syndrome (PCOS), manifests reproductive defects that include multifollicular ovarian phenotype. Women with PCOS manifest increased ovarian matrix metalloproteinases (MMPs) activity. We tested the hypothesis that gestational T excess in sheep would alter ovarian expression of MMPs, tissue inhibitors of MMP (TIMP) and their target proteins laminin B (LAMB), collagen, tumor necrosis factor alpha (TNF), and connexin 43 (GJA1) consistent with increased MMP activity and that these changes are developmentally regulated. The ovarian content of these proteins was quantified by immunohistochemistry in fetal day 90, 140, and adult (21 months of age) ovaries. Prenatal T excess lowered GJA1 protein content in stroma and granulosa cells of primary follicles from fetal day 90 ovaries and decreased stromal MMP9, TIMP1, and LAMB in fetal day 140 ovaries. In the adult, prenatal T-treatment (1) increased MMP9 in theca cells of large preantral follicles and stroma, TNF in granulosa cells of small and large preantral follicles and theca cells of large preantral and antral follicles, and GJA1 in stroma, theca cells of large preantral follicles, and granulosa cells of antral follicles and (2) reduced TIMP1 in stroma, theca cells of large preantral and antral follicles, LAMB in stroma and small prenatral follicles, and collagen content in stroma and around antral follicles. These findings suggest a net increase in MMP activity and its target proteins TNF and GJA1 in prenatal T-treated adult but not in fetal ovaries and their potential involvement in the development of multifollicular morphology.

show abstract

Distinctions in PCOS Induced by Letrozole Vs Dehydroepiandrosterone With High-fat Diet in Mouse Model

et al. 2022

View full text Add to dashboard Cite

Polycystic ovarian syndrome (PCOS) is a complex health condition associated with metabolic disturbances and infertility. Recent data suggest that the prevalence of PCOS is increasing among women globally, although the etiology of these trends is undefined. Consequently, preclinical models that better reflect the biology of PCOS are urgently needed to facilitate research that can lead to the discovery of prevention strategies or improved management. The existing animal models have several limitations as they do not reflect all the PCOS features metabolically and/or phenotypically. Therefore, there is no clear consensus on the use of appropriate animal model and selection of the most appropriate PCOS-inducing agent. To that end, we have established a Swiss albino mouse model of PCOS based on 3 weeks daily treatment with letrozole (50 μg/day; intraperitoneal) and dehydroepiandrosterone (DHEA, 6 mg/100 g body weight; subcutaneous) in 5-week old female mice fed on normal or high-fat diet (HFD). Mice were regularly assessed for body weight, blood glucose and estrous cycle. Three weeks post-drug administration, mice were sacrificed and assessed for blood-based metabolic parameters as well as ovarian function. Our results indicate that dehydroepiandrosterone combined with high-fat diet produces changes mimicking those of clinical PCOS including elevated serum testosterone and luteinizing hormone, dyslipidemia, poor ovarian microenvironment and development of multiple ovarian cysts recapitulating cardinal features of PCOS. In comparison, normal diet and/or letrozole produced fewer features of PCOS. The data from the experimental models presented here can improve our understanding of growing concern over women’s health caused by PCOS.

show abstract

Expression of matrix metalloproteinases and ovarian morphological changes in androgenized cyclic female guinea pigs

Cited by 3 publications

References 18 publications

The role of homocysteine in seminal vesicles remodeling in rat

The role of homocysteine in seminal vesicles remodeling in rat

Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins

Distinctions in PCOS Induced by Letrozole Vs Dehydroepiandrosterone With High-fat Diet in Mouse Model

Contact Info

Product

Resources

About