Expression of myosin heavy chain isoforms in regenerating myotubes of innervated and denervated chicken pectoral muscle

Cerny, Lisbeth C.; Bandman, Everett

doi:10.1016/0012-1606(87)90040-6

Cited by 101 publications

(55 citation statements)

References 52 publications

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“…In the early events, fast isoforms were predominantly expressed in both the soleus and EDL muscles. This is in agreement to what has been reported previously (8,11,16,19). However, after 28 days of regeneration, slow (I) MyHC was found to be expressed in both slow-and fast-twitch muscles.…”

Section: Discussionsupporting

confidence: 93%

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Launay¹,

Noirez²,

Butler-Browne³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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Launay, Thierry, Philippe Noirez, Gillian Butler-Browne, and Onnik Agbulut. Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity. Am J Physiol Regul Integr Comp Physiol 290: R1508 -R1514, 2006. First published January 19, 2006 doi:10.1152/ajpregu.00486.2005.-In the literature, there is an ambiguity as to the respective roles played by calcineurin phosphatase activity (CPA) and muscle innervation in the reestablishment of the slow-twitch muscle phenotype after muscle regeneration in different species. In this study, we wanted to determine the role of calcineurin and muscle innervation on the appearance and maintenance of the slow phenotype during mouse muscle regeneration. The pattern of myosin expression and CPA was analyzed in adult (n ϭ 15), regenerating (n ϭ 45) and denervated-regenerating (n ϭ 32) slow-twitch soleus and fast-twitch extensor digitorum longus (EDL) muscles. Moreover, in a second group of denervated-regenerating mice (n ϭ 9), the animals were treated with a calcineurin inhibitor. Regeneration was induced by injection of cardiotoxin and in the denervatedregenerating group, denervation was carried out by cutting the sciatic nerve before the administration of cardiotoxin. In innervated-regenerating soleus muscle, CPA increased continuously after 10 days postinjury and by 21 days, there was a 3.5-fold increase in CPA compared with adult basal level, whereas in innervated-regenerating EDL muscle, CPA remained unchanged. Moreover, our results show that in denervated-regenerating muscles, the MyHC profile was identical in spite of the functional differences inherent in these muscles. In long-term denervated-regenerating muscles, a slow muscle phenotype was reexpressed both in the presence or absence of calcineurin inhibitor. Our results show that although in innervated-regenerating mouse muscle, the appearance of a slow phenotype is correlated with a peak of CPA, in denervated-regenerating muscles, a slow phenotype is triggered and maintained in a calcineurin-and nerve-independent manner. myosin heavy chain; cardiotoxin; denervation; nerve injury; immunohistochemistry

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Section: Discussionsupporting

confidence: 93%

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Launay¹,

Noirez²,

Butler-Browne³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Embryonic/adult fast MHC was detected using EB165 monoclonal antibody (MAb), which reacts with all embryonic MHC isoforms and adult fast MHC (Cerny and Bandman 1987;Bandman and Bennett 1988). EB165 MAb was a kind gift from Everett Bandman (University of California; Davis, CA).…”

Section: Differentiationmentioning

confidence: 99%

Progenitor Cell Isolation from Muscle-derived Cells based on Adhesion Properties

Rouger

Fornasari

Armengol

et al. 2007

J Histochem Cytochem.

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Adult skeletal muscle possesses remarkable regenerative capacity that has conventionally been attributed to the satellite cells. These precursor cells were thought to contain distinct populations with varying myogenic potential. Recently, the identification of multipotent stem cells capable of new myofiber formation has expanded the general view on the muscle regenerative process. Here we examined the characteristics of turkey skeletal muscle-derived cell (MDC) populations that were separated according to their adhesion abilities. We sought to determine whether these abilities could be a potential tool for separating cells with different myogenic commitment. Using the preplate technique, we showed that MDCs display a wide range of adhesion ability, allowing us to isolate a marginal fraction with initial adhesion defect. Methodological investigations revealed that this defect represents an intrinsic and well-established biological feature for these cells. In vitro behavioral and morphological analyses showed that late adherent cells (LACs) share several primitive cell characteristics. Phenotypic assessment indicated that LACs contain early stage myogenic cells and immature progenitors of satellite cells, whereas early adherent cells consist mainly of fully committed precursors. Overall, our findings demonstrate for the first time in an avian model that differential MDC adhesion properties could be used to efficiently purify cells with varying myogenic commitment, including immature progenitor cells. This manuscript contains online supplemental material at http://www.jhc.org . Please visit this article online to view these materials.

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“…Isoform transitions in birds require the up-and downregulation of individual slow MyHC genes in precise sequence (Crow and Stockdale, 1986;Cerny and Bandman, 1987). Although work has elucidated some of the factors regulating MyHC gene expression, including thyroid hormone (Gustafson et al, 1986;Izumo et al, 1986), innervation (Pette, 2001) and activity (Cerny and Bandman, 1986;Kennedy et al, 1986), exactly how these and other mechanisms interact to regulate a complex series of isoform changes is not known.…”

Section: Differentiation and Maturation Of Myotomal Fibersmentioning

confidence: 99%

Regulation of myosin expression during myotome formation

et al. 2003

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The first skeletal muscle fibers to form in vertebrate embryos appear in the somitic myotome. PCR analysis and in situ hybridization with isoform-specific probes reveal differences in the temporal appearance and spatial distribution of fast and slow myosin heavy chainmRNA transcripts within myotomal fibers. Embryonic fast myosin heavy chain was the first isoform expressed, followed rapidly by slow myosin heavy chains 1 and 3, with slow myosin heavy chain 2 appearing several hours later. Neonatal fast myosin heavy chain is not expressed in myotomal fibers. Although transcripts of embryonic fast myosin heavy chain were always distributed throughout the length of myotomal fibers, the mRNA for each slow myosin heavy chain isoform was initially restricted to the centrally located myotomal fiber nuclei. As development proceeded, slow myosin heavy chain transcripts spread throughout the length of myotomal fibers in order of their appearance. Explants of segments from embryos containing neural tube, notochord and somites 7-10, when incubated overnight, become innervated by motor neurons from the neural tube and express all four myosin heavy chain genes. Removal of the neural tube and/or notochord from explants prior to incubation or addition of d-tubocurare to intact explants prevented expression of slow myosin chain 2 but expression of genes encoding the other myosin heavy chain isoforms was unaffected. Thus, expression of slow myosin heavy chain 2 is dependent on functional innervation, whereas expression of embryonic fast and slow myosin heavy chain 1 and 3are innervation independent. Implantation of sonic-hedgehog-soaked beads in vivo increased the accumulation of both fast and slow myosin heavy chain transcripts, as well as overall myotome size and individual fiber size, but had no effect on myotomal fiber phenotype. Transcripts encoding embryonic fast myosin heavy chain first appear ventrolaterally in the myotome, whereas slow myosin heavy chain transcripts first appear in fibers positioned midway between the ventrolateral and dorsomedial lips of the myotome. Therefore, models of epaxial myotome formation must account for the positioning of the oldest fibers in the more ventral-lateral region of the myotome and the youngest fibers in the dorsomedial region.

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Expression of myosin heavy chain isoforms in regenerating myotubes of innervated and denervated chicken pectoral muscle

Cited by 101 publications

References 52 publications

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Progenitor Cell Isolation from Muscle-derived Cells based on Adhesion Properties

Regulation of myosin expression during myotome formation

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