2009
DOI: 10.1186/1742-2094-6-24
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Expression of P2 nucleotide receptors varies with age and sex in murine brain microglia

Abstract: Microglia are implicated in multiple neurodegenerative disorders, many of which display sexual dimorphisms and have symptom onsets at different ages. P2 purinergic receptors are critical for regulating various microglial functions, but little is known about how their expression varies with age or sex. Therefore, comprehensive information about purinergic receptor expression in normal microglia, in both sexes, over age is necessary if we are to better understand their roles in the healthy and diseased CNS. We a… Show more

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Cited by 104 publications
(119 citation statements)
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“…We then determined if P2Y 4 receptors were expressed in microglia. Previous studies had identified that microglia express mRNAs encoding P2Y 4 receptors (19,21,22). We confirmed the expression of the P2Y 4 receptor at the protein level by Western blotting (Fig.…”
Section: Nucleotides Evoke Pinocytosis In Microgliasupporting
confidence: 82%
“…We then determined if P2Y 4 receptors were expressed in microglia. Previous studies had identified that microglia express mRNAs encoding P2Y 4 receptors (19,21,22). We confirmed the expression of the P2Y 4 receptor at the protein level by Western blotting (Fig.…”
Section: Nucleotides Evoke Pinocytosis In Microgliasupporting
confidence: 82%
“…The upregulation of microglial P2X 4 , P2X 7 , and P2Y 6 receptors was also observed in superoxide dismutase 1 mutant expressing animals (these being an accepted model for amyotrophic lateral sclerosis) (185). Changes in the P2X/P2Y mRNA expression were also observed in microglial cultures prepared from animals of different (1-12 mo) age (179).…”
Section: Plasticity Of the Microglial Purinoceptive Systemmentioning
confidence: 78%
“…RNA isolation and qRT-PCR were performed as we described in detail previously (Crain et al 2009; Nikodemova and Watters 2011). Total RNA was extracted from isolated CD11b + cells as well as from microglia-free homogenates (see above) using Tri-reagent (Sigma, MO, USA).…”
Section: Methodsmentioning
confidence: 99%