Expression of P450 aromatase and 17β-hydroxysteroid dehydrogenase type 1 at fetal–maternal interface during tubal pregnancy

Li, Yan; Qin, Li; Zhi, Xu; Wang, Yan Ling; Leng, Jin Hua; Lang, Jing He; Isomaa, Veli; Piao, Yun

doi:10.1016/j.jsbmb.2003.09.013

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…These findings were not unique to Rana dybowskii , as similar results were found in the oviduct of the northern leopard frog, Rana pipiens , which showed that androgen was aromatized to estrogen by the oviduct and suggested that this conversion might account for its growth-promoting effect [ 25 ]. Similar phenomenon was also observed in the epithelial cells of the fimbriae during tubal pregnancy of human compared to normal fallopian tube specimens, which may result in a sufficient local estrogen supply for the maintenance of tubal pregnancy [ 26 ]. In addition, in bovine oviduct primary cell culture system, it was reported that the cell line maintains some important characteristics of the primary cells such as the expression of estrogen receptors and P450arom, showing that androgen can be aromatized in the oviduct to local estrogens [ 27 ].…”

Section: Discussionsupporting

confidence: 55%

Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation

Weng

Liu

et al. 2015

International Journal of Endocrinology

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One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.

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Section: Discussionsupporting

confidence: 55%

Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation

Weng

Liu

et al. 2015

International Journal of Endocrinology

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“…However, the fetomaternal compartment removed from tubal pregnant patients often possesses an integrated fetomaternal interface, as shown in our recent studies (Li et al 2003, Qin et al 2003a. Moreover, tubal pregnancy triggers normal immunoreactivity and hormonal activation of the maternal body (Earl et al 1986, Randall et al 1987, Marx et al 1999, as well as giving rise to immunologically normal and hormonally active trophoblast cells (Vassiliadou & Bulmer 1998, Li et al 2003, Qin et al 2003a. Therefore, tubal pregnancy may provide a unique model to study the mechanism of implantation, particularly from the aspect of trophoblast cells.…”

Section: Introductionmentioning

confidence: 86%

Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during tubal pregnancy

Bai

Wang²,

Qin³

et al. 2005

Reproduction

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Matrix metalloproteinases (MMPs) are responsible for extracellular matrix (ECM) degradation, and their functions are regulated by tissue inhibitors of MMPs (TIMPs). The evidence for the roles of MMPs and TIMPs in implantation and placentation has remained insufficient in humans, especially during the early stages. Tubal pregnancy has some similarities to normal intrauterine pregnancy and therefore may provide a unique model for implantation studies. In the present study, the expression of MMP-2, -9 and -14, and TIMP-1, -2 and -3 at the feto -maternal interface during tubal pregnancy was examined by immunohistochemistry and in situ hybridization. We found that MMP-9 and TIMP-1, -2 and -3 are produced by all types of extravillous cytotrophoblast (EVCT) cells, while MMP-2 and -14 mainly exist in distal column cytotrophoblast (CCT) cells and invasive EVCT cells. Meanwhile, the intensity of MMP-14 and TIMP-1 and -2 increased along the invasive pathway toward maternal interstitium. In addition, MMP-2, -9 and -14 and TIMP-1, -2 and -3 were all detected in the villous CT (VCT) cells. Furthermore, both the mRNA level and immunoreactivity of MMP-9, TIMP-1 and -3 increased, while those of TIMP-2 decreased concurrent with the progression of pregnancy during weeks 3-9. The unique expression pattern of various MMPs and TIMPs at the feto-maternal interface suggests that they may have roles in regulating the controlled invasion of trophoblasts during implantation and placentation. Meanwhile, the study provides a better understanding of the mechanisms involved in cellular events during human pregnancy, especially at the initiation stage of implantation.

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“…Our data and other data show that tubal pregnancy triggers normal immunoreactivity and hormonal activation of the maternal body (Earl et al 1986;Randall et al 1987;Marx et al 1999), as well as giving rise to immunologically normal and hormonally active trophoblast cells (Vassiliadou and Bulmer 1998;Li et al 2003;Qin et al 2003;Bai et al 2005). However, owing to the restricted availability of human specimens with intact fetomaternal interface, much remains unclear concerning the expression patterns of the relevant molecules involved in the interaction between trophoblasts and the maternal endometrium, especially at the very early stage of embryonic implantation.…”

mentioning

confidence: 64%

Expression and Localization of SWAP-70 in Human Fetomaternal Interface and Placenta During Tubal Pregnancy and Normal Placentation

Liu

Dong

Cao

et al. 2007

J Histochem Cytochem.

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SWAP-70 has been demonstrated as a multiple functional signaling protein involved in formation of membrane ruffling induced by signal cascade of tyrosine kinase growth factor receptors. In the present study, the spatial and temporal expression pattern of SWAP-70 on human fetomaternal interface was investigated using specimens collected from tubal and normal pregnancies by in situ hybridization, immunohistochemistry, and Western blotting. Data showed an intense expression of SWAP-70 in trophoblasts at weeks 3-6 of fallopian implantation and at weeks 6-7 of normal pregnancy. The most intense expression was exhibited by those highly motile and invasive extravillous trophoblasts. From gestational week 8 on, the level of SWAP-70 in trophoblasts decreased significantly, and the signal was restricted in villous cytotrophoblast cells. In the in vitro cultured human trophoblast cell line, B6Tert-1, colocalization of SWAP-70 with F-actin was verified. Data in human placenta were similar to what we recently reported on rhesus monkey fetomaternal interface. Our results suggest that SWAP-70 may be involved in regulating migration and invasion of trophoblast cells during the processes of embryonic implantation and placentation in primates.

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Expression of P450 aromatase and 17β-hydroxysteroid dehydrogenase type 1 at fetal–maternal interface during tubal pregnancy

Cited by 21 publications

References 26 publications

Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation

Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation

Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during tubal pregnancy

Expression and Localization of SWAP-70 in Human Fetomaternal Interface and Placenta During Tubal Pregnancy and Normal Placentation

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