Expression of p53 in imprint smears of endometrial carcinoma

Abstract

The aims of this study were to determine the expression of p53 protein in endometrial adenocarcinomas (as a potential prognostic indicator before treatment) as well as normal endometrium in imprint smears and to correlate the results with clinicopathologic parameters of primary untreated endometrial cancer patients. Two hundred fifty five patients were evaluated with endometrial imprint cytology during a 29-month period. Endometrial samples freshly resected from women who underwent total abdominal hysterectomy were studied. One hundred twenty six patients had endometrial carcinoma and 129 cases were diagnosed as normal endometrium. The expression of p53 was assessed by immunocytochemistry. Positive staining was correlated with increased surgical-pathological stage, histological grade and lymph node metastases. High expression of p53 staining was significantly more frequent in histological type II than type I endometrial adenocarcinoma. High-grade endometrial carcinoma had higher proportions and stronger intensity compared with low-grade carcinoma. Negative immunostain for p53 protein was found in proliferative, secretory, and atrophic endometrium. Immunocytochemical findings from p53 stain, in addition to cytomorphologic features, appeared to be useful in the diagnosis and in the postoperative prognosis of endometrial carcinoma in endometrial cytology, especially if combined with other markers. High p53 expression correlates with morphologic features of aggressiveness and the expression pattern of p53 correspond to the expected cyclic/atrophic pattern in normal endometrium.

High Expression of Ecto-Nucleotidases CD39 and CD73 in Human Endometrial Tumors

“…The usefulness of the touch prep technique for diagnosis has already been demonstrated in breast cancer with 100% sensitivity and specificity in the evaluation of tumor margins at the time of the surgery [ 29 ]. This technique has also been previously used to demonstrate by immunolabeling a decreased expression of P2X7 ATP receptor in endometrial cancer cells [ 30 ] and also, recently, the relationship between p53 expression and the tumor grade [ 31 ]. However, to our knowledge, this is the first report validating the use of touch preps for enzyme activity studies, therefore opening the possibility of performing such studies in cytological samples.…”

Comparative Study of Different Histologic Classifications in the Degree of Differentiation in Endometrial Adenocarcinoma

“…Ki-67 expression was greater in tumors considered high grade in the cellular differentiation classifications. Other studies have found that Ki-67 expression was associated with tumor staging and histologic grading (24,25). The proliferation index, accessed by Ki-67 expression, has been considered a prognostic factor.…”

Evaluation of positive ductal margins of biliary tract cancer in intraoperative histological examination

“…The bile duct stump was also examined using immunohistochemical staining targeting Ki67 and p53. Ki67 is a prognostic marker of tumour proliferation that has been extensively researched in retrospective studies (22). Its cellular location is strongly cell cycle dependent (23,24).…”

“…Its cellular location is strongly cell cycle dependent (23,24). Ki67 levels are low during G1 and early S phase and peak during mitosis (22). p53 functions as a transcription factor involved in cell-cycle control, DNA repair, apoptosis and cellular stress responses (25).…”