Expression of T-Cell Markers on Chicken Bone Marrow Precursor Cells Incubated with an Avian Thymic Hormone

Avian thymic hormone (ATH), a parvalbumin with immunomodulating properties, was used as antigen for development of a hybridoma cell line. Hybridoma screening by enzyme-linked immunosorbent assay (ELISA) led to the culture, purification, and characterization of an hybridoma, designated 4A6, which secretes IgG1 that is specific for ATH. The monoclonal antibody (MAb) was characterized by techniques involving indirect competitive and non-competitive ELISA, Western Blot analysis, and immunohistological staining. The MAb was highly specific for ATH with no cross-reactivity to other chicken tissue extracts or commercially available parvalbumins.

show abstract

“…In vitro studies have demonstrated the ability of ATH to induce expression of T-cell markers on chicken bone marrow cells in culture (4).…”

Section: Introductionmentioning

confidence: 99%

Monoclonal Antibody for Avian Thymic Hormone

Hall

Beach²,

Ragland³

1991

Hybridoma

View full text Add to dashboard Cite

show abstract

“…A number of questions remain unanswered relative to these mediators, however. First, most of these have been detected using molecular biology techniques, and, although transcribed mRNA may be shown to be present, it Siegel et al, 1985;Smith et al, 1986;Zurawski et al, 1986Kurland and Bockman, 1978Smith et al, 1983Kruger et al, 1989Weigent et al, 1988Aquila et al, 1991Heistand et al, 1986Hartmann et al, 1989Greenen et al, 1986Greenen et al, 1986Harbour-McMenamin et al, 1986Cutz et al, 1978O'Dorisio et al, 1980Bach et al, 1977Coudert et al, 1979;Marsh et al, 1991Murthy et al, 1984Goldstein and Audhya, 1987Goldstein et al, 1981Goldstein and Audhya, 1987 remains unclear which of these products are translated and secreted in physiologically relevant quantities. Second, even for the gene products that have been shown to be released, it is not clear what biological role they may actually play.…”

Section: Production Of "Classical" Endocrine Products By the Immune Smentioning

confidence: 97%

“…These are avian thymic hormone or ATH (Murthy et al, 1984) and thymulin (Coudert et al, 1979;Marsh et al, 1991). Of these, the gene coding for ATH has been cloned and the 108 amino acid polypeptide characterized (Brewer et al, 1990).…”

Section: Cytokines and Hormonal Products Of The Lymphoid Tissuesmentioning

confidence: 97%

Neuroendocrine-Immune Interactions

Marsh

Scanes

1994

Poultry Science

View full text Add to dashboard Cite

The role of the neuroendocrine system in influencing both immune development and function has become an area of active research within many model systems, including the chicken. It is now clear that the neuroendocrine system can exert immediate feedback regulation on the immune system as well as control specific aspects of immune differentiation and development. The primary lymphoid organs of avian species (i.e., the thymus and the bursa of Fabricius) are also known to function as endocrine organs. These produce hormonal products that influence the development of lymphoid cells and that may feed back on the neuroendocrine system. In conjunction with the endocrine activities of the primary lymphoid organs, immune and accessory cells are known to produce a variety of secreted products or cytokines that have the potential not only for the regulation of immune function but also for mediating neuroendocrine activities. Finally, it has been demonstrated in a variety of species that leukocytes are capable of producing endocrine mediators previously believed to be produced only under the direct control of the hypothalamic-pituitary axis. Thus, there are numerous possibilities for bidirectional interactions between the immune and neuroendocrine systems. This discussion focuses primarily on these interactions with an emphasis on the means by which the hormonal mediators, growth hormone and thyroid hormone, may affect the thymus and the thymic microenvironment. The role of the adrenocorticoids and gonadal steroids in regulating immune function and their involvement in immune feedback circuits are also discussed.

show abstract

“…It is present in the blood stream where its concentration oscillates with a period of five days [17]. The protein also stimulates bone-marrow precursor cells in culture to express T cell markers [18]. Additionally, it stimulates blastogenic responses of one-month-old chickens injected either during the first week of life or between days 8 through 28 [19].…”

Section: Introductionmentioning

confidence: 99%