Expression of Th1, Th2 and immunosuppressive cytokine gene transcripts in canine atopic dermatitis

Nuttall, Tim; Knight, Pamela; McALEESE, Sybil M.; Lamb, Jonathan R.; Hill, Peter

doi:10.1046/j.1365-2222.2002.01356.x

Cited by 111 publications

(133 citation statements)

References 49 publications

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“…Moreover, the TGF-β expression was increased by Hsp60 in healthy dog skin, at 48 and 72 h after stimulation but only significant at the latter. A previous study found a higher constitutive expression of TGF-β in healthy canine skin compared to atopic skin, which was thought to be associated with clinical tolerance (Nuttall et al 2002). However, no difference in TGF-β expression was found in the unstimulated skin between atopic and control skin in the present study.…”

Section: Discussioncontrasting

confidence: 74%

Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Lee

Rutten

Kooten

et al. 2008

Cell Stress and Chaperones

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The purpose of this study was to investigate the immunoregulatory potential of Hsp60 in the skin of dogs with atopic dermatitis. Three dogs with chronic atopic dermatitis and four healthy dogs were injected intradermally with Hsp60 and phosphate-buffered saline. Biopsies were taken before testing from non-injected control skin, lesional and non-lesional atopic skin, and 48 and 72 h after injection. Analysis of cytokine messenger RNA was performed using quantitative real-time polymerase chain reaction. Forty-eight hours after Hsp60 injection, a rise in interleukin (IL)-10 was found (P=0.034) with the highest expression levels in non-lesional atopic and control skin. A rise of transforming growth factor beta (P=0.015) and IL12p40 (P=0.017) was noticed 72 h after Hsp60 injection in control skin. No significant differences were observed for the expression of IL-4, IL-12p35, and interferon gamma. The results indicate that Hsp60 is able to induce cytokines of a regulatory and Th1 phenotype in the skin. Furthermore, this study seems to provide a first indication of deficient Hsp60 response in atopic dermatitis affected skin.

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Section: Discussioncontrasting

confidence: 74%

Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Lee

Rutten

Kooten

et al. 2008

Cell Stress and Chaperones

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“…NLS skin had increased expression of IL- 4 and Foxp3, which may indicate that NLS skin is primed to become LS skin. In comparison, the cutaneous inflammation in dogs with spontaneous AD was characterised by expression of IL-6, TARC, IL-4 and IL-13 mRNA in the early stages, and IFN-c, IL-12 and IL-18 in the chronic stages and no data are available on Foxp3 expression levels (Olivry et al, 1999;Nuttall et al, 2002;Marsella et al, 2006 (Cosmi et al, 2003;Jarvis et al, 2005;James and Kwok, 2007;Joosten et al, 2007;Mahic et al, 2008;Billerbeck et al, 2009). It would be interesting to investigate whether the CD8 + T cells are responsible for…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, cell subsets and cytokine production in the skin of dogs with AD have been investigated extensively. In AD, inflammation is characterised by an influx of CD4 + and CD8 + T cells in the lesional skin (Olivry et al, 1997;Sinke et al, 1997) and a mixed cytokine profile with predominant expression of IL-6, TARC, IL-4 and IL-13 genes in the early stage followed by IFN-c, IL-12 and IL-18 later on (Olivry et al, 1999;Nuttall et al, 2002;Marsella et al, 2006). Whereas most dogs with AD have circulating allergen-specific IgE (DeBoer and Hillier, 2001), there is controversial evidence for a similar reaction phenomenon in dogs with spontaneous manifestations of CAFRs (Jackson et al, 2003;Pucheu-Haston et al, 2008;Puigdemont et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

Veenhof

Knol

Schlotter

et al. 2011

The Veterinary Journal

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a b s t r a c tThe immunopathogenesis of cutaneous adverse food reactions (CAFRs) in dogs is unknown. Since the clinical manifestations in the skin are like those found in canine atopic dermatitis (AD), this study investigated the similarity in T cell phenotypes and gene expression of cytokines and transcription factors in CAFRs. In addition, the influence of an elimination diet on these parameters was tested.In the skin of canine CAFRs, a predominant presence of CD8 + T cells and increased expression of the IL-4, IL-13, Foxp3 and SOCS-3 genes were observed. IFN-c gene expression was increased in lesional compared to non-lesional skin. The predominance of CD8 + T cells indicates that the immunopathogenesis of CAFRs is different from that of canine AD. The elimination diet relieved clinical signs, but did not influence T cell phenotypes or expression of the cytokine and transcription factor genes in the skin of dogs with CAFRs, indicating a continuously pre-activated immune status in dogs sensitised to food constituents.

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“…Histopathological analysis revealed that CD4 + cells were markedly infil-trated in lesional skin of canine AD [26]. A recent study indicated that IL-4 mRNA was preferentially expressed in lesional skin of dogs with AD [20]. In lesional skin of canine AD, expression of CCL17 [12] and CCR4 mRNA [14] was detected in conjunction with the expression of inflammatory cytokines including IL-1β, IFN-γ and TNF-α [12].…”

mentioning

confidence: 95%

Expression Analysis of CCL27 and CCL28 mRNA in Lesional and Non-Lesional Skin of Dogs with Atopic Dermatitis

Maeda

Tsuchida

Shibata

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Chemokines are important regulators of the selective recruitment of inflammatory cells into sites of allergic inflammation. Since canine atopic dermatitis (AD) shares many clinical features of human AD, patterns of chemokine production in dogs may also be similar with those in humans. The aim of this study was to examine mRNA expression of CCL27 and CCL28 in lesional skin of dogs with AD to demonstrate similarity of chemokine production with human counterparts. RNA was extracted from skin biopsy specimens of 12 dogs with AD. The mRNA expression of CC chemokines (CCL4, CCL19, CCL20, CCL21, CCL24, CCL27 and CCL28) was analyzed by quantitative real-time PCR and was compared between lesional and non-lesional skin. Seven types of chemokines examined were constitutively expressed in both lesional and non-lesional skin. It was found that mRNA expression levels of CCL27 and CCL28 among the chemokines were significantly different between lesional and non-lesional skin (P<0.05). Expression level of CCL27 mRNA in lesional skin was significantly lower than that in non-lesional skin. On the other hand, CCL28 mRNA expression in lesional skin was found to be higher than that in non-lesional skin. These results suggest that CCL28 but not CCL27 may play important roles in immunopathogenesis of canine AD, indicating that experimental canine study may provide additional information that can be extrapolated to human AD.

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Expression of Th1, Th2 and immunosuppressive cytokine gene transcripts in canine atopic dermatitis

Cited by 111 publications

References 49 publications

Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

Expression Analysis of CCL27 and CCL28 mRNA in Lesional and Non-Lesional Skin of Dogs with Atopic Dermatitis

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