Expression of the MuIFNα7 gene in Bacillus subtilis using the levansucrase system

Protein secretion in Bacillus species.

“…One approach to avoid degradation is to produce the foreign proteins in the exponential growth phase, when relatively small amounts of proteases are secreted (29,58,62,403). However, in batch cultures the exponential phase is too short and the cell density is too low for production purposes.…”

Transcripts of the genes sacB, amyE, sacC and csn expressed in Bacillus subtilis under the control of the 5′ untranslated sacR region display different stabilities that can be modulated

“…Extracellular levansucrase (SacB), expressed under the control of its own leader region (sacR), is overproduced in a degU32(Hy) mutant during the exponential phase of growth (Chambert & Petit-Glatron, 1984) under conditions of full induction by sucrose. This sacR leader region has therefore been widely used for the inducible secretion of heterologous proteins during the exponential phase of growth (Joliff et al, 1989 ;Dion et al, 1989 ;Wong, 1989 ;Petit et al, 1990). Surprisingly, the extracellular production of these proteins, including those originating from other bacilli (Joyet et al, 1986), is within one order of magnitude lower than that of levansucrase.…”

Peptide carrier potentiality of Bacillus subtilis levansucrase

“…These properties make the expression/secretion signals of s a d particularly attractive for the construction of secretion vectors (Wong, 1989). However, attempts to translocate normally intracellular prokaryotic proteins or extracellular eukaryotic proteins by fusing their genes to the sacB signal sequence have so far failed (Dion et al, 1989), the secretion block occurring at the post-translational level (Sarvas, 1986).…”