Expression of the novel protein PTPIP51 in rat liver: an immunohistochemical study

Schreiner, Dietmar; Tag, Claudia; Wimmer, Monika

doi:10.1007/s00418-007-0298-2

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…PTPIP51 lacking this N-terminal sequence shows a cytosolic localization and is unable to induce programmed cell death. This is in accordance with previous observations (Stenzinger et al 2007). Pathre and colleagues (2001) reported neurite outgrowth in the chicken retina to be regulated by a molecular system including a cleaved form of PTP1B as an integrated component.…”

Section: Retinal Developmentsupporting

confidence: 94%

Expression of PTPIP51 during mouse eye development

Maerker

Schreiner

Tag

et al. 2007

Histochem Cell Biol

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The expression pattern of the novel tyrosine phosphatase interacting protein 51 (PTPIP51) was studied during the organogenesis of mouse eye on a transcriptional (RT-PCR and in situ hybridization) and translational level (immunohistochemistry and immunoblotting). Timed developmental stages from day E12 to day E18 were analyzed regarding the distribution of PTPIP51 and compared to the expression patterns observed during postnatal developmental stages and adult eye. PTPIP51 was found to be expressed in all investigated developmental stages in derivatives of mesoderm and ectoderm, such as developing cornea, lens, neuroretina and extraocular muscles. Conjuctiva and corneal epithelia were PTPIP51 reactive during all investigated developmental stages including the mature eye. Embryonic differentiation led to reactive keratocytes of the corneal stroma and remained so in post partal stages, as well as in the adult eye. On day E12, all cells comprising the developing lens body showed PTPIP51 expression. Further development unto the adult eye resulted in a restriction of PTPIP51 expression to the anterior lens epithelium and finally to the equatorial region of the lens epithelium. The developing neuroretina showed a strong PTPIP51 expression in the inner neuroblastic layer and the future receptor cell layer. In the adult eye, the retinal ganglion cells and the inner nuclear layer remained PTPIP51 reactive. The data presented here suggests PTPIP51 to be integrated in signaling cascades regulating differentiation and apoptosis during eye development.

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Section: Retinal Developmentsupporting

confidence: 94%

Expression of PTPIP51 during mouse eye development

Maerker

Schreiner

Tag

et al. 2007

Histochem Cell Biol

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“…These findings may be explained by putative isoforms or splicing variants expressed in different tissues that lack this N‐terminal sequence, which is mandatory for the execution of apoptosis [4, 8]. This hypothesis is supported by the fact that PTPIP51 was not found to be exclusively restricted to mitochondria but was present at the cellular membrane and to a lower extent in the cytosol of malignant keratinocytes [7, 8, 36]. The intracellular localization may also be explained by an in vivo interaction with PTP1B or Src‐kinases.…”

Section: Discussionmentioning

confidence: 99%

The novel protein PTPIP51 is expressed in human keratinocyte carcinomas and their surrounding stroma

Koch

Viard

Märker

et al. 2008

J Cellular Molecular Medi

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Background: The novel protein PTPIP51 (SwissProt accession code Q96SD6) is known to interact with two non-transmembrane protein-tyrosine phosphatases, PTP1B and TCPTP in vitro. Overexpression of the full-length protein induces apoptosis in HeLa and HEK293T cells (Lv et al. 2006). PTPIP51 shows a tissue-specific expression pattern and is associated with cellular differentiation and apoptosis in some mammalian tissues, especially in human follicular and interfollicular epidermis. PTPIP51 protein is expressed in all suprabasal layers of normal epidermis, whereas the basal layer contains PTPIP51 mRNA only but lacks the protein. Objectives: The expression of PTPIP51 was investigated in keratinocyte carcinomas, that is human basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) as well as Bowen's disease (BD) and keratoacanthomas (KAs) on a transcriptional (mRNA) and translational (immunohistochemical) level. Methods: Formalin-fixed, paraffin-embedded sections of BCCs, SCCs, KAs and BD, respectively, were analysed by RT-PCR, as well as immunohistochemistry and subsequent fluorescence microscopy. PTPIP51-positive cells of the tumour and the surrounding stroma were identified on the basis of specific morphological features by means of H & E staining. To obtain further information about a putative function of PTPIP51, a possible association of PTPIP51 with apoptotic cells, as well as an assumed negative correlation with proliferating cells was investigated by means of an in-situ TUNEL assay and Ki67/MIB-1 antigen staining, respectively. Co-immunostainings with PTPIP51 were performed for the following antigens: TCPTP, PTP1B and β-catenin. Results: PTPIP51-expression was detected in BCCs and SCCs of the skin, as well as in KAs and BD. Both types of keratinocyte carcinoma revealed a specific localization pattern of PTPIP51 in malignant keratinocytes. Whereas PTPIP51 -positive cells of BCC were found to form two cluster types with a different subcellular localization of the protein, i.e. cytoplasmic and nuclear or predominantly membranous, investigation of SCC revealed a meshwork-like appearance of PTPIP51-positive malignant keratinocytes, created by a mainly membranous localization. BD and KA resembled the findings of PTPIP51-expression in SCC. Furthermore, we observed a partial co-localization of PTP1B and PTPIP51 in BCC. SCC and BCC showed a co-expression and partial co-localization of PTPIP51 with β-catenin. Some PTPIP51-positive cells were found to undergo apoptosis. PTPIP51 was also expressed in cells comprising the surrounding stromal microenvironment. This was particularly noticed for endothelial cells lining peritumoural vessels as well as for infiltrating cells of both, the innate and the adaptive immune system. Conclusions: The results showed a distinct mainly membranous expression pattern of PTPIP51 in BCCs and SCCs. Since PTPIP51 was also detected in the peritumoural tissue, the protein may play a crucial role in keratinocyte tumour development.

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“…Recently, it was reported that expression of the the novel tyrosine phosphatase interacting protein 51 (PTPIP51), a protein which is present in diVerent mammalian species and tissues (e.g., Stenzinger et al 2007) and which has been suggested in in vitro studies to be involved in diVerentiation and programmed cell death, is present in ganglion cell layers and photoreceptor cells and is downregulated in cultured postnatal rat retinal explants by CNTF (Roger et al 2007). To elucidate a possible role of PTPIP51 in eye development, Maerker et al (2008) studied the expression of PTPIP51 during the organogenesis of the mouse eye using RT-PCR and in situ hybridization, immunohistochemistry and Western blotting.…”

Section: Developmental Studiesmentioning

confidence: 98%

State-of-the-art technologies, current opinions and developments, and novel findings: news from the field of histochemistry and cell biology

Asan

Drenckhahn

2008

Histochem Cell Biol

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Investigations of cell and tissue structure and function using innovative methods and approaches have again yielded numerous exciting findings in recent months and have added important data to current knowledge, inspiring new ideas and hypotheses in various fields of modern life sciences. Topics and contents of comprehensive expert reviews covering different aspects in methodological advances, cell biology, tissue function and morphology, and novel findings reported in original papers are summarized in the present review.

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Expression of the novel protein PTPIP51 in rat liver: an immunohistochemical study

Cited by 6 publications

References 45 publications

Expression of PTPIP51 during mouse eye development

Expression of PTPIP51 during mouse eye development

The novel protein PTPIP51 is expressed in human keratinocyte carcinomas and their surrounding stroma

State-of-the-art technologies, current opinions and developments, and novel findings: news from the field of histochemistry and cell biology

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