Expression of the transferrin gene during development of non-hepatic tissues: High level of transferrin mRNA in fetal muscle and adult brain

Levìn, Mariano J.; Tuil, David; Uzan, G; Dreyfus, Jean‐Claude; Kahn, Axel

doi:10.1016/0006-291x(84)90461-3

Cited by 127 publications

(47 citation statements)

References 11 publications

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“…3'-MeDAB-induced hepatoma is known to be a well differentiated slow-growing hepatoma, which synthesizes only minimal amounts of a-foetoprotein and express practically normal amounts of serum albumin [24]. These data were fully coiifirmed in our study and were extended to transferrin, which is mainly synthesized in foetal and adult liver and also, in lower amounts, in several foetal tissues [29].…”

Section: Discussionsupporting

confidence: 82%

“…R N A quantification was performed by dot-blot hybridization and subsequent counting of the individual dots by liquid scintillation [27,29,361. Results are expressed as radioactivity of specific probe hybridized/mass total RNA, and as a percentage of control values.…”

Section: Methodsmentioning

confidence: 99%

“…Our goal in this study was to establish an accurate correlation between the different stages of 3'-MeDAB hepatocarcinogenesis and the modifications of the expression of different genes encoding proteins specific for adult liver (aldolase B, L-type pyruvate kinase, serum albumin) for both adult and foetal liver (transferrin [29]); and for foetal tissues in general (aldolase A and an unidentified 2.7-kb mRNA species). In addition, inducibility of aldolase B and L-type pyruvate kinase by carbohydrates was investigated [26, 271. …”

mentioning

confidence: 99%

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Modifications of the expression of liver‐specific and non‐specific messenger RNAs during azo‐dye hepatocarcinogenesis

Lamas

Schweighoffer

van

et al. 1985

European Journal of Biochemistry

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The expression of specific and non-specific rat liver messenger RNAs has been studied during 3'-methyl-4-(dimethy1amino)azobenzene (3'-MeDAB) carcinogenesis, using cDNA probes complementary to inRNAs encoding aldolase A and B, L-type pyruvate kinase, albumin, a-foetoprotein, transferrin and an unidentified 2.7 x 103-base mRNA. mRNAs specific for undifferentiated cells, such as those encoding aldolase A and the unidentified 2.7 x 103-base species were re-expressed very early, being easily detectable at the 1st week of 3'-MeDAB treatment. They reached a maximum of expression at the 4th week. Simultaneously the levels of aldolase B and L-type pyruvate kinase mRNAs dramatically decreased as compared to controls, but remained responsive to induction by a high-carbohydrate diet.Albumin and transferrin mRNA levels were only slightly modified in the course of the carcinogenic diet. At the terminal stage of hepatocarcinogenesis, i. e. in malignant hepatoma cells, expression and inducibility of aldolase B and L-type pyruvate kinase mRNAs were similar to those in normal adult rats while mRNAs specific for undifferentiated or foetal stages were also synthesized.The very early changes in gene expression for aldolases A and B, L-type pyruvate kinase and the 2.7 x lo3-base mRNA species could indicate that carcinogenic diet modifies gene control mechanisms long before inducing hepatoma.I t is well established that rats fed a 3'-methyl-4-(dimethy1amino)azobenzene (3'-MeDAB)-containing diet for twenty weeks develop a well-differentiated (slow-growing) hepatoma [I]. Carcinogenesis is associated with very early histological and biochemical alterations : necrosis of hepatocytes with appearance of new cellular populations and, simultaneously, resurgence of foetal proteins and a decrease in the expression of adult specific markers as shown first by Schapira et al. for aldolase [2] and then later extended to other enzymes and proteins [3 -171. Aldolase B [7, 81 and L-type pyruvate kinase [9, I51 are characteristic of normal adult liver and are decreased in hepatoma cells, while the foetal-type isozymes, aldolases A and C, and M2-type pyruvate kinase increase [7,8,[10][11][12] 151. a-Foetoprotein, a foetal equivalent of serum albumin, is also re-expressed during hepatocarcinogenesis [ 5 , 6, 13, 141.The expression of the albumin and a-foetoprotein genes during liver development, regeneration and carcinogenesis has been widely investigated. a-Foetoprotein gene expression switches off at birth and conversely albumin gene expression switches on; phenotypic expression of a-foetoprotein is resumed in the adult rat during regeneration, exposure to chemical hepatocarcinogenes and in hepatoma [I 8 -241.During 3'-MeDAB hepatocarcinogenesis there is re-expression of a-foetoprotein but albumin mRNA is unchanged [24] decreased while aldolase A mRNA increased in carcinogentreated liver [25].However, the mechanisms involved in regulation of isozynie expression are not yet as clearly understood as are those leading to the resurgence of a-foetoprotein....

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Section: Discussionsupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Modifications of the expression of liver‐specific and non‐specific messenger RNAs during azo‐dye hepatocarcinogenesis

Lamas

Schweighoffer

van

et al. 1985

European Journal of Biochemistry

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“…Transferrin mRNA was also detected in rat uterus. Transferrin mRNA is found in low levels in muscle tissue [8,11], however…”

Section: Discussionmentioning

confidence: 99%

The expression of genes coding for positive acute‐phase proteins in the reproductive tract of the female rat

Thomas

Schreiber

1989

FEBS Letters

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High levels of ceruloplasmin mRNA were measured in the uterus of both pregnant and non‐pregnant rats. No mRNA for α2‐macroglobulin and α1‐acid glycoprotein could be detected in the uterus in contrast to the high levels of those two mRNAs found in the decidua in the mid‐gestation period. Synthesis of plasma proteins with a protective function in the decidua or uterus may be important in maintaining homeostasis at different stages of reproduction. In addition, ceruloplasmin synthesis by the uterus may be part of a system transporting copper to the fetus.

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“…Denatured RNAs were transferred to Zetabind membranes (AMF, Meriden, Conn.) by electroblotting. Probes for rat albumin (30), rat transferrin (19), rat TAT (31), rat AGP (29), rat 13-actin (B. Patterson, National Institutes of Health, Bethesda, Md. ), and rat glucocorticoid receptor (21) were nick translated immediately prior to hybridization.…”

Section: Methodsmentioning

confidence: 99%

Regulation of rat liver maturation in vitro by glucocorticoids.

Chou¹,

Wan²,

Sakiyama³

1988

Mol. Cell. Biol.

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The biochemistry of liver maturation was studied by using the RLA209-15 fetal rat hepatocyte line that is temperature sensitive for maintenance of the differentiated fetal liver phenotype. At 33°C these cells were dedifferentiated; but at 40°C they were phenotypically differentiated and, like normal fetal hepatocytes, synthesized moderate levels of albumin and transferrin, high levels of authentic (69,000 and 73,000 molecular weight) rat fetal a-fetoprotein (AFP), and low levels of a 65,000-molecular-weight variant AFP. Our results indicated that administration of glucocorticoid hormones to RLA209-15 cells at 40°C induced a series of events associated with normal hepatocyte maturation; synthesis of fetal AFP was inhibited, whereas the synthesis of variant AFP, albumin, transferrin, tyrosine aminotransferase, and al-acid glycoprotein was induced.,The variant AFP was produced by RLA209-15 cells at both temperatures and was encoded by an mRNA of 1.7 kilobases (kb). The fetal AFP was encoded by an mRNA of 2.2 kb. Normal adult rat liver contained three AFP mRNAs of 2.2 (minor), 1.7, and 1.5 kb. The 1.7-kb adult liver AFP mRNA comigrated with the RNA found in RLA209-15 cells, and both directed the synthesis of a 50,000-molecular-weight precursor polypeptide of the variant AFP. Administration of glucocorticoids to RLA209-15 cells grown at 33°C stimulated synthesis of both the fetal and variant AFPs, but the levels of the 2.2-kb AFP mRNA were preferentially increased. RLA209-15 cells contained two glucocorticoid receptor mRNAs of 6.8 and 4.5 kb. The glucocorticoid-mediated maturation described above was blocked by the antiglucocorticoid RU486.

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Expression of the transferrin gene during development of non-hepatic tissues: High level of transferrin mRNA in fetal muscle and adult brain

Cited by 127 publications

References 11 publications

Modifications of the expression of liver‐specific and non‐specific messenger RNAs during azo‐dye hepatocarcinogenesis

Modifications of the expression of liver‐specific and non‐specific messenger RNAs during azo‐dye hepatocarcinogenesis

The expression of genes coding for positive acute‐phase proteins in the reproductive tract of the female rat

Regulation of rat liver maturation in vitro by glucocorticoids.

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