Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes

Friedrich, M.; Grahnert, Andreas; Paasch, Uwe; Tannapfel, Andrea; Koch-Nolte, Friedrich; Hauschildt, Sunna

doi:10.1111/j.1745-7262.2006.00125.x

Cited by 15 publications

(15 citation statements)

References 18 publications

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“…However, through bioinformatics analysis, we found that the bovine ART3 protein has no enzymatic active site motif, and consequently, cannot transfer ADP-ribose and cause ADP ribosylation of the target protein. In addition, HEK-293-T cell transfection (45) and DC27.10 cell overexpression (43) experiments showed that ART3 has no ADP-ribosyltransferase activity, which confirmed our bioinformatics analysis. ART3 has also been detected in human spermatocytes and is significantly related to human non-obstructive azoospermia (46).…”

Section: Discussionsupporting

confidence: 79%

Insights into the mechanism of bovine spermiogenesis based on comparative transcriptomic studies

Duan

et al. 2020

Preprint

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To reduce the reproductive loss caused by semen quality and provide theoretical guidance for the eradication of human male infertility, differential analysis of the bovine transcriptome among round spermatids, elongated spermatids, and epididymal sperm was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, the Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction network (PPI network), and bioinformatics analysis uncovered the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, glycosylation, and the functional gene ART3 may play an important role during spermiogenesis. Therefore, its localisation in the seminiferous tubule was investigated by immunofluorescent analysis, and its structure and function were also predicted. This study provides important data for revealing the mystery of life during spermiogenesis resulting from acrosome formation, histone replacement, and the fine regulation of gene expression.

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Section: Discussionsupporting

confidence: 79%

Insights into the mechanism of bovine spermiogenesis based on comparative transcriptomic studies

Duan

et al. 2020

Preprint

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“…In addition, the importance of CKLFSF2 in spermatogenesis was suggested by a close correlation between CKLFSF2 abnormal expression and a spermatogenesis defect (Figures 4, 5). With the aggravation of the spermatogenesis defect, the CKLFSF2-positive cell numbers and mRNA level decreased significantly with no expres-sion in the testes of patients with SCOS, which is characterized histologically by a complete loss of the germinal epithelium in testicular tubules [11][12][13]. This correlation, together with the cell-and stage-specific expression, provided compelling evidence for a crucial role of CKLFSF2 in spermatogenesis.…”

Section: Discussionmentioning

confidence: 93%

“…It demonstrated that CKLFSF2 was co-localized with the ER marker and near the Golgi apparatus, but not co-localized with Cox7a2 ( Figure 6). (1)(2)(3)(4)(5)(6), spermatogenesis arrest (7)(8)(9) or spermatogenesis disturbance (10)(11)(12)(13)(14). CKLFSF2 was not detected in all patients with Sertoli cell-only syndrome or spermatogenesis arrest.…”

mentioning

confidence: 99%

Expression and localization of CKLFSF2 in human spermatogenesis

et al. 2007

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“…The sequence arginine-serine-glutamate-glutamineglutamate (R-S-EQE; which spans regions 1-2-3, respectively) is typical of the arginine-specific ARTs, and it is found in all of the active ARTC enzymes: ARTC1, 2 and 5. ARTC3 and ARTC4 do not have measurable activities (89,90), and thus their roles in cell biology are independent of ART activity, a situation that is also seen in the ARTD family, which includes the inactive ARTD9 and ARTD13 members, and in the sirtuin family, which is composed of seven members, only two of which are active as mono-ARTs.…”

Section: The Mono-artcsmentioning

confidence: 99%

State of the art of protein mono-ADP-ribosylation biological role and therapeutic potential

Fabrizio

2015

Front Biosci

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Mono-ADP-ribosylation is a post-translational modification that was discovered more than five decades ago, and it consists of the enzymatic transfer of ADP-ribose from NAD⁺ to acceptor proteins. In viruses and prokaryotes, mono-ADP-ribosylation is mainly, but not exclusively, a mechanism used to take control of the host cell. In mammals, mono-ADP-ribosylation serves to regulate protein functions, and it is catalysed by two families of toxin-related cellular ADP-ribosyltransferases: ecto-enzymes that modify various cell-surface proteins, like integrins and receptors, and intracellular enzymes that act on a variety of nuclear and cytosolic proteins. These two families have been recently renamed the ARTCs (clostridia toxin like) and ARTDs (diphtheria toxin like), depending on their conserved structural features, and in terms of their relationships to the bacterial toxins. In addition, two members of the structurally non-related sirtuin family can also modify cellular proteins by mono-ADP-ribosylation. Recently, new examples of ADP-ribosylation of proteins involved in signal transduction and intracellular trafficking have been discovered, thus opening the route to the better molecular understanding of this reaction and of its role in human cell physiology and pathology.

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Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes

Abstract: Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.

Cited by 15 publications

References 18 publications

Insights into the mechanism of bovine spermiogenesis based on comparative transcriptomic studies

Insights into the mechanism of bovine spermiogenesis based on comparative transcriptomic studies

Expression and localization of CKLFSF2 in human spermatogenesis

State of the art of protein mono-ADP-ribosylation biological role and therapeutic potential

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