Expression of VEGF-system mRNAs in mouse blastocysts is not affected by in vitro culture

Ingmann, S.; Hirchenhain, Jens; Huse, Isabelle; Hess, Alexandra P.; Bielfeld, P.; Kruessel, J.S.

doi:10.1016/s0015-0282(02)03946-8

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VEGFA binds and signals via two tyrosine kinase receptors, VEGFR1 and VEGFR2, both of which are expressed by mouse blastocysts [106]. Functional studies demonstrate the ability of VEGF, secreted by endometrial epithelial cells in culture and into the uterine cavity, to significantly enhance mouse blastocyst outgrowth on fibronectin [76].…”

Section: Effects Of Endometrial-derived Immune Regulators On the Preimentioning

confidence: 99%

Alternate roles for immune regulators: establishing endometrial receptivity for implantation

Hannan

Evans

Salamonsen

2011

Expert Review of Clinical Immunology

View full text Add to dashboard Cite

Many immune regulators are now identified as having key roles at the embryo-maternal interface. Importantly, a cohort of cytokines, chemokines and growth factors are produced by the endometrial glands and secreted into the uterine cavity where they act both on the blastocyst and on the endometrial surface, changing adhesive capacity, modifying blastocyst development and outgrowth and providing chemoattraction, in addition to their previously known functions in immune regulation. As implantation progresses to highly controlled invasion of the trophoblast through the maternal decidua, similar factors produced by glands, decidual cells and cells of the innate immune system are critical for guiding the trophoblast to the maternal vasculature and establishing a functional placenta. Disturbance to production or action of such mediators can result in loss of uterine receptivity (manifesting as infertility) or disorders of early pregnancy including recurrent miscarriage and preeclampsia.

show abstract

Section: Effects Of Endometrial-derived Immune Regulators On the Preimentioning

confidence: 99%

Alternate roles for immune regulators: establishing endometrial receptivity for implantation

Hannan

Evans

Salamonsen

2011

Expert Review of Clinical Immunology

View full text Add to dashboard Cite

show abstract

Effects of vascular endothelial growth factor on porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer

et al. 2011

View full text Add to dashboard Cite

Oxygen-Regulated Gene Expression in Bovine Blastocysts1

Harvey

Kind

Pantaleon

et al. 2004

Biology of Reproduction

155

View full text Add to dashboard Cite

Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFalpha protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1alpha, HIF2alpha) was unaltered by oxygen concentration. HIF2alpha protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1alpha protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2alpha.

show abstract

Expression of VEGF-system mRNAs in mouse blastocysts is not affected by in vitro culture

Cited by 3 publications

References 0 publications

Alternate roles for immune regulators: establishing endometrial receptivity for implantation

Alternate roles for immune regulators: establishing endometrial receptivity for implantation

Effects of vascular endothelial growth factor on porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer

Oxygen-Regulated Gene Expression in Bovine Blastocysts1

Contact Info

Product

Resources

About