Expression profiling of homeobox genes in silk gland development in the mulberry silkworm Bombyx mori

Dhawan, Sangeeta; Gopinathan, K. P.

doi:10.1007/s00427-003-0357-1

Cited by 16 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TAAT motif, the essential sequence for binding of homeodomain proteins (Kissinger et al, 1990), was found several times in an up to À234 region. Actually, homeobox genes, Ultrabithorax, even-skipped (eve), and a Pax-like gene, were expressed in PSGs during intermolt stages (Dhawan and Gopinathan, 2003).…”

Section: Introductionmentioning

confidence: 99%

Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: Identification of a novel transcription enhancing element with a homeodomain protein-binding motif

Shimizu¹,

Ogawa²,

Hino³

et al. 2007

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: Identification of a novel transcription enhancing element with a homeodomain protein-binding motif

Shimizu¹,

Ogawa²,

Hino³

et al. 2007

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…Hox transcription factor gene that shows MSG-specific expression in the silk gland (23,31). The MSG is separated into three territories termed MSG-A, MSG-M, and MSG-P (Fig.…”

Section: Expression Of Sericin Genes Following Antp Misexpressionantpmentioning

confidence: 99%

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Tsubota

Tomita

Uchino

et al. 2016

Journal of Biological Chemistry

View full text Add to dashboard Cite

Hox genes play a pivotal role in the determination of anteroposterior axis specificity during bilaterian animal development. They do so by acting as a master control and regulating the expression of genes important for development. Recently, however, we showed that Hox genes can also function in terminally differentiated tissue of the lepidopteran Bombyx mori. In this species, Antennapedia (Antp) regulates expression of sericin-1, a major silk protein gene, in the silk gland. Here, we investigated whether Antp can regulate expression of multiple genes in this tissue. By means of proteomic, RT-PCR, and in situ hybridization analyses, we demonstrate that misexpression of Antp in the posterior silk gland induced ectopic expression of major silk protein genes such as sericin-3, fhxh4, and fhxh5. These genes are normally expressed specifically in the middle silk gland as is Antp. Therefore, the evidence strongly suggests that Antp activates these silk protein genes in the middle silk gland. The putative sericin-1 activator complex (middle silk gland-intermoltspecific complex) can bind to the upstream regions of these genes, suggesting that Antp directly activates their expression. We also found that the pattern of gene expression was well conserved between B. mori and the wild species Bombyx mandarina, indicating that the gene regulation mechanism identified here is an evolutionarily conserved mechanism and not an artifact of the domestication of B. mori. We suggest that Hox genes have a role as a master control in terminally differentiated tissues, possibly acting as a primary regulator for a range of physiological processes.

show abstract

“…The cells in SG pursue an endoreplicative cycle, it is believed that there are should be obvious expression of G1/S complex and no expression of mitotic molecules (Dhawan and Gopinathan, 2003b). However, it is reported that cyclin B expression in silkgland can be assayed in transcription and protein level (Sudhakar and Gopinathan, 2000;Zhang et al, 2006).…”

Section: Signal Transduction Pathwaymentioning

confidence: 98%

“…For example, the different isoforms expression of Broad-Complex may be related to suborganspecific distribution in silk gland (Nishita and Takiya, 2004), and some Hox gene expressions in the silkgland are also turned off during the moults (Dhawan and Gopinathan, 2003b).…”

Section: Signal Transduction Pathwaymentioning

confidence: 99%

“…It is reported that throughout the fourth molt/fifth intermolt period, some Hox genes, such as Forkhead/silk gland factor 1 and POU-M1/silk gland factor 3, may be involved in specifying compartments in the silkgland (Dhawan and Gopinathan, 2003b;Kokubo et al, 1996). Considering the dual function of Forkhead that is involved at early stages in the differentiation of the secretary cells in the embryo and later in the activation of the sericin genes, the spatial specific expression pattern and existence of multiple homeobox elements in silk fiber genes, suggests Hox genes, such as Pax-like gene, may also contribute to silk gene tissue-specific regulations in larva stage.…”

Section: Signal Transduction Pathwaymentioning

confidence: 99%

See 1 more Smart Citation

The gene expression profile of Bombyx mori silkgland

Tang

Zhang

et al. 2007

Gene

View full text Add to dashboard Cite

Expression profiling of homeobox genes in silk gland development in the mulberry silkworm Bombyx mori

Cited by 16 publications

References 30 publications

Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: Identification of a novel transcription enhancing element with a homeodomain protein-binding motif

Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: Identification of a novel transcription enhancing element with a homeodomain protein-binding motif

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

The gene expression profile of Bombyx mori silkgland

Contact Info

Product

Resources

About