Expression, Secretion, and Glycosylation of the 45- and 47-kDa Glycoprotein of
            <i>Mycobacterium tuberculosis</i>
            in
            <i>Streptomyces lividans</i>

Lara, María del Carmen Carmona; Servı́n‐González, Luis; Singh, Mahavir; Moreno, Carlos; Cohen, Ingrid; Nimtz, Manfred; Espitia, Clara

doi:10.1128/aem.70.2.679-685.2004

Cited by 55 publications

(63 citation statements)

References 41 publications

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“…These authors used the wild type Streptomyces lividans 66 strain 1326 (Kieser et al, 2000) transformed with plasmid pIJ6021MT-45 which carries the M. tuberculosis apa gene under a thiostrepton-inducible promoter, conferring also resistance to kanamycin (Lara et al, 2004). Flask cultures were made in triplicates using the conventional, three baffled and coiled flask configurations of 250 ml (filled with 50 ml) Erlenmeyer flasks (Pyrex, Mexico).…”

Section: Background: Culture Conditions and Biomass Propertiesmentioning

confidence: 99%

The flow inside shaking flasks and its implication for mycelial cultures

Palacios-Morales

Aguayo-Vallejo

Trujillo‐Roldán

et al. 2016

Chemical Engineering Science

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Section: Background: Culture Conditions and Biomass Propertiesmentioning

confidence: 99%

The flow inside shaking flasks and its implication for mycelial cultures

Palacios-Morales

Aguayo-Vallejo

Trujillo‐Roldán

et al. 2016

Chemical Engineering Science

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“…The primary reasons attributed for this include a significantly relaxed restriction-modification system which enhances exogenous DNA uptake [9] and considerably attenuated endogenous protease activity leading to improved product recovery from S. lividans [7]. Notable examples of biologically active heterologous protein productions in S. lividans include proteins of eukaryotic origins like human T-cell receptor CD4 [10], tumor necrosis factor-α [11], human interleukin [12] and salmon calcitonin [13] as well as bacterial proteins like mycobacterial antigens with appropriate glycosylation patterns [14]. Leucotropin™ – a recombinant therapeutic agent used in treatment of Hodgkin's disease is commercially produced through S. lividans fermentation (Cangene, Winnipeg, Canada).…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans

et al. 2007

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Background: The genomes of Streptomyces coelicolor and Streptomyces lividans bear a considerable degree of synteny. While S. coelicolor is the model streptomycete for studying antibiotic synthesis and differentiation, S. lividans is almost exclusively considered as the preferred host, among actinomycetes, for cloning and expression of exogenous DNA. We used whole genome microarrays as a comparative genomics tool for identifying the subtle differences between these two chromosomes.

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“…These studies indicate that mannose is an essential component of Apa as an antigen and in the modulation of T-cell-dependent immune responses (287,288). Lara et al showed that tuberculosis patients carry antibodies that react mainly to the carbohydrate part of Apa (530). M. tuberculosis targets the pulmonary surfactant protein A (PSP-A) C-type lectin of the innate immune system mainly via its lipoglycans, but the Apa antigenic glycoprotein was also suggested to aid in attachment to this receptor (531).…”

Section: Protein Glycosylationmentioning

confidence: 99%

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Tytgat

Lebeer

2014

Microbiol Mol Biol Rev

129

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SUMMARY Humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. However, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. Glycoconjugates are an interesting class of molecules for detailed studies, as they form a strain-specific barcode on the surface of bacteria, mediating specific interactions with the host. Strikingly, most glycoconjugates are synthesized by similar biosynthesis mechanisms. Bacteria can produce their major glycoconjugates by using a sequential or an en bloc mechanism, with both mechanistic options coexisting in many species for different macromolecules. In this review, these common themes are conceptualized and illustrated for all major classes of known bacterial glycoconjugates, with a special focus on the rather recently emergent field of glycosylated proteins. We describe the biosynthesis and importance of glycoconjugates in both pathogenic and beneficial bacteria and in both Gram-positive and -negative organisms. The focus lies on microorganisms important for human physiology. In addition, the potential for a better knowledge of bacterial glycoconjugates in the emerging field of glycoengineering and other perspectives is discussed.

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Expression, Secretion, and Glycosylation of the 45- and 47-kDa Glycoprotein of Mycobacterium tuberculosis in Streptomyces lividans

Cited by 55 publications

References 41 publications

The flow inside shaking flasks and its implication for mycelial cultures

The flow inside shaking flasks and its implication for mycelial cultures

Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

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