Extracellular DNA in blood products and its potential effects on transfusion

Li, Yang; Yang, Dongmei; Yang, Qian; Fu, Chaowei; Huang, Yurong

doi:10.1042/bsr20192770

Cited by 9 publications

(8 citation statements)

References 113 publications

(167 reference statements)

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“…PCs also contain mtDNA, which has been linked to transfusion reactions. 49,[96][97][98] In fact, Boudreau et al showed that activated platelets release mitochondria, both in encapsulated microparticles and as free organelles. The levels of extracellular mitochondria found in transfused PCs are higher in those having caused transfusion reactions (FNHTR cutaneous and cardiovascular symptoms) in transfusion patients.…”

Section: Pc Preparation and Storage Lesions Promote Secretion Of Biolmentioning

confidence: 99%

Effects and Side Effects of Platelet Transfusion

et al. 2021

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Aside from their canonical role in hemostasis, it is increasingly recognized that platelets have inflammatory functions and can regulate both adaptive and innate immune responses. The main topic this review aims to cover is the proinflammatory effects and side effects of platelet transfusion. Platelets prepared for transfusion are subject to stress injury upon collection, preparation, and storage. With these types of stress, they undergo morphologic, metabolic, and functional modulations which are likely to induce platelet activation and the release of biological response modifiers (BRMs). As a consequence, platelet concentrates (PCs) accumulate BRMs during processing and storage, and these BRMs are ultimately transfused alongside platelets. It has been shown that BRMs present in PCs can induce immune responses and posttransfusion reactions in the transfusion recipient. Several recent reports within the transfusion literature have investigated the concept of platelets as immune cells. Nevertheless, current and future investigations will face the challenge of encompassing the immunological role of platelets in the scope of transfusion.

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Section: Pc Preparation and Storage Lesions Promote Secretion Of Biolmentioning

confidence: 99%

Effects and Side Effects of Platelet Transfusion

et al. 2021

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“…From this study, we used 2-3 drops of blood sample or about 300 μ l. Normally, the amount of DNA from whole blood per ml is about 260–1,474 ng/ml [ 9 ] or 1,700 leucocyte-eq/ml [ 10 ]. Therefore, the 300 μ l of blood sample which used in this experiment is equal to 78–441 ng of DNA or 510 leucocytes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Time Course Detection of Human Male DNA from Blood Stains on Various Objects on Surface in a Natural Environment and in a Laboratory Using Loop-Mediated Isothermal Amplification (LAMP)

Kumsiri

Kanchanaphum

2021

Scientifica

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In forensic study, the biological evidence can easily degrade, especially DNA. Degraded and environmentally challenged samples can produce numerous problems in forensic DNA analysis including loss of band product. Loop-mediated isothermal amplification or LAMP is one of the DNA analysis techniques used in forensic study. This study explores the limitations of the efficiency of the LAMP technique on abandoned DNA. For the DNA template, 8 male and 2 female blood-stained samples were taken from the surfaces, namely, brick, cloth, and tile from inside, and buried outside the laboratory. The LAMP reaction was used to amplify the SRY gene for detecting male DNA. All the blood-stained samples were stored for 1, 7, 15, 30, and 45 day (s). The LAMP product from the blood-stained samples on all the surfaces that were kept in a laboratory was detected using the gel electrophoresis technique from day 1 until day 45. However, the LAMP product on day 30 and 45 was smear and dim. The LAMP product from the blood-stained samples buried outside the laboratory was observed using the gel electrophoresis technique within day 30 (smear and dim). To increase the efficiency of detection, the qLAMP technique detected product on all the male samples from all the surfaces buried outside the laboratory for 45 days. The results indicate that this LAMP condition was possible detecting male DNA and the environmental factors are the main influence on the sensitivity of the LAMP technique. In addition, the qLAMP technique can increase the capacity and sensitivity of the detection.

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“…While thorough investigation is lacking, some reports have indicated that cfDNA may have potential use in (i) biobanking; analysis of cfDNA in cord blood plasma has been useful for sample identification [ 115 ], (ii) forensic casework; cfDNA has been genetically profiled in samples recovered from externally found body fluids, such as blood, sweat, and feces [ 116 , 117 , 118 , 119 ], and (iii) quality control assurance of blood donations [ 120 ] or transfusions [ 121 ].…”

Section: Sequence Analysis Of Cfdnamentioning

confidence: 99%

New Perspectives on the Importance of Cell-Free DNA Biology

et al. 2022

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Body fluids are constantly replenished with a population of genetically diverse cell-free DNA (cfDNA) fragments, representing a vast reservoir of information reflecting real-time changes in the host and metagenome. As many body fluids can be collected non-invasively in a one-off and serial fashion, this reservoir can be tapped to develop assays for the diagnosis, prognosis, and monitoring of wide-ranging pathologies, such as solid tumors, fetal genetic abnormalities, rejected organ transplants, infections, and potentially many others. The translation of cfDNA research into useful clinical tests is gaining momentum, with recent progress being driven by rapidly evolving preanalytical and analytical procedures, integrated bioinformatics, and machine learning algorithms. Yet, despite these spectacular advances, cfDNA remains a very challenging analyte due to its immense heterogeneity and fluctuation in vivo. It is increasingly recognized that high-fidelity reconstruction of the information stored in cfDNA, and in turn the development of tests that are fit for clinical roll-out, requires a much deeper understanding of both the physico-chemical features of cfDNA and the biological, physiological, lifestyle, and environmental factors that modulate it. This is a daunting task, but with significant upsides. In this review we showed how expanded knowledge on cfDNA biology and faithful reverse-engineering of cfDNA samples promises to (i) augment the sensitivity and specificity of existing cfDNA assays; (ii) expand the repertoire of disease-specific cfDNA markers, thereby leading to the development of increasingly powerful assays; (iii) reshape personal molecular medicine; and (iv) have an unprecedented impact on genetics research.

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Extracellular DNA in blood products and its potential effects on transfusion

Cited by 9 publications

References 113 publications

Effects and Side Effects of Platelet Transfusion

Effects and Side Effects of Platelet Transfusion

Comparison of Time Course Detection of Human Male DNA from Blood Stains on Various Objects on Surface in a Natural Environment and in a Laboratory Using Loop-Mediated Isothermal Amplification (LAMP)

New Perspectives on the Importance of Cell-Free DNA Biology

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