Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs

Silva, Elisangela de Paula; Marti, Luciana Cavalheiro; Andreghetto, Flávia Maziero; Sales, Romário Oliveira de; Hoberman, Martin; Dias, Barbara dos Santos; Diniz, Larissa Figueiredo Alves; Santos, Alessandro Marins dos; Moysés, Raquel Ajub; Curioni, Otávio Alberto; López, Rossana Verónica Mendoza; Nunes, Fábio Daumas; Tajara, Eloíza H.; Severino, Patrícia

doi:10.1038/s41598-021-97753-y

Cited by 14 publications

(10 citation statements)

References 69 publications

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“…In contrast to our study, oral squamous cell carcinoma tissue also showed an enrichment for miR-24-3p compared to peritumoral tissue ( 56 ). Furthermore, the enrichment of miR-24-3p could be found in EVs derived from nasopharyngeal carcinoma cell lines and patient sera ( 57 ), head and neck cancer cell lines ( 58 ) and classical Hodgkin’s lymphoma ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

et al. 2022

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Extracellular vesicles (EVs) are important mediators in the intercellular communication, influencing the function and phenotype of different cell types within the tumor micro-milieu and thus promote tumor progression. Since EVs safely transport packages of proteins, lipids and also nucleic acids such as miRNAs, EVs and their cargo can serve as diagnostic and prognostic markers. Therefore, the aim of this study was to investigate EV embedded miRNAs specific for melanoma, which could serve as potential biomarkers. In contrast to previous studies, we not only analysed miRNAs from EVs, but also included the miRNA profiles from the EV-secreting cells to identify candidates as suitable biomarkers. While the characterization of EVs derived from normal melanocytes and melanoma cells showed largely comparable properties with regard to size distribution and expression of protein markers, the NGS analyses yielded marked differences for several miRNAs. While miRNA load of EVs derived from normal human epidermal melanocytes (NHEMs) and melanoma cells were very similar, they were highly different from their secreting cells. By comprehensive analyses, six miRNAs were identified to be enriched in both melanoma cells and melanoma cell-derived EVs. Of those, the accumulation of miR-92b-3p, miR-182-5p and miR-183-5p in EVs could be validated in vitro. By functional network generation and pathway enrichment analysis we revealed an association with different tumor entities and signaling pathways contributing melanoma progression. Furthermore, we found that miR-92b-3p, miR-182-5p and miR-183-5p were also enriched in EVs derived from serum of melanoma patients. Our results support the hypothesis that miRNAs derived from EVs can serve as prognostic or diagnostic liquid biopsy markers in melanoma. We identified EV-derived miRNAs and showed that those miRNAs, which were enriched in melanoma cells and EVs, are also found elevated in serum-derived EVs of patients with metastatic melanoma, but not in healthy subjects.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

et al. 2022

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“…DC-EV function depends on their molecular composition, in particular the concentration of co-stimulatory molecules (CD86, CD80, CD40) and antigen-presenting molecules (MHC-II, MHC-I) [ 286 , 287 ]. It was previously described that TEV cargo (HSP, TLR, HLA g [ 29 ]; S100A8, S100A9, Annexin A1 [ 30 ]; PGE2 [ 288 ]; TGFβ1 [ 289 ]; or microRNAs [ 290 ]), suppress DC maturation, resulting in the loss of tumour cell recognition [ 29 , 30 ]. Moreover, once activated by TEV, DC release their own TEV conveying tumour-associated antigens [ 291 ], which translate in T-cell cross-activation, antigen presentation, and immune tolerance [ 292 , 293 ].…”

Section: Tev Released By Tme Cellsmentioning

confidence: 99%

Tumour Derived Extracellular Vesicles: Challenging Target to Blunt Tumour Immune Evasion

Lopatina

Sarcinella

Brizzi

2022

Cancers

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Control of the immune response is crucial for tumour onset and progression. Tumour cells handle the immune reaction by means of secreted factors and extracellular vesicles (EV). Tumour-derived extracellular vesicles (TEV) play key roles in immune reprogramming by delivering their cargo to different immune cells. Tumour-surrounding tissues also contribute to tumour immune editing and evasion, tumour progression, and drug resistance via locally released TEV. Moreover, the increase in circulating TEV has suggested their underpinning role in tumour dissemination. This review brings together data referring to TEV-driven immune regulation and antitumour immune suppression. Attention was also dedicated to TEV-mediated drug resistance.

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“…Increasing evidence has highlighted how exosomes represent a mechanism underpinning cancer cell-triggered immune escape by inducing phenotypic changes in different immune cell populations. Paula Silva et al evaluated the potential effects of EVs originating from HNSCC cells on immune system response [ 47 ]. In this study, monocyte-derived dendritic cells (mono-DCs) were treated with EVs derived from oropharyngeal squamous cell carcinoma (OPHSCC) and OSCC cell lines [ 47 ].…”

Section: The Function Of Exosomes In Hnsccmentioning

confidence: 99%

“…Paula Silva et al evaluated the potential effects of EVs originating from HNSCC cells on immune system response [ 47 ]. In this study, monocyte-derived dendritic cells (mono-DCs) were treated with EVs derived from oropharyngeal squamous cell carcinoma (OPHSCC) and OSCC cell lines [ 47 ]. Impairment of mono-DC maturation and migration was observed following EV internalization.…”

Section: The Function Of Exosomes In Hnsccmentioning

confidence: 99%

“…Impairment of mono-DC maturation and migration was observed following EV internalization. Gene expression profiling in mDCs treated with EVs further reveals disrupted immune responses possibly targeted by miRNAs (e.g., miR-17-5p and miR-21) within EVs [ 47 ]. To test whether tumor- or T cell-derived exosomes in HNSCC patients’ plasma are immunosuppressive and impact disease activity, Theodoraki’s group separated CD3(–) from CD3(+) exosomes by immunocapture using anti-CD3 antibodies [ 48 ].…”

Section: The Function Of Exosomes In Hnsccmentioning

confidence: 99%

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The Hidden Link of Exosomes to Head and Neck Cancer

Teng

Gao

Loveless

et al. 2021

Cancers

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Head and neck squamous cell carcinoma (HNSCC) represents an aggressive and heterogenous group of cancers whose pathologies remain largely unresolved. Despite recent advances in HNSCC therapeutic strategies, the overall survival of HNSCC patients remains poor and continues to prompt efforts to develop more effective therapies. Exosomes are a subtype of extracellular vesicles secreted by a variety of cells that have begun to spark significant interest in their roles in cancer. As membranous vesicles, spanning from 30–150 nm in diameter, exosomes mediate the transport of various molecules, such as proteins, nucleic acids, and lipids, intercellularly throughout the body. In doing so, exosomes not only act to deliver materials to cancer cells but also as signals that can confer their progression. Accumulating evidence shows the direct correlation between exosomes and the aggressiveness of HNSCC. However, more research is warranted in this field to further our understanding. In this review, we attempt to highlight the tumor-supporting roles and therapeutic potential of exosomes in HNSCC. We introduce first the biogenesis and component features of exosomes, followed by their involvement in HNSCC proliferation and metastasis. We then move on to discuss HNSCC-derived exosomes’ influence on the tumor microenvironment and their function in tumor drug resistance. Finally, we explore the promising potential of exosomes as HNSCC biomarkers and therapeutic targets and drug carriers for HNSCC treatments.

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Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs

Cited by 14 publications

References 69 publications

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

Tumour Derived Extracellular Vesicles: Challenging Target to Blunt Tumour Immune Evasion

The Hidden Link of Exosomes to Head and Neck Cancer

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