Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction

Barile, Lucio; Lionetti, Vincenzo; Cervio, Elisabetta; Matteucci, Marco; Gherghiceanu, Mihaela; Popescu, Laurențiu M.; Torre, Tiziano; Siclari, Francesco; Moccetti, Tiziano; Vassalli, Giuseppe

doi:10.1093/cvr/cvu167

Cited by 635 publications

(648 citation statements)

References 30 publications

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“…Quesenberry et al (22) reported that EVs derived from either the lung or liver entered bone marrow cells in vitro and induced expression of proteins specific for the originating lung or liver tissue. Moreover, we and others have shown that miRNA delivered via exosomes represses target genes in recipient cells (5,(23)(24)(25)(26).…”

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confidence: 74%

“…The vesicles exhibited a characteristic lipid bilayer membrane and were 50-100 nm in diameter, the size typically associated with exosomes. Fluorescently labeled human CPC exosomes were taken up by HL-1 myxoma atrial cells in vitro (5).…”

Section: Visualization Of Exosome Release From Cpcsmentioning

confidence: 99%

“…Thus, they can be viewed as cardiac-resident mesenchymal/stromal stem/ Review Article Beneficial effects of exosomes secreted by cardiac-derived progenitor cells and other cell types in myocardial ischemia progenitor cells. We have used this technique to derive these cells (5), here called cardiac-derived progenitor cells (CPCs). It should be emphasized that these cells are inclusive but not exclusively consistent of cardiac progenitor cells, as functionally defined by cardiac fate specification (6).…”

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confidence: 99%

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Beneficial effects of exosomes secreted by cardiac-derived progenitor cells and other cell types in myocardial ischemia

Barile¹,

Milano²,

Vassalli³

2017

Stem Cell Investig.

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When injected into acutely infarcted rodent or pig hearts, naturally secreted nanovesicles known as exosomes from cardiac-derived progenitor cells (CPCs) reduce scar size and improve cardiac function. In this regard, exosomes fully mimic the benefits of injecting their parent cells. This recognition paves the way to the development of exosome-based, cell-free treatments for heart disease that could possibly supplant cellbased therapies. Mechanisms of benefit of these vesicles are incompletely understood but cytoprotection, stimulation of angiogenesis, induction of antifibrotic cardiac fibroblasts, and modulation of M1/M2 polarization of macrophages infiltrating the infarcted region can all play important roles. Accordingly, the beneficial molecules carried by CPC-secreted exosomes have been identified only in part but cytoprotective and proangiogenic microRNAs (miRNA) and proteins have been described. Besides CPC-secreted exosomes, vesicles released from other cell types including mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iSPCs) have also been associated with cardioprotection. This review aims to discuss recent advances in our understanding of the role of secreted vesicles in cardiac repair, with a focus on CPC-derived exosomes.

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confidence: 74%

Section: Visualization Of Exosome Release From Cpcsmentioning

confidence: 99%

mentioning

confidence: 99%

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Beneficial effects of exosomes secreted by cardiac-derived progenitor cells and other cell types in myocardial ischemia

Barile¹,

Milano²,

Vassalli³

2017

Stem Cell Investig.

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“…Exosomes secreted from mouse MSCs contained a lot of miR-22 that exerted an antiapoptotic effect on cardiomyocytes, which restored heart function and reduced infarct size in mouse MI model [54] . Exosomes isolated from human CPCs were rich in miR-210, miR-132, and miR-146a-3p, and injection of these exosomes significantly stimulated angiogenesis, suppressed apoptosis, and improved heart function in rat MI model [55] . Therefore, these findings provide persuasive evidence that stem cell-derived exosomes exert beneficial effects on the treatment of MI in animal models.…”

Section: Stem Cell-derived Exosomesmentioning

confidence: 93%

New Strategies for Myocardial Infarction Treatment

Peng

Zhou

2017

Journal of Cardiology and Therapy

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At present, the treatments of myocardial infarction mainly focus on the recanalization of the occluded coronary artery to restore perfusion and prevent myocardial necrosis. To do this, commonly used methods include drug therapy, thrombolytic therapy, percutaneous coronary intervention (PCI), and coronary artery bypass graft (CABG) surgery. Drug therapiesTraditional drug therapies include angiotensin-converting enzyme inhibitors (ACEI), angiotensin receptor blockers (ARBs), aldosterone receptor antagonists, β-receptor blockers, and so on [1][2][3] . The main purpose of these treatments is the prevention of left ventricular remodeling. Thrombolytic therapy ABSTRACTMyocardial infarction is due to lasting and serious myocardial ischemia that leads to myocardial necrosis and cardiac remodeling, and can consequently result in heart failure. Traditional treatment for myocardial infarction includes drug therapy, thrombolytic therapy, percutaneous coronary intervention (PCI), and coronary artery bypass graft (CABG) surgery. Although these treatments can to some extent relieve the symptom of myocardial ischemia, they fail to repair the necrotic heart tissues. Myocardial regeneration is undoubtedly the best solution to the clinical problems of myocardial infarction treatment. The review briefly illustrated the pros and cons of the traditional treatments of myocardial infarction, and focused on the application of new strategies for myocardial infarction treatment using myocardial regeneration.

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“…Previous investigations have endeavored to exploitate this mechanism using cell lysates/extracts to improve cardiac function post-MI and angiogenesis. Grossman and colleagues injected bone marrow cell extracts into periinfarct myocardium using a mouse model, and several investigators have isolated microparticles and extracellular vesicles to promote neovascularization [33][34][35] . This paracrine mechanism represents a new, alternative approach to improve post-infarct cardiac regeneration and function using stem cells.…”

Section: Figurementioning

confidence: 99%

Cardiac Repair Using Stem Cells Conditioned Media Based Therapy in a Rodent Model of Myocardial Infarction and Heart Failure

Al-Refai¹,

Ridwan²,

Tarola³

et al. 2017

Canadian Journal of Cardiology

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Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction

Abstract: EVs are the active component of the paracrine secretion by human CPCs. As a cell-free approach, EVs could circumvent many of the limitations of cell transplantation.

Cited by 635 publications

References 30 publications

Beneficial effects of exosomes secreted by cardiac-derived progenitor cells and other cell types in myocardial ischemia

Beneficial effects of exosomes secreted by cardiac-derived progenitor cells and other cell types in myocardial ischemia

New Strategies for Myocardial Infarction Treatment

Cardiac Repair Using Stem Cells Conditioned Media Based Therapy in a Rodent Model of Myocardial Infarction and Heart Failure

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