Extracellular Vesicles: New Perspectives of Regenerative and Reproductive Veterinary Medicine

Zakirova, Elena; Aimaletdinov, Alexander; Malanyeva, A. G.; Rizvanov, Albert A.

doi:10.3389/fvets.2020.594044

Cited by 12 publications

(3 citation statements)

References 23 publications

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“…Fortunately, SCs can secrete extracellular vesicles (EVs) that possess the same therapeutic and regenerative effects as the parent SCs [ 29 ]. EVs are nano-vesicles that contain proteins, lipids, RNAs (mRNA, miRNA, and lncRNA), and other biomolecules that play an imperative role in paracrine signaling [ 30 , 31 ].…”

Section: Extracellular Vesicles As Alternative Therapeuticsmentioning

confidence: 99%

The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility

Qamar

Hussain

Rafique

et al. 2021

Cells

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Infertility is a globally recognized issue caused by different reproductive disorders. To date, various therapeutic approaches to restore fertility have been attempted including etiology-specific medication, hormonal therapies, surgical excisions, and assisted reproductive technologies. Although these approaches produce results, however, fertility restoration is not achieved in all cases. Advances in using stem cell (SC) therapy hold a great promise for treating infertile patients due to their abilities to self-renew, differentiate, and produce different paracrine factors to regenerate the damaged or injured cells and replenish the affected germ cells. Furthermore, SCs secrete extracellular vesicles (EVs) containing biologically active molecules including nucleic acids, lipids, and proteins. EVs are involved in various physiological and pathological processes and show promising non-cellular therapeutic uses to combat infertility. Several studies have indicated that SCs and/or their derived EVs transplantation plays a crucial role in the regeneration of different segments of the reproductive system, oocyte production, and initiation of sperm production. However, available evidence triggers the need to testify the efficacy of SC transplantation or EVs injection in resolving the infertility issues of the human population. In this review, we highlight the recent literature covering the issues of infertility in females and males, with a special focus on the possible treatments by stem cells or their derived EVs.

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Section: Extracellular Vesicles As Alternative Therapeuticsmentioning

confidence: 99%

The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility

Qamar

Hussain

Rafique

et al. 2021

Cells

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“…This technique exhibits the same regenerative potential as the original SCs but does not have the described undesirable side effects. In veterinary medicine, MV SCs are used for both regenerative and reproductive purposes [Zakirova et al, 2020b]. However, they are mainly used alongside MVs from natural origins, as opposed to artificial MVs [Qamar et al, 2021].…”

Section: Introductionmentioning

confidence: 99%

Artificial Microvesicles: New Perspective on Healing Tendon Wounds

Zakirova¹,

Aimaletdinov²,

Mansurova³

et al. 2022

Cells Tissues Organs

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Tendons have a limited capacity to repair both naturally and following clinical interventions. Damaged tissue often presents with structural and functional differences, adversely affecting animal performance, mobility, health and welfare. Advances in cell therapies have started to overcome some of these issues, however complications such as the formation of ectopic bone remain a complication of this technique. Regenerative medicine is therefore looking towards future therapies such as the introduction of microvesicles (MVs) derived from stem cells (SCs). The aim of the present study was to assess the characteristics of artificially derived MVs, from equine mesenchymal stem cells (MSCs), when delivered to rat tendon cells in vitro and damaged tendons in vivo. The initial stages of extracting MVs from equine MSCs and identifying and characterising the cultured tendon stem/progenitor cells (TSCs) from rat Achilles tendons were undertaken successfully. The horse MSCs, and the rat tendon cells, were both capable of differentiating in three directions: adipogenic, osteogenic and chondrogenic pathways. The artificially derived equine MVs successfully fused with the TSC membranes, and no cytotoxic or cytostimulating effects were observed. In addition, co-cultivation of TSCs with MVs lead to stimulation of cell proliferation and migration, and cytokine VEGF and Fractalkine expression levels were significantly increased. These experiments are the first to show that artificially derived MVs exhibited regeneration-stimulating effects in vitro, and that fusion of cytoplasmic membranes from diploid cell lines originating from different species was possible. Explorations in vivo showed accelerated regeneration of injury tendons after introduction of the MVs into damaged areas. The results from the studies performed indicated obvious positive modifying effects following the administration of MVs. This represents the initial successful steps required prior to translating this regenerative medicine technique into clinical trials, such as for tendon repair in injured horses.

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“…Recently, there has been great interest in the use of EVs from various cell types as therapeutic tools in human and veterinary medicine [ 50 , 51 , 52 , 53 ]. As concentrated platelets by means of PRP possess reparative and healing machinery, it is expected that PL-EVs might exhibit similar beneficial therapeutic properties under normal conditions due to their molecular cargo.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Therapeutic Use of Extracellular Vesicles Derived from Platelets

Špaková

Janočková

Rosocha

2021

IJMS

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Autologous blood products, such as platelet-rich plasma (PRP), are gaining increasing interest in different fields of regenerative medicine. Although growth factors, the main components of PRP, are thought to stimulate reparation processes, the exact mechanism of action and main effectors of PRP are not fully understood. Plasma contains a high amount of extracellular vesicles (EVs) produced by different cells, including anucleated platelets. Platelet-derived EVs (PL-EVs) are the most abundant type of EVs in circulation. Numerous advantages of PL-EVs, including their ability to be released locally, their ease of travel through the body, their low immunogenicity and tumourigenicity, the modulation of signal transduction as well as the ease with which they can be obtained, has attracted increased attention n. This review focuses briefly on the biological characteristics and isolation methods of PL-EVs, including exosomes derived from platelets (PL-EXOs), and their involvement in the pathology of diseases. Evidence that shows how PL-EVs can be used as a novel tool in medicine, particularly in therapeutic and regenerative medicine, is also discussed in this review.

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Extracellular Vesicles: New Perspectives of Regenerative and Reproductive Veterinary Medicine

Cited by 12 publications

References 23 publications

The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility

The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility

Artificial Microvesicles: New Perspective on Healing Tendon Wounds

Characterization and Therapeutic Use of Extracellular Vesicles Derived from Platelets

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