Extracellular Xylanopectinolytic Enzymes byBacillus subtilisADI1 from EFB’s Compost

Abstract

Microbial xylanase and pectinase are two extremely valuable enzymes, which have captivated much attention. This can be seen from the increased demand for these enzymes by many industrial sectors. This study investigates the isolation and screening of extracellular xylanopectinolytic enzymes-producing bacteria in a submerged fermentation (SmF). Samples are collected from the compost of empty fruit bunch (EFB) at Biocompost Pilot Plant, located at Biorefinery Plant, Universiti Putra Malaysia. From the experiment, out of 20 isolates, 11 isolates show xylanase or/and pectinase activity, and only one isolate (EFB-11) shows the concurrent activities of xylanase and pectinase. These activities are selected for enzyme production under submerged fermentation (quantitative screening). At the 72nd hour of incubation, xylanase and pectinase show the highest production, which ranges about 42.33 U/mL and 62.17 U/mL (with low amount of cellulase present), supplemented with 2% (w/v) of rice bran as carbon source at incubation temperature level, which is 30°C. Meanwhile, the pH of media is shifted to 8.42, which indicates that EFB-11 isolate is alkalotolerant bacteria and identified as Bacillus subtilis ADI1. This strain proves to have potential in agroindustrial bioconversion and has a promising ability to scale up to an industrial scale.

Extracellular Pectinase from a Novel BacteriumChryseobacterium indologenesStrain SD and Its Application in Fruit Juice Clarification

“…This might be due to the accumulation of waste products at prolonged incubation time with limited nutrient sources which consequently suppressed the growth of microorganism. According to Nawawi et al (2017) [ 24 ], maximum pectinase production was determined from the Bacillus subtilis ADI1 after 72 hours of incubation which well agreed with our findings.…”

“…Prakash et al (2014) [ 25 ] observed the highest production of pectinase with lactose and glucose and Jayani et al (2010) [ 26 ] reported citrus pectin as the best carbon source for pectinase production by Bacillus sphaericus. However, some researchers reported the maximum pectinase production from Bacillus subtilis ADI1 using rice brain as carbon source [ 24 ].…”

Evaluation of the Antimicrobial and Anti-inflammatory Properties of Bacillus-DFM (Norum™) in Broiler Chickens Infected With Salmonella Enteritidis

“…These findings further suggest that DFM exhibits a wide range of antibacterial activities which can be effective for both Gram-positive and negative bacteria. Although the detailed mechanism is not well-understood, these antibacterial properties of DFM might be achieved not only through competitive exclusion and production of antimicrobial peptides (AMPs), but also might be indirectly through one or several beneficial effects exhibited by them including secretion of exogenous enzymes, alternation of immunity, gut microbiota, and morphology (23, 24, 64, 65). The AMPs secreted by Bacillus sps.…”

Review on bacterial production of alkaline pectinase with special emphasis on Bacillus species

“…are also exploited for pectinase production by Bacillus spp. (Ghazala et al, 2015;Nawawi et al, 2017).…”

“…The results of a recent study indicated that out of 20 isolates screened for xylanopectinolytic enzyme activity, the most prominent strain was identifi ed as B. subtilis ADI1 (Nawawi et al, 2017). Also, pectinolytic enzyme production by B. subtilis SAV-21 was also reported in 2017 (Kaur & Gupta, 2017).…”