Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Catalano, Maria Graziella; Marano, Francesca; Rinella, Letizia; Girolamo, Laura de; Bosco, Ornella; Fortunati, Nicoletta; Berta, Laura; Frairia, Roberto

doi:10.1002/term.1922

Cited by 37 publications

(48 citation statements)

References 46 publications

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“…ESW increased BMP-2 expression, as well as ALP activity and calcium deposits with respect to untreated adipose-derived stem cells39. PCL scaffolds delivery of rhBMP-2 released in a sustained manner exhibit good cellular activity for both cell proliferation and osteogenic activity40.…”

Section: Discussionmentioning

confidence: 99%

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Chen

Huang

et al. 2017

Sci Rep

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Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our in vitro results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted in vivo, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Chen

Huang

et al. 2017

Sci Rep

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“…During wound healing, TGF-b1 can inhibit inflammation and extracellular matrix degradation because of macrophages 34. For tissue-derived stem cells, recruitment, proliferation, and differentiation may also be enhanced through ESWT treatment 35,36…”

Section: Discussionmentioning

confidence: 99%

Healing of Achilles tendon partial tear following focused shockwave: a case report and literature review

Hsu

Chang

et al. 2017

JPR

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Achilles tendinopathy is a common cause of posterior heel pain and can progress to partial tendon tear without adequate treatment. Effects of traditional treatments vary, and many recent reports focus on the use of extracorporeal shockwave therapy (ESWT) for Achilles tendinopathy but not for Achilles tendon partial tear. Here, we report the case of a 64-year-old female suffering from severe left heel pain for half a year. All treatment and rehabilitation were less effective until ESWT was applied. Each course of focused shockwave therapy included 2500 shots with energy flux density from 0.142 mJ/mm2 to 0.341 mJ/mm2. The visual analog scale decreased from nine to one degree. High-resolution musculoskeletal ultrasonography was performed before and 1 month after the treatment, which revealed healing of the torn region and decrease in inflammation. ESWT had shown to be an alternative treatment for Achilles tendon partial tear under safety procedure and ultrasound observation.

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“…34 For tissue-derived stem cells, recruitment, proliferation, and differentiation may also be enhanced through ESWT treatment. 35,36 In our case report, according to the ultrasonography performed by the same physician, healing process of partial tear can be identified. The quality of life can also become much better than before and the effect can continue for more than 4 months after the final course of therapy.…”

mentioning

confidence: 85%

Healing of Achilles Tendon Partial Tear Following Focused Shockwave: A Case Report and Literature Review

Hsu

2017

Ultrasound in Medicine & Biology

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Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Cited by 37 publications

References 46 publications

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Healing of Achilles tendon partial tear following focused shockwave: a case report and literature review

Healing of Achilles Tendon Partial Tear Following Focused Shockwave: A Case Report and Literature Review

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