Extraction and Quantification of Carpaine from Carica papaya Leaves of Vietnam

Vien, Do Thi Hoa; Loc, Tran Van

doi:10.22161/ijeab/2.5.16

Cited by 9 publications

(7 citation statements)

References 5 publications

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“…Carpaine alkaloid concentration was lower than Vien's study (2017) [28]. That study obtained 63% of carpaine alkaloid in the total papaya extract that showed concentration of carpaine carpaine alkaloid in this study was lower because of the geographical and climate differences in Indonesia and Vietnam, make the nutrients in the soil would be vary causing differences content of secondary metabolites.…”

Section: Discussioncontrasting

confidence: 53%

Isolation and Determination Carpaine Alkaloid in Papaya (Carica Papaya L.) Leaf Extract by Thin-Layer Chromatography Scanner

et al. 2021

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Objective: This research was conducted to isolate the alkaloid carpaine by chromatography method and to determine it quantitatively by Thin Layer Chromatography Scanner. Methods: Dried leaves were macerated with ethanol 70% and fractionated with dichloromethane. Isolation of carpaine alkaloid from the dichloromethane fraction was carried out by column chromatography and preparative thin-layer chromatography according to the Rf value in Thin Layer Chromatography (TLC) after exposure by Dragendorff reagent. Results: The content of carpaine alkaloid was 7.5 mg with Rf 0.58 and dichloromethane: methanol (9.2:0.8) as eluent. Validation showed the linearity (R2) 0.9988, the limit of detection(LOD) was 0.05 ppm, the Limit of Quantification (LOQ) was 0.19 ppm, the recovery from 98.93-102.43%, and the % coefficient of variation was 0.16%. Conclusion: Carpaine alkaloid in papaya leaf extract was 10.52%.

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Section: Discussioncontrasting

confidence: 53%

Isolation and Determination Carpaine Alkaloid in Papaya (Carica Papaya L.) Leaf Extract by Thin-Layer Chromatography Scanner

et al. 2021

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“…Papaya alkaloids have an anticoagulant activity that can prolong CT, PT, and aPTT times compared to controls. The total alkaloid content in papaya leaves is between 1.6% (23) and 1.8% (24) . Papaya leaf total alkaloids have antiplatelet, anticoagulant, and thrombolytic activity by increasing the percentage of inhibition of platelet aggregation, significantly prolonging CT, PT, and aPTT and increasing the percentage of thrombolytic (24) .PT increased significantly (p≤0.01) at a concentration of 100 gr and (p≤0.001) at a concentration of 300 and 600 gr, respectively.the effect of adding sample is similar to 1 mg of rivaroxaban.…”

Section: Carica Papaya Lmentioning

confidence: 99%

Review: Phytochemical of Some Plants with Anticoagulant

Sitiawani¹,

Azizah²,

Asra³

et al. 2022

Asian J Pharm Res Dev

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Background: Anticoagulants are drugs used to treat hypertension and cardiovascular disease.This review article aims to discuss several types of natural plants with anticoagulant activity. Materials and Methods: The method used is the study of relevant literature(national and international journals) which is accessed through online sites such as Google Scholar, Research Gate, Science Direct, Springer Link, and NCBI, published in the last 10 years (2011-2021) with the keyword “herbal plant” or “medicinal plant” and “anticoagulant” and “cardiovascular”. Results: Based on in vitro and in vivo tests, some plants such as Allium sativum L., Averrhoa bilimbi, Persea americana Mill., Tridax procumbens L., Carica papaya L., Rhizophora sp, Syzygiumcumini L., Piper batle L., Erigeron canadensis L., Ainsliaea fragrans, Averrhoa bilimbi, Carica papaya L., are types of plants with anticoagulant activity. Conclusion: Herb can be used as alternative sources of new anticoagulant agents cause of their biological activity. The use of medicinal plants with therapeutic effects and minimal side effects can be the best choice for the prevention and treatment of various diseases including cardiovascular disorders.

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“…Berdasarkan kajian literatur, diketahui bahwa daun pepaya mengandung alkaloid, flavonoid, fenol, asam fenolik, kolin, karposit, vitamin C dan vitamin E [31]. Pada penelitian ini, kadar alkaloid total yang diperoleh adalah 1,8%, lebih banyak dibandingkan dengan penelitian terdahulu yang hanya memperoleh 0,2% kadar alkaloid total daun pepaya [32]. Kandungan alkaloid di dalam daun pepaya ini terdiri dari alkaloid jenis karpain, pseudokarpain, emetin dan dehidrokarpain I dan II [33].…”

Section: Hasil Dan Diskusiunclassified

“…Kandungan alkaloid di dalam daun pepaya ini terdiri dari alkaloid jenis karpain, pseudokarpain, emetin dan dehidrokarpain I dan II [33]. Analisis kuantitatif karpain daun pepaya yang dimurnikan dengan HPLC menunjukkan bahwa karpain merupakan alkaloid utama dengan kandungan 63% dari total alkaloid yang diekstrak dari daun pepaya [32].…”

Section: Hasil Dan Diskusiunclassified

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Uji Aktivitas Antiplatelet, Antikoagulan, dan Trombolitik Alkaloid Total Daun Pepaya (Carica papaya L.) secara in Vitro

Rohmah

Fickri

2020

J Sains Farm Klin

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Daun pepaya memiliki kandungan beberapa senyawa alkaloid (karpain, pseudokarpain, dehydrokarpain I, dehydrokarpain II, dan emetin). Penelitian ini bertujuan untuk mengetahui aktivitas antiplatelet, antikoagulan, dan trombolitik alkaloid total daun pepaya secara in vitro. Penelitian ini terdiri dari 5 perlakuan: kontrol negatif, kontrol positif, alkaloid total daun pepaya (konsentrasi 0.5, 1.0, dan 2.0 mg/mL) dengan parameter persentasi inhibisi agregasi, persentase inhibisi koagulasi dari nilai Clotting Time (CT), Prothrombine Time (PT), dan Activated Parsial Thromboplastine Time (APTT), dan daya fibrinolitik. Berdasarkan analisis statistik diketahui bahwa terdapat perbedaan yang signifikan antara persentase inhibisi agregasi alkaloid total dibanding kontrol negative, namun tidak berbeda dengan kontrol positif (clopidogrel). Pada uji antikoagulan, alkaloid total daun pepaya secara signifikan dapat memperpanjang CT, PT, dan APTT yang berbeda signifikan dengan kontrol negatif, namun tidak berbeda dengan kontrol positif (heparin). Hasil uji trombolitik menunjukkan bahwa alkaloid total daun pepaya dapat meningkatkan persentase trombolitik yang berbeda dengan kontrol negatif, namun tidak berbeda signifikan dengan kontrol positif (nattokinase).

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Extraction and Quantification of Carpaine from Carica papaya Leaves of Vietnam

Abstract: Abstract-

Cited by 9 publications

References 5 publications

Isolation and Determination Carpaine Alkaloid in Papaya (Carica Papaya L.) Leaf Extract by Thin-Layer Chromatography Scanner

Isolation and Determination Carpaine Alkaloid in Papaya (Carica Papaya L.) Leaf Extract by Thin-Layer Chromatography Scanner

Review: Phytochemical of Some Plants with Anticoagulant

Uji Aktivitas Antiplatelet, Antikoagulan, dan Trombolitik Alkaloid Total Daun Pepaya (Carica papaya L.) secara in Vitro

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