This study was undertaken to evaluate the health-promoting potentials of Isatis aucherii, Isatis buschiana, Isatis candolleana, Isatis tinctoria subsp. corymbosa and Isatis tinctoria. I. aucherii and I. candolleana are endemic, I. buschiana, I. tinctoria subsp. corymbosa are native in Turkey. While I. tinctoria is a well studied species, there is insufficient information about other endemic and native species. Therefore, this study is focused to reveal the bioactive compounds of poorly studied endemic and native species. In this context, protein, ash, glucosinolates, fatty acids, total phenolic and flavonoid content, and antioxidant activities were determined in leaf extracts. The highest protein and fatty oil contents were observed in I. tinctoria and I. buschiana. Arachidic acid was predominant in I. tinctoria subsp. corymbosa, I. buschiana and I. aucherii, while predominant fatty acids were arachidonic and oleic acids in I. candolleana and I. tinctoria. Glucobrassicin was the main glucosinolate in I. tinctoria, while the others contained gluconapin as the main glucosinolate. Antioxidant activities were correlated with phenolic and flavonoid content, the highest and lowest antioxidant activities were observed in I. buschiana and I. aucherii, respectively. HIGHLIGHTS • I. tinctoria and I. buschiana had the highest protein and fatty oil content. • Gluconapin was the main glucosinolate in Isatis spp., except I. tinctoria. • I. buschiana was found rich in both phenolic and flavonoid compounds. • I. buschiana exhibited the highest antioxidant activity. 2 Comlekcioglu, N.