Extraction of mangiferin from Mangifera indica L. leaves using microwaveassisted<br>technique

Abstract

Mangiferin, a bioactive metabolite having potent antioxidant and pharmacological properties has been obtained in this work by microwave assisted extraction (MAE) technique, from Mangifera indica leaves. A BoxBehnken design was used to evaluate the effect of three parameters: microwave power, the time of extraction, and the ratio of solvent to raw material (mL/g) over the mangiferin yield. Irradiation time was the most important parameter on the recovery of mangiferin. The results indicated that the optimal conditions should be as follows: power of microwave 900 W, extraction time of 5 min and a solvent volume / vegetal material relation of 10. With these parameters the maximum mangiferin yield was 63.22%. The MAE process was compared with the conventional extraction in stirred tank. No significant statistical differences were found (p= 0.5639) between both extraction methods of mangiferin. The presence of the metabolite was confirmed using high performance liquid chromatography. The method was validated with the guidelines set on the International Conference on Harmonization (ICH), for the validation of analytical procedures.

Comparison of Conventional Microwave and Focused Microwave-assisted Extraction to Enhance the Efficiency of the Extraction of Antioxidant Flavonols from Jocote Pomace (Spondias purpurea L.)

“…Normally, if the extraction time is increased, the mass or quantity of metabolites extracted is increased, although sometimes there is the risk that chemical degradation may take place. The extraction of mangiferin from Mangifera indica L. leaves with MAE technique was reported by Salomon et al [32]. The concentration of mangiferin first increased at 4.5 min and then decreased at 5 min.…”

Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

“…Moreover, high antioxidant and anti-inflammatory activities of polyphenols have contributed to the protection against diabetes-related complications such as retinopathy, nephropathy, and neuropathy [47]. Notably, M. indica leaves were identified to contain various phenolic constituents, such as caffeic and gallic acids [48], mangiferin [49], flavonoids [50], and volatile compounds [51]. Especially, mangiferin, a bioactive compound, has been found as major constituent of M. indica leaves [49].…”

“…Notably, M. indica leaves were identified to contain various phenolic constituents, such as caffeic and gallic acids [48], mangiferin [49], flavonoids [50], and volatile compounds [51]. Especially, mangiferin, a bioactive compound, has been found as major constituent of M. indica leaves [49]. Therefore, mangiferin may be mainly responsible for the pharmaceutical properties of M. indica leaves, particularly anti-diabetes.…”

Ultrasonic-extraction of phenolic compounds from Phyllanthus urinaria: optimization model and antioxidant activity

“…Extraction is considering the first basic and important stage for separating bioactive compounds from raw materials (Salomon et al, 2014). Ultrasonic-assisted extraction (UAE) is one of the rapid and efficient techniques due to its strong cavitation effect and mechanical function (Liu et al, 2015b;Ma et al, 2016;Rocha et al, 2017).…”