Extractos metanólicos de corteza de guayaba (Psidium guajava L.) y mango (Mangifera indica L.): efecto citotóxico, antihemolítico y en la morfología de membrana de eritrocitos

Durán, Marlene; Castillo, Piedad Margarita Montero; Marrugo, Yesid

doi:10.31910/rudca.v16.n2.2013.904

Cited by 6 publications

(11 citation statements)

References 13 publications

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“…La obtención de eritrocitos se realizó por el Método de Boyum (1968), acorde a las modificaciones sugeridas por Durán et al (2013), mediante gradiente de densidades con Ficoll-Hypaque (Hystopaque-1077 Sigma). Posterior a la centrifugación inicial se obtuvo la capa que contenía los eritrocitos, se manejó delicadamente y a temperatura ambiente para evitar la hemólisis.…”

Section: Determinación De Actividad Hemolítica En Eritrocitosunclassified

Biological activity of Annona muricata and A. cherimola seed oils

España¹,

Ortiz²,

Eraso-Grisales³

et al. 2023

BLACPMA

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Vegetable extracts have become important raw materials for food, pharmaceutical and cosmetic industries because of their biological potential. The objective of this study was toassess the biological activity of vegetable oils (VOs) extracted from Annona muricata and A. cherimola. Antibacterial activity was determined by plaque microdilution. The assessment of hemolytic inhibition and morphological alterations was performed in erythrocyte cultures by spectrophotometry and microscopy, respectively. Neutrophils were used to analyze both cytotoxicity by the trypan blue exclusion method and the effect on gelatinase granule release (MMP9) via zymography. Whereas VOs showed a mild antibacterial activity (900 μL/mL) on five ATCC bacterial strains, they had no effect on multi-resistant bacteria. In addition, VOs inhibited hydrogen peroxide induced hemolysis and did not cause erythrocyte cell abnormalities. Cytotoxicity was not detected in neutrophils and VOs were able to stimulate MMP9 release. These results support their potential use by the food and cosmetic industries due to their antioxidant, non-cytotoxic, and slight antibacterial capacities

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Section: Determinación De Actividad Hemolítica En Eritrocitosunclassified

Biological activity of Annona muricata and A. cherimola seed oils

España¹,

Ortiz²,

Eraso-Grisales³

et al. 2023

BLACPMA

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“…Los eritrocitos pueden cambiar su forma normal a la forma de equinocitos o estomatocitos, lo cual depende de factores citoplasmáticos, entre ellos el pH (Gedde et al, 1997). Las altas concentraciones de flavonoides hallados en frutas como el mango (García Bacallao et al, 2001), hojas de guayaba (Rodríguez et al, 2013) y flavonoides obtenidos de maqui (Aristotelia chilensis) con propiedades antioxidantes, son inductores de equinocitos (Gironés-Vilaplana et al, 2012;Durán et al, 2013). Con el uso del CHROMagar se identificó E. coli O:157 H7, diferenciándose por el color de la colonia en el medio, presenta una coloración rosa malva (Lara et al, 2019).…”

Section: Extracto De Hoja De Guayaba Carvacrol Timolunclassified

Caracterización química de extracto alcohólico de hoja de guayaba (Psidium guajava) y su efecto como inhibidor de movilidad para Escherichia coli O157:H7

et al. 2020

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“…The values obtained with FRAP and ORAC reflect the overall capacity or antioxidant activity of the samples evaluated. These are two methodologies that have been implemented in recent years due to their great sensitivity and precision [70,71], which makes it possible to post the agroindustrial residues of gulupa as a source of antioxidant components. Phenolic compounds and alkaloids are some of the metabolites recognized for their antioxidant action against these evaluations [72].…”

Section: Antiradical and Antioxidant Capacitymentioning

confidence: 99%

Potential Uses of the Peel and Seed of Passiflora Edulis Sims F. Edulis (Gulupa) From Its Chemical Characterization, Antioxidant, and Antihypertensive Functionalities

GONZÁLEZ

Álvarez

Murillo

et al. 2019

Asian J Pharm Clin Res

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Objective: Assess the performance of a crude ethanolic extract, a dichloromethane fraction and a hydroalcoholic residue, which are the basis for chemically and biologically characterizing the husk and seed of Passiflora edulis f. edulis, collected in the region and Colombia with a view to determining potential uses. Methods: Agroindustrial residues of gulupa (peel and seed) were analyzed through a bromatological study; subsequently, they were macerated with ethanol (96%). The crude ethanolic extract was partitioned with dichloromethane, leaving a hydroalcoholic residue. The content of total phenols, the composition of phytophenols (high-performance liquid chromatography-mass spectrometry), the total antioxidant capacity using 3-ethyl benzothiazoline-6-sulfonic acid (ABTS●+) and 2,2-diphenyl-1-pyridyl hydrazyl (DPPH●), the oxygen radical absorbance capacity (ORAC), and the ferric reduction power (FRAP) were determined to the extract, the fraction, and the residue. The evaluation of the inhibitory activity of the angiotensin-converting enzyme inhibitor (ACEI) and the cell viability assay with diphenyl bromide 3- (4,5-dimethylthiazole-2-) il) -2,5-tetrazolium on human leukocytes complemented the characterization. Results: Agroindustrial waste of P. edulis f. edulis, peel and seed, contains as main constituents: Protein (8.49 and 7.29%), fiber (34.2 and 55.7%), phosphorus (1.67 and 3.09), and boron (53.3 and 58.4 mg/kg), respectively. The seed showed 25.5% oil. The crude seed extract exhibited a higher phenolic content (15.34 gEAG/100 g). Likewise, it presented the highest antiradical capacity against ABTS●+ and DPPH● (706.17 and 82.81 trolox equivalent antioxidant capacity [TEAC], respectively) and antioxidant in ORAC and FRAP (142.79 TEAC and 103.63 equivalent ascorbic acid EAA, respectively). The ACEI activity (50% inhibitory concentration 17.62 mg/L) of the crude seed extract was higher than the other samples. No toxicity was found in the samples evaluated at concentrations higher than those of the biological activities manifested. Conclusion: The agroindustrial residues of P. edulis f. edulis (peel and seed) are rich in nutrients, which propose them for use in food matrices. The ethanolic extract from seed showed the highest antioxidant, antiradical, and inhibitory biological activity of the ACEI so that it could be proposed the gulupa seed as a promising phytotherapeutic product associated with its phenolic content, especially its flavonoids. The results obtained allow an added value to the fruit, reducing the chances that its waste contributes to environmental pollution.

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Extractos metanólicos de corteza de guayaba (Psidium guajava L.) y mango (Mangifera indica L.): efecto citotóxico, antihemolítico y en la morfología de membrana de eritrocitos

Cited by 6 publications

References 13 publications

Biological activity of Annona muricata and A. cherimola seed oils

Biological activity of Annona muricata and A. cherimola seed oils

Caracterización química de extracto alcohólico de hoja de guayaba (Psidium guajava) y su efecto como inhibidor de movilidad para Escherichia coli O157:H7

Potential Uses of the Peel and Seed of Passiflora Edulis Sims F. Edulis (Gulupa) From Its Chemical Characterization, Antioxidant, and Antihypertensive Functionalities

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