Extrusion Bioprinting

Pati, Falguni; Jang, Jinah; Lee, Jin Woo; Cho, Dong‐Woo

doi:10.1016/b978-0-12-800972-7.00007-4

Cited by 102 publications

(76 citation statements)

References 143 publications

(184 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, the materials should provide a biocompatible environment as well as an adequate mechanical support for cell viability and function. 42 In our work, we employed a complex hydrogel consisting of three natural biomaterials, gelatin, alginate and carboxymethyl chitosan. Here, the gelatin provides the appropriate viscous and temperature-dependent crosslinking velocity that can promote the complex hydrogel gelation quickly at 15 C during printing.…”

Section: Printed Cell-laden Hydrogel Scaffoldsmentioning

confidence: 99%

BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting

Huang

Wang

et al. 2016

RSC Adv.

View full text Add to dashboard Cite

Three-dimensional (3D) bioprinting technology offers the possibility to deliver, in a defined and organized manner, scaffolding materials and living cells, and therefore holds much promise for tissue engineering and regenerative medicine. In this work, we explored the possibility of gelatin/sodium alginate/carboxymethyl chitosan (Gel/SA/CMCS) hydrogel combining with bone mesenchymal stem cells (BMSCs) for 3Dbioprinting. Compared to the commonly used 3D bioprinting materials such as gelatin/sodium alginate (Gel/SA) hydrogel, the Gel/SA/CMCS hydrogel we developed shows excellent equilibrium water content, good mechanical properties, antibacterial activity, and a slow degradation rate. With this kind of material, we generated a scaffold with homogenous cell distribution. Cell viability after 3D printing showed that over 85% of the printed cells were viable at 0 and 2 days of culturing. Our work demonstrates the feasibility of mixing Gel/SA/CMCS hydrogel with stem cells for 3D bioprinting.

show abstract

Section: Printed Cell-laden Hydrogel Scaffoldsmentioning

confidence: 99%

BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting

Huang

Wang

et al. 2016

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…All of these techniques present different principles, characteristics, advantages, and disadvantages already described in detail elsewhere (Murphy and Atala, 2014;Seol et al, 2014;Mandrycky et al, 2016;Bishop et al, 2017;Peng et al, 2017;Jang et al, 2018). Briefly, the most common bioprinting technique is microextrusion (Figure 5A), which consists of a fluid-dispensing system that uses pneumatic pressure or mechanical forces (piston or screw) to print a continuous filament through a nozzle (Pati et al, 2015;Mandrycky et al, 2016;Ozbolat and Hospodiuk, 2016). Microextrusion allows the bioprinting of high viscosity bioinks (30 to >6 × 107 mPa/s), a wide selection of biomaterials, and very high cell densities (Murphy and Atala, 2014;Mandrycky et al, 2016).…”

Section: D In Situ Bioprintingmentioning

confidence: 99%

In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments

Dias

Ribeiro

Baptista-Silva

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

“…Inkjet printing is a non-contact method of fabrication, which limits the risk of contamination during printing. Scaffolds are constructed using the deposition of droplets and can achieve excellent resolution ranging between 20 and 100 μm [9][10][11]. Droplets are ejected from a printhead via thermal, piezoelectric or microvalve processes.…”

Section: Printing Techniques 21 Inkjet Printingmentioning

confidence: 99%

“…Bioink is extruded from a micro-nozzle to build a three-dimensional structure in layer-by-layer fashion. Extrusion-based printing is either mechanically (piston or screw) or pneumatically driven [9]. This fabrication technique uses highly viscous bioink and does not require any chemical additives to enable the curing of the material [9].…”

Section: Extrusionmentioning

confidence: 99%

Bioprinting

Rider¹,

Kačarević²,

Retnasingh³

et al. 2019

Biomaterial-Supported Tissue Reconstruction or Regeneration

View full text Add to dashboard Cite

Bioprinting is an emerging field in the areas of tissue engineering and regenerative medicine. It is defined as the printing of structures consisting of living cells, biomaterials and active biomolecules. The ultimate aim is to produce implantable organs and tissues to replace the use of autografts, which cause donor site morbidity and require two invasive surgeries. Not only is bioprinting aimed at the restoration of tissue, it has significant potential for drug delivery and cancer studies. Bioprinting provides control over cell placement and therefore creates a homogenous distribution of cells correlating to a uniform tissue ingrowth. Another attribute of bioprinting is the production of patient-specific spatial geometry, controllable microstructures and a high degree of reproducibility and scalability between designs. This book chapter will discuss the many parameters of bioprinting; manufacturing techniques, precursor materials, types of printed cells and the current research.

show abstract

Extrusion Bioprinting

Cited by 102 publications

References 143 publications

BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting

BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting

In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments

Bioprinting

Contact Info

Product

Resources

About