Eye Platelet-Rich Plasma (E-PRP) for Corneal Regeneration

Rodrı́guez, Alejandra; Alió, Jorge L.

doi:10.1007/978-3-030-01304-2_22

Cited by 3 publications

(12 citation statements)

References 149 publications

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“…Complete corneal healing was achieved at 3.62±0.23 days. This results matches with other authors claiming that E-PRP contains higher GFs than obtained from AS ( more reference needed) (Rodríguez and Alió 2019).…”

Section: Discussionsupporting

confidence: 92%

“…Eye drops preparation E-PRP preparation was carried out under strict sterile conditions using sterile and disposable materials at the laboratory of faculty of veterinary medicine Sadat City University. Blood sample collection from jugular vein 10 ml, then E-PRP preparation method as prepared previously in 2019 for human (Rodríguez and Alió 2019). technique and methods of preservation as performed as described by in human (Kim, et al, 2012) Autologous serum was prepared by spontaneous coagulation of the blood extracted without anticoagulant in a test tube.…”

Section: Animalsmentioning

confidence: 99%

“…They found that a significant higher concentration of TGF-β1 and EGF, PDGF and Fibronectin in E-PRP than found in AS. Moreover, E-PRP induces a significantly higher epithelization ratio promoting the rearrangement of actin filaments stabilizing the tear film and prevent apoptosis (Kim, et al, 2012, Rodríguez andAlió 2019). Although there isn`t activation of platelets in E-PRP, its efficacy didn`t depend on platelets activation due to plasma contain growth factors, plasma protein, and epitheliotrophic factors that accelerate corneal wound healing, Indicating a positive and significant correlation between PDGF-BB and platelets in E-PRP (Rodriguez, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Compare the Efficacy of Corneal Healing Between AS and E-PRP Treatment in Dogs

Gadallah

Misk

Ghaith

et al. 2021

Journal of Current Veterinary Research

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Corneal ulcer is considered one of the most common ocular surface diseases, it may lead to blepharospasm, photophobia, lacrimation, conjunctival hyperemia, and corneal oedema. Conventional treatments are sometimes not effective in solving the pathological condition therefore blood derivatives which are rich in growth factors were used to accelerate wound healing as well as its ability to induce regeneration of affected tissues. Blood derivatives include autologous serum (AS), eye drops platelets rich plasma (E-PRP). A 6 mm induced corneal wound were performed at the center of the cornea at one eye at a 240 M depth in 20 mongrel dogs. Dogs divided into three groups, E-PRP group (8 dogs) and AS group (8 dogs) and a control group (4 dogs) received normal saline eye drops. All dogs were received 4-time treatment per day until negative fluorescein stain test is obtained. Histological study was performed for 3 dogs (one from each group) two months from the beginning of the experiment. Clinically the E-PRP group showed faster healing time with a significant difference (3.62±0.23 day) from AS group (4.75±0.25 day) and control group (6.75±0.30 day). Histologically, E-PRP group showed more arrangement of the epithelial layers compared with AS group and control group. Aim of our study: to compare the efficacy of AS and E-PRP in the healing of induced corneal wound in dogs. In conclusion, E-PRP and AS showed a significant faster epithelial healing from control group. As well as E-PRP showed also a significant improvement over AS in heling time and quality of regenerated epithelial tissue.

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Section: Discussionsupporting

confidence: 92%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Compare the Efficacy of Corneal Healing Between AS and E-PRP Treatment in Dogs

Gadallah

Misk

Ghaith

et al. 2021

Journal of Current Veterinary Research

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“…PDGF-BB-induced tissue repair mechanisms appear to involve fibroblast proliferation, collagen production and new vessel formation and may involve direct effects on mesenchymal cells expressing PDGFR-α. The E-PRP is a 100% autologous compound since it does not contain any additives (Rodríguez and Alió 2019). E-PRP for ophthalmological use is carried out through a one-step centrifugation process.…”

Section: Surgerymentioning

confidence: 99%

“…Concentrated GF lead to accelerated endothelial and epithelial regeneration and stimulate angiogenesis, collagen synthesis, soft-tissue healing, and homeostasis (Jee, et al, 2016). PDGF-BBinduced tissue repair mechanisms appear to involve fibroblast proliferation, collagen production and new vessel formation and may involve direct effects on mesenchymal cells expressing PDGFR-α (Rodríguez and Alió 2019). Now-a-days, E-PRP is being used in treatment of more than 30 diseases including tissue engineering and research, cardio-vascular surgery, gastroenterology, maxillofacial,…”

Section: Introductionmentioning

confidence: 99%

Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs

Ahmed

Misk

Gadallah

et al. 2021

Journal of Current Veterinary Research

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Several studies demonstrate the effectiveness of eye drops Platelet Rich Plasma (E-PRP) as a promoter of the healing of different ophthalmological disorders in human, due to the high concentration of growth factors as EGF, PDGF-BB and Fibronectin which represent the key to obtain the desired regenerative effects on the ocular surface. There is different protocol for PRP eye drops preparation for use in human corneal disorders. This study was to evaluate an easy and affordable technique for preparation of canine autologous E-PRP according to standard measure used before for human corneal disorders to be used in canine corneal ulcers in comparison with control group, all treatments were applied four times daily. Twelve healthy dogs were divided into two groups first group included eight dogs which received autologous E-PRP compared with second group which included four dogs and received saline eye drops as control. Complete blood count is performed to whole blood and E-PRP to validate last one content of platelets which must equal more 1.5 those in whole blood. E-PRP contain platelets 2.41-fold than those in whole blood with one step centrifugation technique.Autologous canine E-PRP mean healing time was 3.62±0.23 days while with control group mean healing time was 6.75±0.30 days. In conclusion, As the E-PRP is now widely documented in human ophthalmology, it had been proven as a great result in veterinary practice when prepare autologous E-PRP by single step centrifugation to be used for canine corneal wound and healing rate was better than control group by significant difference. which make it novel option for treatment for canine corneal ulcer.

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Autologous Platelet-Rich Plasma in The Management of Diabetic Foot Ulcer: A Systematic Review of Randomized Controlled Trials

2022

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Context: Diabetic foot ulcer (DFU) remains a devastating and prevalent complication of diabetes, which leads to significant morbidity, mortality, and economic implications. Outcomes of DFU management are still unsatisfactory, warranting innovations to improve them. One accessible and promising potential for treating DFU is autologous platelet-rich plasma (PRP), a blood product abundant in bioactive molecules. Platelet-rich plasma has been used in other fields of medicine to treat various conditions. Evidence Acquisition: While there is substantial research studying the use of PRP for DFU, these studies are regarded as having flaws in methodology. Hence, this systematic review aims to bridge the knowledge gap by only including randomized controlled trials (RCT). Results: After sifting through various databases, six studies were included. This review focused on specific outcomes, namely percentage of patients with healed wounds, wound size, and time to closure. Conclusions: The authors found that PRP has performed well with regards to healing DFU wounds with a high rate of healing, which leads to a shorter time to wound closure.

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Eye Platelet-Rich Plasma (E-PRP) for Corneal Regeneration

Cited by 3 publications

References 149 publications

Compare the Efficacy of Corneal Healing Between AS and E-PRP Treatment in Dogs

Compare the Efficacy of Corneal Healing Between AS and E-PRP Treatment in Dogs

Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs

Autologous Platelet-Rich Plasma in The Management of Diabetic Foot Ulcer: A Systematic Review of Randomized Controlled Trials

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