The microstructural homogeneity across thickness and the interior toughness are significant factors challenging the development of ultra-heavy steel plate (above 200 mm) limited by the thermal transfer capacity of material and the loading capacity of mill. A complete understanding of the relationship between processing, structure, and property is essential for the optimization of alloying and heat treatment of ultra-heavy steel plate. Hence, in the present work, the authors focus on the microstructural evolution and resultant mechanical properties across the thickness of ultra-heavy steel plate under three potential heat treatment technologies, including quenching and tempering, double quenching and tempering, and intercritical quenching and tempering. The results show that the refined signal-phase lath martensite microstructure after double quenching technology can improve toughness stability, while dual-phase intercritical ferrite-martensite microstructure resulted from intercritical quenching technology achieves excellent toughness stability and ductility, and a potential optimal combination of strength and toughness for ultra-heavy steel plate. However, limited cooling capacity of the interior leads to the formation of massive coarse martensite/austenite constituents with high carbon concentration, which significantly deteriorate the toughness. Thus, the control or suppression of coarse martensite/austenite constituents is critical for optimization of lowalloyed ultra-heavy steel plate.