Fabrication and application of carbohydrate microarray for analyzing human serum antibody–carbohydrate interaction

Abstract

We introduced a strategy for preparing a carbohydrate microarray and demonstrated its utility for characterizing carbohydrate binding and activities. We isolated the lipopolysaccharide (LPS) components from different bacteria and explored the possibility of immobilizing these glycoconjugates on a high-binding polystyrene plate. Carbohydrate-specific combination was examined by observing the binding of the blood group B analogic LPS O-polysaccharide from Escherichia coli on the high-binding polystyrene plate and anti-B from a broad spectra antibody of human blood serum. Strong binding of antibodies was screened, as it was evident that relative response value is two times higher than control. The hybridization results indicated that this method is a reliable technique for the detection of human intestinal bacteria and is expected to be applied in diagnostics and seroepidemiology.

Use of Microarrays as a High-Throughput Platform for Label-Free Biosensing

“…They used this microarray to analyze specific glycan-binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses. 114,115 Carbohydrate microarrays have also been used to profile the specificity of influenza virus receptors 116,117 and human serum antibodies 118,119 for the purpose of developing new carbohydrate-based vaccines and drugs.…”