Improvement the tolerance of baker's yeast to toxic substrate/product with cloud point system during the whole cell microbial transformation

Wang, Zhilong; Xu, Jian‐He; Wang, Li; Zhang, Wenzhi; Zhuang, Baohua; Qi, Haifeng

doi:10.1016/j.enzmictec.2007.02.009

Cited by 15 publications

(6 citation statements)

References 38 publications

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“…2. The dispersible aqueous systems are toxic to the microorganism Saccharomyces cerevisiae and the biomass of S. cerevisiae growing in the series of nonionic surfactant aqueous systems is also consistent with the phase separation [50]. Polyethylene glycol (PEG) induces a Triton X-100 aqueous solution to form a novel cloud point system is shown in Fig.…”

Section: Biocompatibilitymentioning

confidence: 57%

“…The cloud point system has been improved them to 0.6 and 0.7% (v/v) in the cloud point system, respectively. And reutilization of the whole cell microorganism as biocatalyst indicates that it maintains its biocatalytic activity at least four times [50].…”

Section: Microbial Transformation Of Sterolsmentioning

confidence: 99%

“…b Bioreduction involving cofactor NAD (P) H regeneration with viable cells of Saccharomyces cerevisiae [50]. c Precursor fermentation involving glucose metabolism by Saccharomyces cerevisiae to produce pyruvic acid as one of its substrates [ Triton X-114 and Triton X-100 Mycobacterium sp (resting cell) Cholesterol ADD [64] Triton X-114 and Triton X-100 Mycobacterium sp (resting cell or growing cell) Phytosterol ADD [65] Triton X-45 and Triton X-114 Saccharomyces cerevisiae (growing cell and resting cell) Acetophenone 1-phenylethanol [50] Triton X-100 and PEG 20000 Saccharomyces cerevisiae (resting cell) Benzaldehyde L-PAC [66] be further validated by more model reactions. A deep insight into the mechanism behinds the phenomenon will be a major goal in the future research.…”

Section: Future Trendsmentioning

confidence: 99%

“…The wet cell weight (WCW) is determined by Saccharomyces cerevisiae growing in a culture medium containing 10% of nonionic surfactant for 3 days. The blank bar is WCW; the Wlled circle is the cloud point and a phase separation occurred under culture temperature; the open circle is the cloud point above culture temperature and the surfactant is dispersible under the culture temperature[50] …”

mentioning

confidence: 99%

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Whole cell microbial transformation in cloud point system

Wang

Chen³

2008

J Ind Microbiol Biotechnol

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Cloud point system, consisting of nonionic surfactant in an aqueous solution, has been developed as a novel medium for whole cell microbial transformation. The basic properties of cloud point system including phase separation and solubilization are introduced. The application of cloud point system for extractive microbial transformation is different from that of water-organic solvent two-phase partitioning system or aqueous two-phase system are discussed, which mainly focus on the biocompatibility of microorganism in a cloud point system and a downstream process of microbial transformation in cloud point system with oil-water-surfactant microemulsion liquid-liquid extraction for surfactant recovery and product separation. Finally, examples of whole cell microbial transformation in cloud point systems, especially in situ extraction of moderate polar substrate/product, are also presented.

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Section: Biocompatibilitymentioning

confidence: 57%

Section: Microbial Transformation Of Sterolsmentioning

confidence: 99%

Section: Future Trendsmentioning

confidence: 99%

mentioning

confidence: 99%

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Whole cell microbial transformation in cloud point system

Wang

Chen³

2008

J Ind Microbiol Biotechnol

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“…Therefore, further studies are needed to enhance the efficiency of biocatalyst for the production of chiral building blocks. A lot of effort has been spent on the bottleneck in the one‐step reaction, such as reduction reactions of ketones, Baeyer–Villiger oxidation and resolution of eoxide, however, only few studies have been published on microbial stereoinversion involving tandem oxidation–reduction reactions 14–17. As a result, the methods for improving the efficiency of biocatalysts have rarely been reported 18…”

Section: Introductionmentioning

confidence: 99%

An efficient strategy overcoming the bottleneck in Candida parapsilosis catalyzing stereoinversion from (R)‐1‐phenyl‐1, 2‐ethanediol to the corresponding counterpart

Nie

2009

J of Chemical Tech & Biotech

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BACKGROUND: Microbial stereoinversion has been widely used for the biosynthesis of numerous chiral compounds. However, little work has been done to improve the efficiency of microbial stereoinversion. This study investigated the bottleneck in the deracemization of 1-phenyl-1,2-ethanediol (PED), and then the efficiency and the sustainability of biocatalyst was improved significantly by using a strategy.

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Bioreduction

Parachin¹,

Carlquist²,

Gorwa-Grauslund³

2010

Encyclopedia of Industrial Biotechnology

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Bioreduction has emerged over the years as an alternative method to organic synthesis for the generation of chiral precursors of commercial interest. Bioreductions operate under mild conditions of pH and temperature with the help of highly regio‐ and enantio‐selective oxidoreductase enzymes. In this contribution, the different oxidoreductase families involved in bioreductions are exemplified and their main characteristics are presented. The wide spectrum of oxidoreductase substrates (including ketones, diketones, ketoesters, aldehydes, alkenes, and keto acids) is discussed and both preparative and industrial scale examples are reported. The advantages and disadvantages of using isolated enzymatic systems versus whole‐cell systems for bioreduction are discussed in terms of cost, specificity, stereoselectivity, and cofactor regeneration. The contribution is also reviewing strategies for improving the biocatalyst at the cell or enzyme level, which include process engineering, metabolic engineering as well as structure‐based and nonstructure‐based enzyme engineering. Finally, the potential role of metagenomics for isolating novel biocatalysts from different environments is discussed.

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Improvement the tolerance of baker's yeast to toxic substrate/product with cloud point system during the whole cell microbial transformation

Cited by 15 publications

References 38 publications

Whole cell microbial transformation in cloud point system

Whole cell microbial transformation in cloud point system

An efficient strategy overcoming the bottleneck in Candida parapsilosis catalyzing stereoinversion from (R)‐1‐phenyl‐1, 2‐ethanediol to the corresponding counterpart

Bioreduction

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