Fabrication and Appraisal of Poly (Lactic-Co-Glycolic Acid) – Moxifloxacin Nanoparticles using Vitamin E-TPGS: A Potential Intracanal Drug Delivery Agent

Makkar, Hardik

doi:10.7860/jcdr/2017/27633.9957

Cited by 12 publications

(13 citation statements)

References 26 publications

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“…Ideally, such medicaments should deliver and maintain significant concentrations of antimicrobial agent during the time period between RCT sessions; this is the role that the sustained-release formulations can readily play. Sustained-release delivery systems proposed for intracanal use range from preformed devices loaded with CHX [34, 40] to systems comprising micro- and nanoparticles [41, 42]. The formulation of SRF tested here contains Eudragit® RL polymers in approved solvents that provide good injectability into the root canal.…”

Section: Discussionmentioning

confidence: 99%

Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study

Funk

Sahar-Helft

Kirmayer

et al. 2019

International Journal of Dentistry

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Enterococcus faecalis is the most commonly recovered species from failed root canal treatments. In this study, we tested the capability of a novel intracanal sustained-release filler (SRF) containing cetylpyridinium chloride (CPC) to disinfect dentinal tubules of segmented human tooth specimens. Human dental root specimens were infected with E. faecalis V583 for 3 weeks in a static environment. The tested intracanal medicaments were SRF-CPC and calcium hydroxide (CH). Each medicament was introduced into the canal of the dental specimen and incubated for 7 days. The bacteriological samples were taken by shaving the dentine surrounding the root canal with dental burs ranging in size from ISO 014-020. The obtained dentine powder was collected in test tubes containing phosphate-buffered saline, sonicated, and plated on agar plates. Colony-forming units were counted after 48 h of incubation. Random specimens were also examined under confocal laser scanning microscopy and scanning electron microscopy. A statistical difference was found in the bacterial counts obtained from all layers of infected dentin between the control and the SRF-CPC groups. CH reduced bacterial viability significantly only in the first layer of the infected dentin, up to 150 μm into the dentinal tubules. CLSM images showed that SRF-CPC killed most bacteria throughout the infected dentin up to 700 μm of penetration. SEM images demonstrated the adhesion ability of SRF-CPC to the dentinal wall. In conclusion, SRF-CPC is a potential intracanal medicament for disinfecting dentinal tubules.

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Section: Discussionmentioning

confidence: 99%

Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study

Funk

Sahar-Helft

Kirmayer

et al. 2019

International Journal of Dentistry

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“…Mesoporous calcium-silicate nanoparticles loaded with CHX had excellent antibacterial and in vitro mineralization properties [47]. PLGA-moxifloxacin nanoparticles were also shown to be effective against E. faecalis for at least 14 days [48]. However, concerns about the potential toxicity of nanoparticles have been voiced in the literature, as the interactions at the nano-bio interface may impact the function of biomolecules, cellular components and tissue structures, well beyond the contemplated drug delivery paradigm [49].…”

Section: Discussionmentioning

confidence: 99%

Efficacy and potential use of novel sustained release fillers as intracanal medicaments against Enterococcus faecalis biofilm in vitro

et al. 2019

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Background Enterococcus faecalis is a bacterium frequently isolated after failed root canal therapy. This study analyzed the antibacterial and antibiofilm effects in vitro of sustained-release fillers (SRF) containing cetylpyridinium chloride (CPC) against vancomycin resistant E. faecalis . Methods First, the solidification capability was tested by introducing liquid SRF into phosphate buffered saline, followed by 30 s of vortexing. The antimicrobial effects of SRF-CPC against static monospecies biofilms were analyzed with a metabolic assay. Inhibition of biofilm formation was tested by exposing daily refreshed E. faecalis suspensions to SRF-CPC for 9 weeks. To evaluate the effects of SRF-CPC against preformed biofilms, biofilms were grown for 1, 3 and 7 days, and then treated with SRF-CPC for 24 h. Biofilm kill time was tested by applying SRF-CPC to a 3-day-old biofilm and measuring its viability at different time points. All experiments were compared to Placebo SRFs and to untreated control biofilms. Data were analyzed with two-way ANOVA followed by Tukey’s test. Results were considered significant at P < 0.05. Results The liquid SRF solidified within seconds and no structural changes were observed after 30 s of vortexing at maximum speed. SRF-CPC inhibited E. faecalis biofilm formation for 7 weeks and significantly reduced its viability in weeks 8 and 9. Mature biofilms grown for 1, 3 and 7 days were destructed by SRF-CPC in less than 24 h. Fifty percent of a 3-day-old biofilm was destructed in 2 h and complete destruction occurred in less than 12 h. ( P < 0.05 in all cases, compared to SRII-Placebo). Conclusions SRF-CPC’s physical properties and long-lasting anti-biofilm effects make it a promising coadjuvant medication for endodontic therapy. Electronic supplementary material The online version of this article (10.1186/s12903-019-0879-1) contains supplementary material, which is available to authorized users.

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“… 101 A study compared the antimicrobial efficacy of nanoparticulate chitosan and poly(lactic-co-glycolic) acid (PLGA) employed as potential intracanal antibiotic delivery agents, with the latter exhibiting better sustained antimicrobial effects over 2 weeks. 107 …”

Section: Intracanal Medicamentsmentioning

confidence: 99%

“… 177 As nanoparticles possess enhanced solubility, high surface-area-to-volume ratio and minute dimensions, nanoparticle-based carrier systems may improve the dissolution and absorption of bioactive molecules and drugs. 176 Several polymeric nanocarriers have been investigated in the context of conventional root canal treatment, such as those previously discussed including chitosan, PLGA and PolymP- n Active nanoparticles, 107 , 165 , 166 as well as regenerative therapies. 178–182 Bovine serum albumin-loaded chitosan nanoparticles were found to improve the viability of stem cells from the apical papilla (SCAP) and enhance alkaline phosphatase activity.…”

Section: Regenerative Endodontic Strategiesmentioning

confidence: 99%

The Potential Translational Applications of Nanoparticles in Endodontics

Wong

Zou

Lee

et al. 2021

IJN

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Nanotechnology has substantially progressed in the past decades, giving rise to numerous possible applications in different biomedical fields. In particular, the use of nanoparticles in endodontics has generated significant interest due to their unique characteristics. As a result of their nanoscale dimensions, nanoparticles possess several properties that may enhance the treatment of endodontic infections, such as heightened antibacterial activity, increased reactivity and the capacity to be functionalized with other reactive compounds. Effective disinfection and sealing of the root canal system are the hallmarks for successful endodontic treatment. However, the presence of bacterial biofilms and resistance to endodontic disinfectants pose a significant challenge to this goal. This has encouraged the investigation of antibacterial nanoparticle-based irrigants and intracanal medicaments, which may improve the elimination of endodontic infections. In addition, photosynthesizer-functionalized nanoparticles could also serve as a worthy adjunct to root canal disinfection strategies. Furthermore, despite the myriad of commercially available options for endodontic obturation, the “ideal” material has yet to be conceived. This has led to the development of various experimental nanoparticle-incorporated obturation materials and sealers that exhibit a range of favourable physicochemical properties including enhanced antibacterial efficacy and bioactivity. Nanoparticle applications also show promise in the field of regenerative endodontics, such as supporting the release of bioactive molecules and enhancing the biophysical properties of scaffolds. Given the constantly growing body of research in this field, this article aims to present an overview of the current evidence pertaining to the potential translational applications of nanoparticles in endodontics.

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Fabrication and Appraisal of Poly (Lactic-Co-Glycolic Acid) – Moxifloxacin Nanoparticles using Vitamin E-TPGS: A Potential Intracanal Drug Delivery Agent

Cited by 12 publications

References 26 publications

Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study

Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study

Efficacy and potential use of novel sustained release fillers as intracanal medicaments against Enterococcus faecalis biofilm in vitro

The Potential Translational Applications of Nanoparticles in Endodontics

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