Fabrication and characteristics of microcantilever-based biosensor for detection of the protein-ligand binding

Yoo, Kyung-Ah; Kim, Junhyuk; Nahm, Baek Hie; Kang, Chi Jung; Kim, Yong−Sang

doi:10.1088/1742-6596/61/1/258

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…RCA (Radio Corporation of America) is a standard wafer cleaning procedure used in microelectronics industry to remove the metal contamination and organic impurities. 23 Thereaer, ppolysilicon piezo-resistive layer of thickness 50 nm was deposited by LPCVD (Low Pressure Chemical Vapour Deposition) process 24,25 and patterned using a standard micro fabrication process. In order to dene the electrical contacts, Cr/Au of thickness 20/200 nm respectively were sputtered and patterned.…”

Section: Experimental Methodsmentioning

confidence: 99%

Asymmetric immobilization of antibodies on a piezo-resistive micro-cantilever surface

et al. 2016

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Section: Experimental Methodsmentioning

confidence: 99%

Asymmetric immobilization of antibodies on a piezo-resistive micro-cantilever surface

et al. 2016

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“…In 2004, Savran et al [ 153 ] modified the aptamer probe on the working cantilever surface while there was a nonspecific oligonucleotide probe on the reference cantilever, and successfully recognized specific proteins containing thrombin and Taq DNA polymerase. In 2007, Yoo et al [ 154 ] modified an avidin-sensitive membrane on the surface of a microcantilever by self-assembly and monitored in real time the binding process of a streptavidin ligand. In 2010, Wang et al [ 155 ] used a microcantilever modified by a platelet-derived growth factor (PDGF) aptamer probe to quantitatively study the effect of temperature on the binding process between the probe and PDGF.…”

Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.

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Fundamentals and current status of polymeric piezoresistive cantilever technology applied on biosensors

Fraga,

Chiappim,

Pessoa

2024

Semiconducting Polymer Materials for Biosensing Applications

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Fabrication and characteristics of microcantilever-based biosensor for detection of the protein-ligand binding

Cited by 5 publications

References 4 publications

Asymmetric immobilization of antibodies on a piezo-resistive micro-cantilever surface

Asymmetric immobilization of antibodies on a piezo-resistive micro-cantilever surface

A Genosensor Based on the Modification of a Microcantilever: A Review

Fundamentals and current status of polymeric piezoresistive cantilever technology applied on biosensors

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