Fabrication and characterization of collagen-based injectable and self-crosslinkable hydrogels for cell encapsulation

Gao, Yunkai; Kong, Weili; Li, Bao; Ni, Yilu; Yuan, Tun; Guo, Likun; Lin, Hai; Fan, Hongsong; Fan, Yujiang; Zhang, Xingdong

doi:10.1016/j.colsurfb.2018.04.009

Cited by 62 publications

(27 citation statements)

References 38 publications

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“…A moist environment for wounds is conductive to keratinocyte migration and epithelialization. 47 In addition, thermo-sensitive hydrogel has strong shape adaptation after gelation and is similar to extracellular matrix, 48 which is beneficial to hydrogel-tissue adhesiveness, cell adhesion, proliferation, migration and differentiation. Moreover, the hydrogels could be directly applied at the wound site and had no side effects on organisms.…”

Section: Discussionmentioning

confidence: 99%

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Dong¹,

Zhuang²,

Hao³

et al. 2020

IJN

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Introduction: Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair. Methods: The purpose of this research was to prepare a novel thermo-sensitive hydrogelpoly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining. Results: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model. Discussion: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.

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Section: Discussionmentioning

confidence: 99%

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Dong¹,

Zhuang²,

Hao³

et al. 2020

IJN

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“…Collagen is widely present in extracellular matrix (ECM), owning plentiful advantages over other scaffold materials in terms of low antigenicity, good biocompatibility, more cell recognition sites, which facilitates cell adhesion and proliferation. Gao et al [28] fabricated excellent cell carrier based on collagen type I and activated chondroitin sulfate by physical and chemical crosslinking without the addition of any catalysts. Furthermore, Zhang et al [29] designed a biocompatible bone repair material prepared from crosslinked porous constructs of collagen and hydroxyapatite, the in vitro and in vivo experiments results demonstrated the novel materials presented high biocompatibility and effectively induced osteanagenesis ability.…”

Section: Introductionmentioning

confidence: 99%

Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds

Cao

Lin

Cai

et al. 2019

Regenerative Biomaterials

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Mechanical signals have been played close attention to regulate chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In this study, dynamic mechanical loading simulation with natural frequencies and intensities were applied to the 3D cultured BMSCs–collagen scaffold constructs. We investigated the effects of dynamic mechanical loading on cell adhesion, uniform distribution, proliferation, secretion of extracellular matrix (ECM) and chondrogenic differentiation of BMSCs–collagen scaffold constructs. The results indicated that dynamic mechanical loading facilitated the BMSCs adhesion, uniform distribution, proliferation and secretion of ECM with a slight contraction, which significantly improved the mechanical strength of the BMSCs–collagen scaffold constructs for better mimicking the structure and function of a native cartilage. Gene expression results indicated that dynamic mechanical loading contributed to the chondrogenic differentiation of BMSCs with higher levels of AGG, COL2A1 and SOX9 genes, and prevented of hypertrophic process with lower levels of COL10A1, and reduced the possibility of fibrocartilage formation due to down-regulated COL1A2. In conclusion, this study emphasized the important role of dynamic mechanical loading on promoting BMSCs chondrogenic differentiation and maintaining the cartilage phenotype for in vitro reconstruction of tissue-engineered cartilage, which provided an attractive prospect and a feasibility strategy for cartilage repair.

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“…Swelling characteristics of human collagen hydrogels were determined by employing a previously published protocol. 20 Collagen hydrogels were first dried under vacuum and the dry weight of the hydrogel was measured ( W d ). Following this, the hydrogels were immersed in 500 µL of 1× PBS at 37°C for 24 h. Excess liquid was removed by gently blotting the hydrogel on a Kimwipe, and the wet weight ( W w ) was measured.…”

Section: Methodsmentioning

confidence: 99%

In vitro characterization of xeno-free clinically relevant human collagen and its applicability in cell-laden 3D bioprinting

et al. 2020

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Collagen type I, commonly derived from xenogenic sources, is extensively used as a biomaterial for tissue engineering applications. However, the use of xenogenic collagen is typically associated with species specific variation in mechanical, structural, and biological properties that are known to influence cellular response and remodeling. In addition, immunological complications and risks of disease transmission are also major concerns. The goal of this study is to characterize a new xeno-free human skin-derived collagen and assess its applicability as a bioink for cell-laden 3 D bioprinting. Four different concentrations of human collagen (i.e., 0.5 mg/mL, 1 mg/mL, 3 mg/mL and 6 mg/mL) were employed for the synthesis of collagen hydrogels. In addition, bovine collagen was used as a xenogenic control. Results from SDS-PAGE analysis showed the presence of α1, α2, and β chains, confirming that the integrity of type I human collagen is maintained post isolation. Polymerization rate and compressive modulus increased significantly with increase in the concentration of human collagen. When comparing two different sources of collagen, the polymerization rate of xenogenic collagen was significantly faster (p < 0.05) than human collagen while the compressive modulus was comparable. Raman spectroscopy showed a large peak in the Amide I band around 1600 cm−1, indicating a dense and supraorganized fibrillar structure in human collagen hydrogels. Conversely, Amide I band intensity for xenogenic collagen was comparable to that of Amide II and Amide III bands. Further, the use of 6 mg/mL human collagen as a bioink yielded 3 D printed constructs with high shape fidelity and cell viability. On the other hand, xenogenic collagen failed to yield stable 3 D printed constructs. Together, the results from this study provides an impetus for using human-derived collagen as a viable alternative to xenogenic sources for 3 D bioprinting of clinically relevant scaffolds for tissue engineering applications.

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Fabrication and characterization of collagen-based injectable and self-crosslinkable hydrogels for cell encapsulation

Cited by 62 publications

References 38 publications

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds

In vitro characterization of xeno-free clinically relevant human collagen and its applicability in cell-laden 3D bioprinting

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