Fabrication and characterization of epithelial scaffolds for hair follicle regeneration

Oh, Ji Won; Choi, Ju Young; Kim, Minji; Abdi, Syed Izhar Haider; Lau, Hui Chong; Kim, Moonkyu; Lim, Jeong Ok

doi:10.1007/s13770-012-0147-9

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Recently, our group has developed a method to recover trichogenecity of epithelial cells in 3D cultivation using a biodegradable scaffold [24,40]. We used sponge-type, fiber sheet and newborn epithelial skin scaffold (NESS) to restore the hair follicle formation properties of the cells.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Human Dermal Papilla Cells in Alginate Spheres

et al. 2018

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Maintenance of trichogenecity of dermal papilla cells (DPCs) have been a problem during cell therapy for androgenic alopecia, as they lose their regenerative potential in in vitro culture. Various spheroid culture techniques are used to increase and maintain trichogenecity of these cells. However, there are some critical drawbacks in these methods. Applying a hydrocell plate for sphere formation or hanging drop methods by hand would be difficult to control the size and cell density inside it. It would be difficult to commercialize or mass production for clinical therapy. In aim to address and overcome these drawbacks, we have introduced alginate sphere. The alginate sphere of DPCs were prepared by electrospinning at different voltages to control the size of sphere. Then the obtained alginate spheres were evaluated for cellular dynamics and density of DPCs under different conditions. In this study, we found that DPCs do not proliferate in alginate sphere. However, the number of DPCs were maintained and found to be in dormant state. Further, the dormant DPCs in the alginate sphere have upregulated DPC signature genes (SOX2, ALPL, WIF1, Noggin, BMP4 and VCAN) and proliferative capacity. Thus, we speculate that alginate sphere environment maintains the dormancy of DPCs with increased trichogenecity.

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Section: Discussionmentioning

confidence: 99%

“…Informed written consent was obtained from the patients. The hair follicles were isolated and cultured by the method described with minor modifications [24]. Briefly, the subcutaneous fat of the scalp skin, including the lower hair follicles, was dissected from the epidermis and dermis.…”

Section: Isolation Of Human Hair Folliclesmentioning

confidence: 99%

Characterization of Human Dermal Papilla Cells in Alginate Spheres

et al. 2018

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“…Along these lines, we can find Wnt signaling pathway, a critical developmental signaling mechanism of fat and hair, is also highlighted in up-regulated DEGs in macrodactyly. Further study including three dimension tissue scaffold experiment [28] or organotypic skin culture with adipose tissue [29] can elucidate the novel mechanistic correlation between soft tissue enlargement of macrodactyly finger and sparse density of hair follicles at the finger.…”

Section: Discussionmentioning

confidence: 99%

Signature Genes in Macrodactyly through Transcriptome Network Analysis Reveal their Association of Lipid Metabolism

Choi

Lee

2017

Korean J Phys Anthropol

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: Macrodactyly is one of the most difficult hand anomalies to treat not only surgically but medically as well. Little is known about the molecular pathways and lipid metabolism of this disease. To elucidate the potential mechanism of macrodactyly progress, we used the bioinformatical analysis including quantile normalization, principal component analysis, heatmap and volcano plot. For the functional bioinformatical study, lipid, lipoprotein and phospholipid metabolism of Kyoto Encyclopedia of Genes and Genomes, Wiki Pathways, and Reactome Pathway were utilized to compare the differentially expressed genes in macrodactyly with control group. We found up-regulation of CDK6 and E2F1, which are associated with the mitotic cell cycle of cancer cells. PIK3CG, associated with cancer and lipid metabolism, was also enriched in macrodactyly. In down-regulated genes, PTEN was highlighted in lipid metabolism, phosphatidylinositol signaling system and insulin signaling. ABCD3, related in peroxisomal import of fatty acids, was also down-regulated. In this study, we predicted the pathogenic candidate genes as well as the potential molecular pathways related to macrodactyly by identifying the signature genes. Signature genes through systems bioinformatical analysis can be utilized to catch the insight of the molecular pathogenesis of macrodactyly.

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