Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering

Shahbazarab, Zeinab; Teimouri, Abbas; Chermahini, Alireza Najafi; Azadi, Mohammad

doi:10.1016/j.ijbiomac.2017.11.017

Cited by 87 publications

(47 citation statements)

References 53 publications

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“…These include (i) bioceramics such as hydroxyl apatite 22 , (ii) natural macromolecules and their derivatives such as collagen, atelocollagen, chitosan and alginate 23 – 25 , (iii) synthetic polymers such as polyethylene glycol 26 , (iv) hydrogels 27 , 28 , and (v) other materials such as carbon nanotubes 29 , 30 . A variety of combinations of (i~v) have also been widely used 20 , 21 , 31 – 33 . They can be shaped into gel-like, sponge-like, mesh-like, or fibrous structures.…”

Section: Discussionmentioning

confidence: 99%

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Satô

Yamamoto

Horiguchi

et al. 2018

Sci Rep

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Transplantation of engineered three-dimensional (3D) bone tissue may provide therapeutic benefits to patients with various bone diseases. To achieve this goal, appropriate 3D scaffolds and cells are required. In the present study, we devised a novel nanogel tectonic material for artificial 3D scaffold, namely the nanogel-cross-linked porous (NanoCliP)-freeze-dried (FD) gel, and estimated its potential as a 3D scaffold for bone tissue engineering. As the osteoblasts, directly converted osteoblasts (dOBs) were used, because a large number of highly functional osteoblasts could be induced from fibroblasts that can be collected from patients with a minimally invasive procedure. The NanoCliP-FD gel was highly porous, and fibronectin coating of the gel allowed efficient adhesion of the dOBs, so that the cells occupied the almost entire surface of the walls of the pores after culturing for 7 days. The dOBs massively produced calcified bone matrix, and the culture could be continued for at least 28 days. The NanoCliP-FD gel with dOBs remarkably promoted bone regeneration in vivo after having been grafted to bone defect lesions that were artificially created in mice. The present findings suggest that the combination of the NanoCliP-FD gel and dOBs may provide a feasible therapeutic modality for bone diseases.

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Section: Discussionmentioning

confidence: 99%

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Satô

Yamamoto

Horiguchi

et al. 2018

Sci Rep

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“…With the addition of gelatin to chitosan, the microporous structure is maintained, as presented in Figure 7, but some small fibers are observed to grow on the chitosan surface and in some of the pores, conferring compactness. The porous structures of chitosan and gelatin play an important role in bone formation, because they allow vascularization, as well as migration and proliferation of osteoblasts and mesenchymal cells, and therefore, they can be successfully used as a support in the final scaffold for tissue engineering [50,51]. The SEM micrographs and EDS spectrum of the final HAp(ESM)_CS_Gel_BA biocomposite scaffold are presented in Figure 8.…”

Section: Composite Scaffold and Saffold Component Characterizationmentioning

confidence: 99%

Biomimetic Composite Scaffold Based on Naturally Derived Biomaterials

et al. 2020

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This paper proposes the development of a biomimetic composite based on naturally derived biomaterials. This freeze-dried scaffold contains a microwave-synthesized form of biomimetic hydroxyapatite (HAp), using the interwoven hierarchical structure of eggshell membrane (ESM) as bio-template. The bone regeneration capacity of the scaffold is enhanced with the help of added tricalcium phosphate from bovine Bone ash (BA). With the addition of Gelatin (Gel) and Chitosan (CS) as organic matrix, the obtained composite is characterized by the ability to stimulate the cellular response and might accelerate the bone healing process. Structural characterization of the synthesized HAp (ESM) confirms the presence of both hydroxyapatite and monetite phases, in accordance with the spectroscopy results on the ESM before and after the microwave thermal treatment (the presence of phosphate group). Morphology studies on all individual components and final scaffold, highlight their morphology and porous structure, characteristics that influence the biocompatibility of the scaffold. Porosity, swelling rate and the in vitro cytotoxicity assays performed on amniotic fluid stem cells (AFSC), demonstrate the effective biocompatibility of the obtained materials. The experimental results presented in this paper highlight an original biocomposite scaffold obtained from naturally derived materials, in a nontoxic manner.

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“…Several types of 3D scaffolds for bone tissue engineering have been reported in the literature [ 18 , 19 ], including hydroxyapatite bioceramics [ 20 ], collagen, atelocollagen, chitosan and alginate (also known as natural macromolecules and their derivatives) [ 21 , 22 , 23 ], polyethylene glycol (synthetic polymer) [ 24 ], hydrogels [ 25 , 26 ], and other materials, such as carbon nanotubes [ 26 , 27 ]. These materials have also been used in combination to achieve superior properties [ 19 , 28 , 29 , 30 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…This may be due to a high concentration of nanogel in the porous locations. Previous studies showed that soluble molecules, including BMP [ 32 , 34 ], FGF18 [ 34 ], EP4A [ 33 ], and W9 peptide [ 35 ], promote bone regeneration in vivo. These studies reported the unusual stimulation of periosteal progenitor cells, termed the periosteal reaction, contributing to cartilage callus [ 36 ] or fibrocartilage formation [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

et al. 2020

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The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.

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Fabrication and characterization of nanobiocomposite scaffold of zein/chitosan/nanohydroxyapatite prepared by freeze-drying method for bone tissue engineering

Cited by 87 publications

References 53 publications

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Biomimetic Composite Scaffold Based on Naturally Derived Biomaterials

In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

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