Fabrication and Investigation of the Suitability of Chitosan-Silver Composite Scaffolds for Bone Tissue Engineering Applications

Vaidhyanathan, Baskaran; Vincent, Preethi; Vadivel, Sajini; Karuppiah, Ponmurugan; Al-Dhabi, Naïf Abdullah; Sadhasivam, Deepa Rani; Vimalraj, Selvaraj; Sekaran, Saravanan

doi:10.1016/j.procbio.2020.10.008

Cited by 49 publications

(17 citation statements)

References 41 publications

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“…The previous report concluded that a strong interaction force between PCL and AgNPs interface contributes to heightened stiffness/Young’s modulus [ 63 ]. Increment of mechanical properties of polymeric scaffold after addition of filler was observed not only in PCL/AgNPs composite, but also in other materials with similar concepts such as PCL/multi-walled carbon nanotubes (MWCNT) [ 17 ], PCL/PANI [ 6 ], and Chitosan/Ag [ 64 ] bone scaffolds. In addition, the incorporation of AgNPs was also shown to reduce the scaffold’s porosity from 70 ± 34% (PCL scaffold; Table 3 ) to 60 ± 4% (PCL/AgNPs scaffold; Table 3 ), which is known to be inversely proportional to the scaffold’s compressive strength (i.e., scaffold compressive strength will increase when scaffold porosity decreases) [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

et al. 2021

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Electroactive biomaterials are fascinating for tissue engineering applications because of their ability to deliver electrical stimulation directly to cells, tissue, and organs. One particularly attractive conductive filler for electroactive biomaterials is silver nanoparticles (AgNPs) because of their high conductivity, antibacterial activity, and ability to promote bone healing. However, production of AgNPs involves a toxic reducing agent which would inhibit biological scaffold performance. This work explores facile and green synthesis of AgNPs using extract of Cilembu sweet potato and studies the effect of baking and precursor concentrations (1, 10 and 100 mM) on AgNPs’ properties. Transmission electron microscope (TEM) results revealed that the smallest particle size of AgNPs (9.95 ± 3.69 nm) with nodular morphology was obtained by utilization of baked extract and ten mM AgNO3. Polycaprolactone (PCL)/AgNPs scaffolds exhibited several enhancements compared to PCL scaffolds. Compressive strength was six times greater (3.88 ± 0.42 MPa), more hydrophilic (contact angle of 76.8 ± 1.7°), conductive (2.3 ± 0.5 × 10−3 S/cm) and exhibited anti-bacterial properties against Staphylococcus aureus ATCC3658 (99.5% reduction of surviving bacteria). Despite the promising results, further investigation on biological assessment is required to obtain comprehensive study of this scaffold. This green synthesis approach together with the use of 3D printing opens a new route to manufacture AgNPs-based electroactive with improved anti-bacterial properties without utilization of any toxic organic solvents.

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Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

et al. 2021

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“…The development of innovative techniques and new biomaterials to fabricate porous, osteogenic, osteoconductive, osteoinductive, non-toxic, and biodegradable implants [ 1 , 2 ] with adequate mechanical strength is a challenge for many scientists, doctors, and engineers in the repair and treatment of bone tissue damaged by cancer, osteomyelitis, congenital defects, or accidents [ 3 , 4 ]. Composites based on ceramics (e.g., bioglasses, silica, hydroxyapatite, and titian), polymers (both natural and synthetic such as chitosan, collagen, fibrin, elastin, alginate, hyaluronic acid, polylactic acid) and hybrid bio-composites [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ] are mainly used in bone tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

“…Its low toxic effects and biological inertness have been confirmed in studies, both in vitro and in vivo [ 21 ]. However, scaffolds made only with chitosan as the base polymer are characterized by poor mechanical properties including poor tensile strength and low fracture stiffness, fast degradation rate, and low osteoconductivity [ 4 ]. Moreover, it is difficult to control pore size during the fabrication of chitosan implants, which play a key role in osteoblast survival, growth, and differentiation [ 22 ] as well as transport of essential components necessary for bone regeneration, gas diffusion, and removal of metabolism products.…”

Section: Introductionmentioning

confidence: 99%

Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering

Adamski

Siuta

2021

Molecules

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The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol–gel method. The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young’s modulus, compressive strength, and yield strain, of hybrid composites was analyzed. The microstructure of the materials obtained was visualized by SEM. Moreover, the molecular interactions according to FTIR analysis and biocompatibility of composites obtained were examined. The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young’s modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%. This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.

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“…Particular attention has to be paid to the use chitosan (CS) in tissue engineering [ 69 ] and in self-healing [ 70 ] magnetic triggered supports [ 71 ]. In fact, the cytocompatibility and biodegradability of chitosan even when mixed with gold [ 72 ], silver [ 73 ] and iron oxide nanoparticles proposed this natural polymer as a suitable candidate for tissue engineering. CS is largely used in a mixture with several materials [ 74 ], including hydroxyapatite [ 75 ].…”

Section: Magneto-responsive Scaffolds For Hard Tissue Regenerationmentioning

confidence: 99%

Paramagnetic Functionalization of Biocompatible Scaffolds for Biomedical Applications: A Perspective

et al. 2020

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The burst of research papers focused on the tissue engineering and regeneration recorded in the last years is justified by the increased skills in the synthesis of nanostructures able to confer peculiar biological and mechanical features to the matrix where they are dispersed. Inorganic, organic and hybrid nanostructures are proposed in the literature depending on the characteristic that has to be tuned and on the effect that has to be induced. In the field of the inorganic nanoparticles used for decorating the bio-scaffolds, the most recent contributions about the paramagnetic and superparamagnetic nanoparticles use was evaluated in the present contribution. The intrinsic properties of the paramagnetic nanoparticles, the possibility to be triggered by the simple application of an external magnetic field, their biocompatibility and the easiness of the synthetic procedures for obtaining them proposed these nanostructures as ideal candidates for positively enhancing the tissue regeneration. Herein, we divided the discussion into two macro-topics: the use of magnetic nanoparticles in scaffolds used for hard tissue engineering for soft tissue regeneration.

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Fabrication and Investigation of the Suitability of Chitosan-Silver Composite Scaffolds for Bone Tissue Engineering Applications

Cited by 49 publications

References 41 publications

Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering

Paramagnetic Functionalization of Biocompatible Scaffolds for Biomedical Applications: A Perspective

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