Fabrication and packaging of a mass-producible capillary-assembled microchip for simple and multiplexed bioassay

Henares, Terence G.; Shirai, Atsushi; Sueyoshi, Kenji; Endo, Tatsuro; Hisamoto, Hideaki

doi:10.1016/j.snb.2015.05.001

Cited by 10 publications

(4 citation statements)

References 36 publications

(44 reference statements)

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“…Capillary-tube assay systems, in spite of their simple structure, have not been as successful for POC purposes. Although some capillary systems have been reported with emphasis on achieving a low limit of detection (LOD) [ 4 , 5 , 6 ], the single-step process [ 6 , 7 , 8 ] and multi-analyte detection [ 9 , 10 ], those capillary systems were mostly not as successful due either to LOD [ 4 , 5 , 7 , 8 , 10 ] or to complicated fabrication steps [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Kim

Cho

Jeong

et al. 2017

Sensors

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Using gold nanoparticles (AuNPs) on “capillary enzyme-linked immunosorbent assay (ELISA)”, we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of AuNPs, AuNPs of varying diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm, and 50 nm) conjugated with Horseradish Peroxidase (HRP)-labeled anti-C reactive protein (antiCRP) (AuNP•antiCRP-HRP) were used for well-plate ELISA. AuNP of 10 nm produced the largest optical density, enabling detection of 0.1 ng/mL of CRP with only 30 s of incubation, in contrast to 10 ng/mL for the ELISA run in the absence of AuNP. Then, AuNP of 10 nm conjugated with antiCRP-HRP (AuNP•antiCRP-HRP) was used for “capillary ELISA” to detect as low as 0.1 ng/mL of CRP. Also, kinetic study on both 96-well plates and in a capillary tube using antiCRP-HRP or AuNP•antiCRP-HRP showed a synergistic effect between AuNP and the capillary system, in which the fastest assay was observed from the “AuNP capillary ELISA”, with its maximum absorbance reaching 2.5 min, while the slowest was the typical well-plate ELISA with its maximum absorbance reaching in 13.5 min.

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Section: Introductionmentioning

confidence: 99%

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Kim

Cho

Jeong

et al. 2017

Sensors

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“…Furthermore, the present microdevice can be used not only for single-step and fast measurement of various proteins, but also for simultaneous measurement of various analytes such as metabolites, enzyme activities, and ions by simply arraying capillary-type microdevices developed previously in our group. 53…”

Section: Discussionmentioning

confidence: 99%

Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide–antibody conjugate and fluorescently labelled antibody

Shirai

Henares

Sueyoshi

et al. 2016

Analyst

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In this study, a fast and easy-to-use capillary-type microdevice for competitive bioassay is proposed. The device is composed of polydimethylsiloxane (PDMS) microchannel arrays that separately immobilize polyethylene glycol (PEG) coating, which contained graphene oxide (GO)-analyte conjugates and fluorescently-labelled receptor proteins. The working principle of the device involved the spontaneous dissolution of the PEG coating, subsequent mixing and reaction with analyte to give fluorescence response, triggered by the capillary action-mediated introduction of a sample solution. For principle verification, a competitive biotin assay was successfully demonstrated within 20 s in a single-step operation by detecting the change in fluorescence via microscopy.

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“…[5][6][7][8] However, simultaneously solving these problems is still challenging. Recently, we proposed designing "singlestep" microdevices based on square glass capillaries [9][10][11][12][13] or polymer capillaries. [14][15][16] For these approaches, the inner capillary wall is functionalized by physical adsorption or covalent bonding of analytical reagents or by coating the wall with reagent-containing films.…”

Section: Introductionmentioning

confidence: 99%

Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide-Analyte Conjugates and Fluorescently-labelled Receptor Proteins

et al. 2017

Self Cite

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Fabrication and packaging of a mass-producible capillary-assembled microchip for simple and multiplexed bioassay

Cited by 10 publications

References 36 publications

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide–antibody conjugate and fluorescently labelled antibody

Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide-Analyte Conjugates and Fluorescently-labelled Receptor Proteins

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