Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers

Abstract

A gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption–desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin sulfate (GNT) in phosphate-buffered saline (PBS) at room temperature. After 6 h mixing, the GNT adsorption in all conditions reached plateau by Langmuir’s isotherm, and maximum GNT adsorption amount was 34 ± 7 μg in 250 μg/mL GNT solution. Saturated GNT-loaded HAp/Col powder of 100 mg was soaked in 10 mL of PBS at 37 °C and released all GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to Escherichia coli (E. coli) at least for 2 days. From the release profile of the GNT from the GNT-HAp/Col powder, antibacterial efficacy would affect E. coli at least for 3 days. Further, no cytotoxicities were observed on MG-63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresorbable anti-infection bone void fillers by prevention initial infections, which is the primary cause of implant-associated infection even for rapid bioresorbable materials.

Local treatment of experimental mandibular osteomyelitis with an injectable biomimetic gentamicin hydrogel using a new rabbit model

“…Both COLL hydrogels were functionalized with GNT for the prevention and treatment of infection in osteomyelitis based on literature reports 42‐44 . Almost complete release of GNT from the GNT‐COLL/nHA hydrogel under physiological conditions (PBS pH 7.4, 37°C, Figure 3c) was achieved in 4 days, in line with literature data 45 . The in vitro study findings suggested potential of the hydrogels for the local treatment of mandibular OM, which has not been documented to date.…”

New Approach to Antifungal Activity of Fluconazole Incorporated into the Porous 6-Anhydro-α-l-Galacto-β-d-Galactan Structures Modified with Nanohydroxyapatite for Chronic-Wound Treatments—In Vitro Evaluation

“…The most frequently studied are hydroxyapatites substituted with ions and many of them have documented antimicrobial activity [ 8 , 9 ]. Another concept is combining hydroxyapatite and antibiotics, in which antimicrobial drug is released in the area of the application site where it inhibits microbial adhesion and growth [ 10 ].…”

Review of the Applications of Biomedical Compositions Containing Hydroxyapatite and Collagen Modified by Bioactive Components

“…It is worth noticing that a growing interest in multicomponent systems containing gentamycin and other antibiotics, hydroxyapatite, and collagen is currently observed. Such systems aim at the formation of the material providing the stable release of antibiotics while supporting bone-tissue growth [ 148 , 149 ]. For example, Ionescu et al [ 150 ] performed the synthesis of biocomposite materials consisting of hydroxyapatite, gentamycin, and collagen.…”