Fabrication of microfluidic mixers with varying topography in glass using the powder-blasting process

Sayah, A.; Thivolle, Pierre-Antoine; Parashar, V.K.; Gijs, Martin A. M.

doi:10.1088/0960-1317/19/8/085024

Cited by 18 publications

(16 citation statements)

References 30 publications

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“…White cotton-based yarn has been used to fabricate elements of a complex microfluidic circuit (24). 3D channels in glass, fabricated using a powder blasting technology, with varying channel topography has been used as a microfluidic mixer by Gijs and co-workers (25). Klein and co-workers fabricated solenoidal microcoils with a hollow core in borosilicate glass using an anodic wire bonder (26).…”

Section: Technologymentioning

confidence: 99%

Latest Developments in Micro Total Analysis Systems

et al. 2010

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The field of micro total analysis systems (µTAS) (1) or lab on a chip (LOC) has extended its usefulness into many new fields and disciplines spanning basic research to commercial applications. The importance of µTAS is reflected in both the growing number and improved quality of published articles. µTAS has expanded into an incredibly diverse number of analytical chemistry applications and has received a great amount of input from a wide spectrum of scientific and engineering disciplines. In this sense, µTAS is highly interdisciplinary and has served as a focal point to bring together multidisciplinary research fields.This work builds on former reviews (2-6) and attempts to chart the most recent developments in µTAS. By cross referencing online keyword searches, several thousand articles were found spread among a wide variety of journals but were most frequently found in the high impact journals such as Science, Nature, PNAS, Analytical Chemistry, Lab on a Chip, and others. The annual international µTAS conference also provided articles on some of the very latest and most promising developments. This review article aims to report the latest achievements in the field of µTAS by highlighting some of the very best research papers published over the 2 year period between March 2008 and February 2010. Our main goal is to give a broad overview of the latest state-ofthe-art research and to introduce newcomers to the field. We were overwhelmed by the large number of research papers published on cellular analysis, manipulation, and droplet fluidics; therefore, we divided the review into two parts. This first part concentrates on reports related to manufacturing, technology, and instrumentation for molecular analysis, and part two covers applications related to cellular analysis and manipulation, which is referred to as cellson-a-chip (460). The papers we selected on droplet fluidics are distributed throughout both parts of the article according to their applications. We attempted to select papers based on the merit of their contents, which was a difficult task, and we have missed some good articles; for these oversights, we offer our apology in advance.Since this review focuses on novel developments of microfluidic systems for applied analytical chemical applications, publications about sensor arrays (so-called "biochips"), chemical synthesis on microchips (except some papers on particle synthesis using droplets), theory, and simulations, as well as trend articles, have been omitted. Furthermore, we reduced the technology section, as the majority of work is carried out by employing simple planar microchips fabricated in glass or polymers such as silicone elastomers. TECHNOLOGYMicrofabrication. Conventional Microfabrication. Many new innovative approaches have been reported in the field of microfabrication. Semicircular cross section channels network microfabricated on a silicon chip by XeF 2 etching by Camp et al. (7). These channels allow stress free transportation of cells. Generally, vapor deposited thin film metal ele...

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Section: Technologymentioning

confidence: 99%

Latest Developments in Micro Total Analysis Systems

et al. 2010

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“…To isolate the microalgal strains having high biomass and lipid productivities, efficient screening systems have been developed based on microfluidics (J. Y. H. Kim et al, ; Sung, Kwak, Hong, Choi, & Sim, ), microdroplets (Sung, Kim, Choi, Kwak, & Sim, ), and fluorescence‐based flow cytometry (Terashima, Freeman, Jinkerson, & Jonikas, ). However, while these platforms enable high‐throughput screening of cells, they are accompanied by many disadvantages, including dependence on fluid and surface properties (van Dam, ), requirement of multiple fabrication steps (Sayah, Thivolle, Parashar, & Gijs, ), and requirement of sophisticated equipment (Visser, Kamperman, Karbaat, Lohse, & Karperien, ).…”

Section: Introductionmentioning

confidence: 99%

Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation

Sung

Choi

Lee

et al. 2019

Biotech & Bioengineering

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Microalgae have a high potential to be utilized as feedstock for biofuels because they have high growth rates and do not compromise food production. Commercialized algae-based biofuel production relies on the development of strains with high lipid content. Based on the relatively low density of lipids compared to other cellular components, density gradient centrifugation was used to isolate high lipid content algal strains from Chlamydomonas reinhardtii mutant libraries. The correlation between cell density and lipid content was confirmed by analysis of Nile red fluorescence intensity, total lipids, and total fatty acid methyl ester content. A strain isolated by this screening method had 50% higher lipid content and 7% lower cell density than the parent wild-type strain. Consequently, we demonstrated that screening of algal strains with low cell density via continuous density gradient centrifugation allows simple, rapid, and inexpensive screening for high lipid content strains. K E Y W O R D S cell screening system, density gradient centrifugation, high lipid content strain, microalgae

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“…Alternative methods of producing multi-level channels such as hot embossing 4 , erosion 5 , etching 6 , laser fabrication 7 , photoresist reflow 8 , micro-milling 9 , 3D-printing 10 , electroplating 11 , deformable molds 12 , and liquid molding 13 are also laborious and/or require specialized equipment.…”

Section: Introductionmentioning

confidence: 99%

Simple multi-level microchannel fabrication by pseudo-grayscale backside diffused light lithography

et al. 2013

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Photolithography of multi-level channel features in microfluidics is laborious and/or costly. Grayscale photolithography is mostly used with positive photoresists and conventional front side exposure, but the grayscale masks needed are generally costly and positive photoresists are not commonly used in microfluidic rapid prototyping. Here we introduce a simple and inexpensive alternative that uses pseudo-grayscale (pGS) photomasks in combination with backside diffused light lithography (BDLL) and the commonly used negative photoresist, SU-8. BDLL can produce smooth multi-level channels of gradually changing heights without use of true grayscale masks because of the use of diffused light. Since the exposure is done through a glass slide, the photoresist is cross-linked from the substrate side up enabling well-defined and stable structures to be fabricated from even unspun photoresist layers. In addition to providing unique structures and capabilities, the method is compatible with the “garage microfluidics” concept of creating useful tools at low cost since pGS BDLL can be performed with the use of only hot plates and a UV transilluminator: equipment commonly found in biology labs. Expensive spin coaters or collimated UV aligners are not needed. To demonstrate the applicability of pGS BDLL, a variety of weir-type cell traps were constructed with a single UV exposure to separate cancer cells (MDA-MB-231, 10-15 μm in size) from red blood cells (RBCs, 2-8 μm in size) as well as follicle clusters (40-50 μm in size) from cancer cells (MDA-MB-231, 10-15 μm in size).

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Fabrication of microfluidic mixers with varying topography in glass using the powder-blasting process

Cited by 18 publications

References 30 publications

Latest Developments in Micro Total Analysis Systems

Latest Developments in Micro Total Analysis Systems

Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation

Simple multi-level microchannel fabrication by pseudo-grayscale backside diffused light lithography

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