Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process

Autissier, Aude; Visage, Catherine Le; Pouzet, Cécile; Chaubet, Frédéric; Letourneur, Didier

doi:10.1016/j.actbio.2010.03.004

Cited by 165 publications

(99 citation statements)

References 39 publications

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“…Here, we used a 3D porous scaffold made of pullulan and dextran polysaccharides (Le Visage, 2009a;Le Visage, 2009b) for bone tissue engineering applications. This biomaterial has already been successfully used in vitro for cardiovascular engineering applications (Autissier et al, 2007;Thebaud et al, 2007;Autissier et al, 2010). Furthermore, its porosity is compatible with the ingrowth of bone tissue (Karageorgiou and Kaplan, 2005) and our preliminary data showed that human ADSCs were able to differentiate into osteogenic cells within these 3D matrices and to express in vitro bone specifi c markers.…”

Section: Introductionmentioning

confidence: 51%

Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering

Lalande

Miraux²,

Derkaoui³

et al. 2011

eCM

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For bone tissue engineering, human Adipose Derived Stem Cells (hADSCs) are proposed to be associated with a scaffold for promoting bone regeneration. After implantation, cellularised scaffolds require a non-invasive method for monitoring their fate in vivo. The purpose of this study was to use Magnetic Resonance Imaging (MRI)-based tracking of these cells, labelled with magnetic agents for in vivo longitudinal assessment. hADSCs were isolated from adipose tissue and labelled with USPIO-rhodamine (Ultrasmall SuperParamagnetic Iron Oxide). USPIO internalisation, absence of toxicity towards hADSCs, and osteogenic differentiation of the labelled cells were evaluated in standard culture conditions. Labelled cells were then seeded within a 3D porous polysaccharidebased scaffold and imaged in vitro using fl uorescence microscopy and MRI. Cellularised scaffolds were implanted subcutaneously in nude mice and MRI analyses were performed from 1 to 28 d after implantation. In vitro, no effect of USPIO labelling on cell viability and osteogenic differentiation was found. USPIO were efficiently internalised by hADSCs and generated a high T2* contrast. In vivo MRI revealed that hADSCs remain detectable until 28 d after implantation and could migrate from the scaffold and colonise the area around it. These data suggested that this scaffold might behave as a cell carrier capable of both holding a cell fraction and delivering cells to the site of implantation. In addition, the present fi ndings evidenced that MRI is a reliable technique to validate cell-seeding procedures in 3D porous scaffolds, and to assess the fate of hADSCs transplanted in vivo.

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Section: Introductionmentioning

confidence: 51%

Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering

Lalande

Miraux²,

Derkaoui³

et al. 2011

eCM

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“…However, in some papers, the PSD and other textural characteristics were determined in detail using mercury porosimetry [78] and analysis of images [3,76,[105][106][107][108]. There are several papers describing the PSD of native polymer cryogels over the total range of pore sizes from narrow nanopores (d nn < 2 nm) to macropores at d macro > 100…”

Section: Structural and Adsorption Characterisation Of Cryogelsmentioning

confidence: 99%

“…A more advanced analysis of microscopic images was used by Autissier et al [76]. They applied a combined freeze-drying/cross-linking process to prepare porous polysaccharide-based scaffolds using pullulan and dextran with sodium trimetaphosphate and showed that the degree of porosity of cryogels depends on freeze-drying pressure.…”

mentioning

confidence: 99%

“…2a). The porosity is an important parameter of performance of tissue scaffolds because the cells can penetrate deeper into scaffolds with larger pores [76]. However, the next step in deeper characterisation of the cryogel structures to obtain the PSD and other structural characteristics using image analysis of clear pictures ( Fig.…”

mentioning

confidence: 99%

“…However, the next step in deeper characterisation of the cryogel structures to obtain the PSD and other structural characteristics using image analysis of clear pictures ( Fig. 2a) was not done [76]. We calculated the PSD ( simplified approach in comparison with the techniques described in papers [105][106][107][108], vide infra).…”

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confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

287

205

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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Natural‐Based and Stimuli‐Responsive Polymers for Tissue Engineering and Regenerative Medicine

Oliveira

2014

Polymers in Regenerative Medicine

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Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process

Cited by 165 publications

References 39 publications

Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering

Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering

Cryogels: Morphological, structural and adsorption characterisation

Natural‐Based and Stimuli‐Responsive Polymers for Tissue Engineering and Regenerative Medicine

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